Probiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum

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dc.authoridAY, Mustafa/0000-0002-1765-4858
dc.authoridbagci, ufuk/0000-0002-1511-2465
dc.authorwosidÖZMEN TOĞAY, SINE/AAC-6337-2021
dc.authorwosidAY, Mustafa/IZQ-1342-2023
dc.authorwosidbagci, ufuk/JFA-0267-2023
dc.contributor.authorBagci, Ufuk
dc.contributor.authorTogay, Sine Ozmen
dc.contributor.authorTemiz, Ayhan
dc.contributor.authorAy, Mustafa
dc.date.accessioned2024-06-12T11:19:14Z
dc.date.available2024-06-12T11:19:14Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAs potential probiotic traits of human milk-isolated bacteria have increasingly been recognized, this study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum. Among 118 human milk- and colostrum-isolated lactic cocci, only 29 were identified as Enterococcus. Of these, only four Enterococcus faecium isolates exhibited bacteriocigenic activity against several pathogenic Gram-positive bacteria, including Listeria monocytogenes. These isolates exhibited high acid (up to pH 3.0) and bile tolerance (0.5% oxgall) in simulated gastrointestinal conditions, demonstrating their ability to survive through the upper gastrointestinal tract. All of the E. faecium strains were shown to be sensitive to most of the antibiotics including vancomycin, tetracycline, rifampicin, and erythromycin, while they were resistant to kanamycin and chloramphenicol. None of the strains showed any virulence (gelE, agg2, clyA, clyB, clyM) and antibiotic resistance genes (vanA, vanB, ermB, tetM, and aac(6')-le-aph(2 '')-la). In addition, all the strains were able to assimilate cholesterol, ranging between 25.2-64.1% and they exhibited variable adherence (19-36%) to Caco-2 cells. Based on the overall results of this in vitro study, four of the E. faecium strains isolated from human milk and colostrum can be considered as promising probiotic candidates; however, further in vivo evaluations are required.en_US
dc.description.sponsorshipHacettepe University Scientific Research Coordination Unit [4753]en_US
dc.description.sponsorshipAuthors would like to thank the Hacettepe University Scientific Research Coordination Unit (Project number: 4753) for financial support to this research project.en_US
dc.identifier.doi10.1007/s12223-019-00687-2
dc.identifier.endpage750en_US
dc.identifier.issn0015-5632
dc.identifier.issn1874-9356
dc.identifier.issue6en_US
dc.identifier.pmid30739237en_US
dc.identifier.scopus2-s2.0-85061305086en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage735en_US
dc.identifier.urihttps://doi.org/10.1007/s12223-019-00687-2
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25126
dc.identifier.volume64en_US
dc.identifier.wosWOS:000510846800004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofFolia Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLactic-Acid Bacteriaen_US
dc.subjectAntibiotic-Resistant Enterococcien_US
dc.subjectIn-Vitroen_US
dc.subjectCholesterol Assimilationen_US
dc.subjectAntimicrobial Resistanceen_US
dc.subjectGel-Electrophoresisen_US
dc.subjectOncorhynchus-Mykissen_US
dc.subjectVirulence Genesen_US
dc.subjectRainbow-Trouten_US
dc.subjectBreast-Milken_US
dc.titleProbiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrumen_US
dc.typeArticleen_US

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