Genotoxic effects of diazinon on human peripheral blood lymphocytes
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Inst Medical Research & Occupational Health
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to evaluate the genetic damage in human peripheral blood lymphocytes following 24 and 48-hour exposure to a commercial diazinon formulation Basudin 60EM (R) at concentrations between 0.01 and 40 mu g mL(-1). For this purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), and alkaline single cell gel electrophoresis (comet) assay. Diazinon significantly increased the frequency of micronucleated cells compared to control. Forty-eight-hour exposure increased this frequency even at lower concentrations (0.01-10 mu g mL(-1)). The FISH results revealed aneugenic effects at 10 mu g mL(-1). The comet assay also confirmed DNA damage at concentrations between 10 and 40 mu g mL(-1). Our findings have confirmed the genotoxic potential of diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage in our study raises concern about the current assessment of the health risk posed by this pesticide and calls for a high level of caution in agricultural and household use.
Açıklama
Anahtar Kelimeler
Comet Assay, FISH, Genotoxicity, Micronucleus, Organophosphate Pesticides, Dna-Damage, Cells, Aneuploidy, Exposure, Induction, Workers
Kaynak
Arhiv Za Higijenu Rada I Toksikologiju-Archives Of Industrial Hygiene And Toxicology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
66
Sayı
2
