Esculetin Inhibits the Survival of Human Prostate Cancer Cells by Inducing Apoptosis and Arresting the Cell Cycle

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dc.authorwosidOZDEMIR, Mehmet Deniz/P-3283-2017
dc.contributor.authorTurkekul, Kader
dc.contributor.authorColpan, R. Dilsu
dc.contributor.authorBaykul, Talha
dc.contributor.authorOzdemir, Mehmet D.
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T11:01:30Z
dc.date.available2024-06-12T11:01:30Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Prostate cancer (PCa) is one of the most important causes of death in men and thus new therapeutic approaches are needed. In this study, antiproliferative and anti-migration properties of a coumarin derivative esculetin were evaluated. Methods: Human PCa cell lines PC3, DU145, and LNCaP were treated with various concentrations of esculetin for 24 to 72 hours, and cell viability was determined by the MTT test. Cell cycle and apoptosis were analyzed by using cell-based cytometer. Gene expression levels were assessed by reverse transcription and quantitative real-time PCR, cell migration was determined by the wound healing assay. The protein expression was measured by Western blotting. Results: Esculetin inhibited cell proliferation in a dose- and time-dependent manner. Cell migration was inhibited by esculetin treatment. Administration of esculetin significantly reduced the cells survival, induced apoptosis and caused the G1 phase cell cycle arrest shown by image-based cytometer. The induced expression of cytochrome c, p53, p21 and p27, and down-regulated CDK2 and CDK4 may be the underlying molecular mechanisms of esculetin effect. Esculetin suppressed phosphorylation of Akt and enhanced protein expression of tumor-suppressor phosphatase and tensin homologue. Conclusions: Our findings showed that the coumarin derivative esculetin could be used in the management of PCa. However, further in vivo research is needed.en_US
dc.identifier.doi10.15430/JCP.2018.23.1.10
dc.identifier.endpage17en_US
dc.identifier.issn2288-3649
dc.identifier.issn2288-3657
dc.identifier.issue1en_US
dc.identifier.pmid29629344en_US
dc.identifier.startpage10en_US
dc.identifier.urihttps://doi.org/10.15430/JCP.2018.23.1.10
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20904
dc.identifier.volume23en_US
dc.identifier.wosWOS:000477674500002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Soc Cancer Preventionen_US
dc.relation.ispartofJournal Of Cancer Preventionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEsculetinen_US
dc.subjectApoptosisen_US
dc.subjectCell Cycleen_US
dc.subjectProstate Canceren_US
dc.subjectCancer Preventionen_US
dc.subjectSignaling Pathwayen_US
dc.subjectPtenen_US
dc.subjectProliferationen_US
dc.subjectProgressionen_US
dc.subjectActivationen_US
dc.titleEsculetin Inhibits the Survival of Human Prostate Cancer Cells by Inducing Apoptosis and Arresting the Cell Cycleen_US
dc.typeArticleen_US

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