Effects of caffeic acid phenethyl ester (CAPE) on membrane potential and intracellular calcium in human endothelial cells

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dc.authorwosidAydogdu, Nurettin/ABH-9224-2020
dc.contributor.authorBurgazli, M. Kamil
dc.contributor.authorAydogdu, N.
dc.contributor.authorRafiq, A.
dc.contributor.authorMericliler, M.
dc.contributor.authorChasan, R.
dc.contributor.authorErdogan, A.
dc.date.accessioned2024-06-12T10:55:28Z
dc.date.available2024-06-12T10:55:28Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBACKGROUNG AND OBJECTIVES: Caffeic acid phenethyl ester (CAPE) is an active component of the resin propolis obtained from beehives. Propolis has a long history of medicinal use and a number of studies have already reported on some of its pharmaceutical properties. This study aimed to explore the effects of CAPE on the cytosolic Ca2+ concentration, cell proliferation, membrane potential and NO levels in human endothelial cells. MATERIALS AND METHODS: Isolated human umbilical vein endothelial cells (HUVEC) were incubated with CAPE (1-100 mu M) at 37 degrees C for 48 hours. Cell proliferation was estimated by counting cell numbers with use of a Neubauer chamber. The effect of CAPE (1-100 mu M) on the membrane potential was measured with the fluorescence dye DIBAC4(3) whereas its effect on the cytosolic Ca2+ concentration was measured by use of the fluorescence probe Fluo-3 AM (Invitrogen, Leiden, Netherlands). NO production was assayed using the flourophore DAF similar to AM (Invitrogen, Leiden, Netherlands). Changes in fluorescence intensity was determined with the GENios plate reader (Genios, Tecan, Austria). RESULTS: A Dose-dependent hyperpolarization of the endothelial cell membrane was observed with CAPE stimulation. The initial increase in the intracellular Ca2+ concentration showed a subsequent decrease over time. CAPE stimulation also resulted in an increase in NO production; however, at higher doses a decrease in NO levels was observed. HUVEC proliferation was inhibited by CAPE. CONCLUSIONS: Here we report on the effect of CAPE stimulation on the cytosolic Ca2+ concentration, cell proliferation, membrane potential and NO production in HUVEC in a dose-dependent manner. These findings provide important insights into some potential key roles that both calcium and the membrane potential play in the CAPE activation of endothelial cells in a concentration-dependent manner.en_US
dc.identifier.endpage728en_US
dc.identifier.issn1128-3602
dc.identifier.issue6en_US
dc.identifier.pmid23609354en_US
dc.identifier.scopus2-s2.0-84878585529en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage720en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19439
dc.identifier.volume17en_US
dc.identifier.wosWOS:000325207900002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical And Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeic Acid Phenthyl Esteren_US
dc.subjectEndothelial Cellsen_US
dc.subjectCalciumen_US
dc.subjectMebrane Potentialen_US
dc.subjectNitric-Oxide Productionen_US
dc.subjectHeme Oxygenase-1en_US
dc.subjectApoptosisen_US
dc.subjectProliferationen_US
dc.subjectAngiogenesisen_US
dc.subjectConductanceen_US
dc.subjectSuppressionen_US
dc.subjectInductionen_US
dc.subjectPropolisen_US
dc.subjectChannelsen_US
dc.titleEffects of caffeic acid phenethyl ester (CAPE) on membrane potential and intracellular calcium in human endothelial cellsen_US
dc.typeArticleen_US

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