Stress-induced miRNAs isolated from wheat have a unique therapeutic potential in ultraviolet-stressed human keratinocyte cells

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dc.contributor.authorArda, Hayati
dc.contributor.authorDoganlar, Oguzhan
dc.date.accessioned2024-06-12T11:17:00Z
dc.date.available2024-06-12T11:17:00Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIncreasing evidence supports the existence of cross-kingdom gene regulation. However, the therapeutic potential of stress-specific plant miRNAs and their role in UV-related pathologies in human tissue remain largely unexplored. The aim of this study was to investigate the therapeutic potential and mechanisms of action of stress-induced miRNA cocktails (SI-WmiRs) from Einkorn wheat (Triticum monococcum L.) on human keratinocyte (HaCaT) cells exposed to a high dose of UV-B radiation. We used a biofactory approach and irradiated wheatgrass with UV-C for 240 min to obtain the specific SI-WmiRs that wheat produces to recover from UV stress. We followed the plant with molecular and biochemical analyses and extracted our SI-WmiRs at the most appropriate time (0 h and 6 h after UV-C application). Then, we applied the SI-WmiR cocktail to HaCaT cells exposed to high-dose of UV-B radiation. Our results show that UV-B radiation induced lipid peroxidation and DNA damage, as demonstrated by increased malondialdehyde (MDA) levels and changes in the RAPD band profile, respectively. UV stress also impaired IL6/JAK2/STAT3 signalling and activated the inflammatory mediators IL6 and TNF-alpha in HaCaT cells, leading to significant induction of apoptotic cell death. We found that SI-WmiR transfection prevents lipid peroxidation and oxidative stress-related DNA damage by increasing antioxidant (CuZn-SOD, Mn-SOD) and DNA repair (EXO1, SMUG1 and XRCC3) gene expression. In addition, SI-WmiRs regulated IL6/JAK2/STAT3 signalling by reducing JAK2 and STAT3 gene expression and phosphorylated protein levels compared to the control treatments. Moreover, SI-WmiRs inhibited pro-apoptotic BAX, Caspase 3 and Caspase 8 gene expression and protein levels to prevent apoptosis of UV-stressed HaCaT cells. Our results demonstrate that stress-induced wheat miRNAs produced using a biofactory approach have strong potential as a novel and effective alternative therapy for UV stress-related skin damage.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Trakya University [TUBAP-2019/209]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Trakya University; Project Number: TUBAP-2019/209.en_US
dc.identifier.doi10.1007/s11356-021-17039-8
dc.identifier.endpage17996en_US
dc.identifier.issn0944-1344
dc.identifier.issn1614-7499
dc.identifier.issue12en_US
dc.identifier.pmid34677776en_US
dc.identifier.scopus2-s2.0-85117716779en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage17977en_US
dc.identifier.urihttps://doi.org/10.1007/s11356-021-17039-8
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24537
dc.identifier.volume29en_US
dc.identifier.wosWOS:000710092200017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofEnvironmental Science And Pollution Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUV Stressen_US
dc.subjectMicrornaen_US
dc.subjectDNA Damageen_US
dc.subjectApoptosisen_US
dc.subjectEinkorn Wheaten_US
dc.subjectHacat Cellsen_US
dc.subjectCross-Kingdom Regulationen_US
dc.subjectOxidative Stressen_US
dc.subjectSignaling Pathwayen_US
dc.subjectDna-Damageen_US
dc.subjectMicrornasen_US
dc.subjectPlanten_US
dc.subjectRadiationen_US
dc.subjectApoptosisen_US
dc.subjectResponsesen_US
dc.subjectDeliveryen_US
dc.titleStress-induced miRNAs isolated from wheat have a unique therapeutic potential in ultraviolet-stressed human keratinocyte cellsen_US
dc.typeArticleen_US

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