Neferine inhibits proliferation and migration of human prostate cancer stem cells through p38 MAPK/JNK activation

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dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.contributor.authorErdogan, Suat
dc.contributor.authorTurkekul, Kader
dc.date.accessioned2024-06-12T10:51:12Z
dc.date.available2024-06-12T10:51:12Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractCancer stem cells (CSCs) are one of the significant causes of cancer treatment failure and metastasis, as they have significant chemo-and radio-resistance leading to tumor recurrence. Here we investigated the possible anticancer properties of neferine, a natural alkaloid, on human prostate cancer (PCa) cells and their stem cells. CD44(+) CSCs were isolated from androgen-insensitive PC3 cells by magnetic-activated cell sorting system (MACS). Neferine dose-and time-dependently inhibited the viability of PC3 and CSCs as well as androgen-sensitive LNCaP cells through inducing apoptosis and cell cycle arrest at G1 phase. Neferine was shown to downregulate the expression of Bcl-2 and CDK4, and upregulate caspase 3, clePARP, p21, p27, and p53. The treatment significantly inhibits the migration of CSCs. Neferine induces JNK and p38 MAPK phosphorylation, and downregulates PI3K and NF-& x138;beta signaling. In conclusion, neferine may have a therapeutic effect inhibiting the PCa cell proliferation as well as by eliminating CSCs. Practical applications Neferine is an alkaloid found in the seed embryo of Nelumbo nucifera and has recently been shown to have anticancer effects on various human cancer cells. More than 90% of cancer-related deaths develop after metastasis, and CSCs are considered to be largely responsible for the cell migration and invasion. It has been shown that treatment of neferine kills not only PCa cells but also CSCs, and may contribute to the prevention of progression of PCa and metastasis by inhibiting cell proliferation and migration.en_US
dc.identifier.doi10.1111/jfbc.13253
dc.identifier.issn0145-8884
dc.identifier.issn1745-4514
dc.identifier.issue7en_US
dc.identifier.pmid32394497en_US
dc.identifier.scopus2-s2.0-85084486926en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1111/jfbc.13253
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18280
dc.identifier.volume44en_US
dc.identifier.wosWOS:000531492300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Hindawien_US
dc.relation.ispartofJournal Of Food Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlkaloiden_US
dc.subjectCancer Stem Cellsen_US
dc.subjectCD44en_US
dc.subjectGrowth Arresten_US
dc.subjectNeferineen_US
dc.subjectProstate Canceren_US
dc.subjectLotus Seed Embryoen_US
dc.subjectInduced Apoptosisen_US
dc.subjectDown-Regulationen_US
dc.subjectSurvivalen_US
dc.subjectGrowthen_US
dc.subjectRosen_US
dc.subjectMechanismsen_US
dc.subjectCisplatinen_US
dc.subjectAutophagyen_US
dc.subjectMetastasisen_US
dc.titleNeferine inhibits proliferation and migration of human prostate cancer stem cells through p38 MAPK/JNK activationen_US
dc.typeArticleen_US

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