Different doses of radiation on agar colony forming development in C6 glioma cells

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dc.authoridBilir, Ayhan/0009-0009-9399-5927
dc.authoridBiltekin, Burcu/0000-0002-8435-6797
dc.authorwosidGazi, Sevilcan Tuna/AAJ-1942-2020
dc.contributor.authorOzmen, Tolga
dc.contributor.authorOktem, Gulperi
dc.contributor.authorTuna, Sevilcan
dc.contributor.authorBiltekin, Burcu
dc.contributor.authorDenir, Secnur
dc.contributor.authorBilir, Ayhan
dc.date.accessioned2024-06-12T10:59:46Z
dc.date.available2024-06-12T10:59:46Z
dc.date.issued2007
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: Gliomas are relatively frequent in adults, and are among the most malignant primary brain tumors. Glioblastoma. multiforme, like many other tumors that exhibit radiation sensitivity in vitro, seems to be very resistant to radiation in vivo, thus suggesting that irradiation may not be a rate-limiting factor for malignant glioma tumor growth. In this study, we aimed to determine the optimal dose of radiation in C6 glioma colony forming assay, which is a valuable tool for antitumor treatment screening. Material and Methods: 10(5) cell/lamella colony forming cells were radiated with 200 cGy, 400 cGy, 800 cGy and 1600 cGy for 10 minutes. Radiosensitivity was measured systematically 24, 48, 72 and 96 hours after the radiation by three methods: soft-agar bilayer assay, thymidinE incorporation, and bromodeoxyuridine (BrdU) incorporation. Results: The soft-agar bilayer assay, which assessed the colony-forming units, showed that the number of colonies in the control group (609, 3 +/- 86.8) were decreased after 200 cGy (8.3 +/- 3.6) and 400 cGy (7.2 +/- 4.3). No colony was detected in 800 cGy and 1600 cGy irradiated cells [3H] Thymidine incorporation was more prominent with higher doses of radiation. BrdU incorporation revealed that even at low doses (200 cGy) of radiation there was a significant decrease of cell proliferation. On higher doses like 1600 cGy it was more prominent. Conclusion: Cell survival, doubling time, and cell phases are parameters of growth kinetics, and the results suggest that C6 glioma cells are radiosensitive and are virtually affected by all radiation doses in our experiment even 200 cGy at 24 hours. Besides, colony forming assay with thymidine labeling index, and BrdU labeling index may be used as new methods for determining radiotherapy doses in clinical applications.en_US
dc.identifier.endpage327en_US
dc.identifier.issn1300-0292
dc.identifier.issue3en_US
dc.identifier.startpage321en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20569
dc.identifier.volume27en_US
dc.identifier.wosWOS:000254582600002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDose Fractionationen_US
dc.subjectGliomaen_US
dc.subjectBreast-Carcinomaen_US
dc.subjectMalignant Gliomaen_US
dc.subjectIn-Vitroen_US
dc.subjectCanceren_US
dc.subjectSurvivalen_US
dc.subjectRadiochemotherapyen_US
dc.subjectSensitivityen_US
dc.subjectAdultsen_US
dc.subjectLineen_US
dc.subjectVivoen_US
dc.titleDifferent doses of radiation on agar colony forming development in C6 glioma cellsen_US
dc.typeArticleen_US

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