Identification of mycobacteria species by molecular methods

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dc.contributor.authorKunduracilar, Hakan
dc.date.accessioned2024-06-12T11:03:23Z
dc.date.available2024-06-12T11:03:23Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, mycobacteria, which were previously identified as Mycobacterium tuberculosis complex (MTC), and mycobacteria other than tuberculosis (MOTT) with cord factor and the p-nitro-alpha-acetyl-amino-beta-hydroxypropiophenone (NAP) test were reanalysed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis method in order to confirm the identification, and at the same time, species accepted as MOTT were identified. Although the results of the NAP test were obtained within 3-5 days, the PCR-RFLP results were obtained in 1 day. Ten species identified as MTC with the NAP test and cord factor were confirmed with the PCR-RFLP method. Fourteen species accepted as MOTT were identified as Mycobacterium species with the evaluation of the bands observed after the restriction of PCR product with the PCR-RFLP method. These were as follows: three species Mycobacterium intracellulare type I, two species Mycobacterium phlei, two species Mycobacterium kansasii, one species Mycobacterium fortuitum type I, one species Mycobacterium gordonae type I, one species Mycobacterium abscessus type I, one species Mycobacterium scrofulaceum, one species Mycobacterium szulgai type I, one species Mycobacterium avium type II, and one species Mycobacterium terrae. Hence, the results of both the cord factor and the NAP test were confirmed with the molecular method, and at the same time, mycobacteria species identification was made by determining the fastest, easiest, and the most accurate result-giving method. Because PCR-RFLP is a very rapid method that provides exact identification of mycobacteria species, it can be performed in routine procedures.en_US
dc.identifier.doi10.1111/iwj.13238
dc.identifier.endpage250en_US
dc.identifier.issn1742-4801
dc.identifier.issn1742-481X
dc.identifier.issue2en_US
dc.identifier.pmid31863568en_US
dc.identifier.scopus2-s2.0-85076915673en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage245en_US
dc.identifier.urihttps://doi.org/10.1111/iwj.13238
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21636
dc.identifier.volume17en_US
dc.identifier.wosWOS:000503614500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofInternational Wound Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHsp65en_US
dc.subjectMycobacteriaen_US
dc.subjectPCR-RFLPen_US
dc.subjectLength Polymorphism Analysisen_US
dc.subjectPresumptive Identificationen_US
dc.subjectRapid Identificationen_US
dc.subjectCord Formationen_US
dc.subjectHsp65 Geneen_US
dc.subjectTuberculosisen_US
dc.subjectComplexen_US
dc.subjectLevelen_US
dc.titleIdentification of mycobacteria species by molecular methodsen_US
dc.typeArticleen_US

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