Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model

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dc.authoridKARACA, Turan/0000-0002-2500-7781;
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.authorwosidKara, Selcuk/C-8315-2015
dc.authorwosidkaraboga, ihsan/AAZ-9840-2020
dc.contributor.authorArikan, Sedat
dc.contributor.authorErsan, Ismail
dc.contributor.authorKaraca, Turan
dc.contributor.authorKara, Selcuk
dc.contributor.authorGencer, Baran
dc.contributor.authorKaraboga, Ihsan
dc.contributor.authorTufan, Hasan Ali
dc.date.accessioned2024-06-12T11:13:20Z
dc.date.available2024-06-12T11:13:20Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina. Methods: Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups. Results: The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 +/- 5.6 mu m and 16 +/- 6.4 mu m, respectively (P<0.05). Similarly, the mean thickness of ONL in the quercetin and ischemic groups was 50 +/- 12.8 mu m and 40 +/- 8.7 mu m, respectively (P<0.05). The antiapoptotic effect of quercetin in terms of reducing the numbers of both TUNEL (+) cells and caspase-3 (+) cells was significant in INL. The mean number of TUNEL (+) cells in INL in the ischemic and quercetin groups was 476.8 +/- 45.6/mm(2) and 238.72 +/- 251/mm(2), respectively (P<0.005). The mean number of caspase-3 (+) cells in INL of ischemic and quercetin groups was 633.6 +/- 38.7/mm(2) and 342.4 +/- 36.1/mm(2), respectively (P<0.001). Conclusion: The use of quercetin may be beneficial in the treatment of retinal I/R injury because of its antiapoptotic effect on the retinal layers, particularly in INL.en_US
dc.identifier.doi10.5935/0004-2749.20150026
dc.identifier.endpage104en_US
dc.identifier.issn0004-2749
dc.identifier.issn1678-2925
dc.identifier.issue2en_US
dc.identifier.pmid25945531en_US
dc.identifier.scopus2-s2.0-84942693943en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage100en_US
dc.identifier.urihttps://doi.org/10.5935/0004-2749.20150026
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23498
dc.identifier.volume78en_US
dc.identifier.wosWOS:000354050800009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherConsel Brasil Oftalmologiaen_US
dc.relation.ispartofArquivos Brasileiros De Oftalmologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectQuercetinen_US
dc.subjectRetina/Drug Effectsen_US
dc.subjectReperfusion Injuryen_US
dc.subjectApoptosisen_US
dc.subjectIschemiaen_US
dc.subjectDisease Modelsen_US
dc.subjectRatsen_US
dc.subjectOxidative Stressen_US
dc.subjectIschemia/Reperfusion Injuryen_US
dc.subjectGanglion-Cellsen_US
dc.subjectFlavonoidsen_US
dc.subjectAntioxidanten_US
dc.subjectPressureen_US
dc.subjectDamageen_US
dc.subjectMechanismsen_US
dc.subjectExpressionen_US
dc.subjectGlutamateen_US
dc.titleQuercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat modelen_US
dc.typeArticleen_US

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