Biological variation of peripheral blood T-lymphocytes

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dc.authoridZararsız, Gökmen/0000-0001-5801-1835
dc.authoridZARARSIZ, GOKMEN/0000-0001-5801-1835
dc.authoridKorkmaz, Selçuk/0000-0003-4632-6850
dc.authoridYucel, Dogan/0000-0001-5487-2857
dc.authoridyucel, cigdem/0000-0003-2647-440X
dc.authorwosidZararsız, Gökmen/E-8818-2013
dc.authorwosidKilinckaya, Muhammed/GLQ-7423-2022
dc.authorwosidZARARSIZ, GOKMEN/ABH-7959-2020
dc.authorwosidSenes, Mehmet/AAG-6802-2019
dc.authorwosidYucel, Dogan/B-3550-2018
dc.authorwosidKorkmaz, Selçuk/AAU-4677-2020
dc.authorwosidYucel, Dogan/AAM-3022-2021
dc.contributor.authorFalay, Mesude
dc.contributor.authorSenes, Mehmet
dc.contributor.authorKorkmaz, Selcuk
dc.contributor.authorZararsiz, Gokmen
dc.contributor.authorTurhan, Turan
dc.contributor.authorOkay, Murat
dc.contributor.authorYucel, Cigdem
dc.date.accessioned2024-06-12T10:54:39Z
dc.date.available2024-06-12T10:54:39Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Flow cytometric analysis of the lymphocyte subsets has become one of the most commonly used techniques in the routine clinical laboratory. It is frequently used in monitoring lymphocyte recovery after hematopoietic stem cell transplantation (HSCT), as well as diagnosis and treatment of acquired immunodeficiency syndrome (AIDS). Reliable biological variation (BV) data is needed for safe clinical application of these tests. In this study, similar preanalytical and analytical protocols to the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) checklist were followed and a stringent statistical approach was applied to define BV of T-lymphocytes. Methods: During the 10 weeks study period, weekly blood samples were obtained from 30 healthy individuals (20 females, 10 males) and analyzed with Facs Canto (BD Biosciences, San Jose, CA, USA) analyzer using 4colour BD Multitest CD3/CD8/CD45/CD4 reagents. Data were assessed in terms of normality, tendencies, outliers and variance homogeneity prior to applying coefficient of variance (CV)- analysis of variance (ANOVA) test. Sex-stratified within-individual (CVI) and between-individual (CVG) BV estimates of CD3+, CD3 + CD4 +, CD3 + CD8 +, and CD3 + CD4 + CD8 + T lymphocytes were calculated. Results: No difference was found between males and females. Except for the CD3 + CD4 + CD8 + subset, stable BV was found for CD3+, CD3 + CD4 +, and CD3 + CD8 + subsets. Conclussion: Instead of using the conventional reference ranges of CD3+, CD3 + CD4 + and CD3 + CD8 + counts for monitoring HIV positive or post-HSCT patients, RCV should be used. Because individualityis characteristic of lymphocytes subsets RCVS should be used instead of RIs for patient monitoring.en_US
dc.identifier.doi10.1016/j.jim.2019.04.002
dc.identifier.endpage5en_US
dc.identifier.issn0022-1759
dc.identifier.issn1872-7905
dc.identifier.pmid31022389en_US
dc.identifier.scopus2-s2.0-85064687655en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jim.2019.04.002
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19127
dc.identifier.volume470en_US
dc.identifier.wosWOS:000483322000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Immunological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiological Variationen_US
dc.subjectT Lymphocytesen_US
dc.subjectFlow Cytometryen_US
dc.subjectSubsetsen_US
dc.subjectAdultsen_US
dc.titleBiological variation of peripheral blood T-lymphocytesen_US
dc.typeArticleen_US

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