Sıçanlarda morris su labirenti uzaysal öğrenme eğitimleri sırasında hipokampüste pp1 ve reelin genlerinin ekspresyonlarında meydana gelen değişikliklerin belirlenmesi
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Dosyalar
Tarih
2015
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Yayıncı
Trakya Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Hipokampüs uzaysal öğrenme ve bellek oluşumuyla ilgili bir merkezdir. Çeşitli genlerin bellek oluşumunu indüklediği veya inhibe ettiği düşünülmektedir. Çalışmamızda sıçanlarda uzaysal öğrenme ve bellek gelişiminde hipokampüste BDNF, reelin, PP1 genlerinin ekspresyonlarında meydana gelen günlük değişim incelenmesi amaçlanmıştır. Her birinde 5 erişkin erkek sıçan bulunan gruplar uzaysal öğrenme ve bellek eğitimi için her gün 4 kez olmak üzere 5 gün süreyle eğitilmiştir. Altıncı gün platform çıkarılmış ve bellek performansları incelenmiştir. Eğitimlerin tamamlanmasından sonra, ötanazi uygulanarak beyin dokuları çıkarılmıştır. Hipokampüs CA1 ve CA3 alanlarından alınan doku örneklerinde polimeraz zincir reaksiyonu analizleri yapılmıştır. Sonuçlarımızda, CA1 bölgesinde dördüncü günde BDNF ekspresyonunun kontrole göre istatistiksel anlamlı derecede (p<0,01) arttığını göstermiştir. Her iki bölgeden alınan doku örneklerinde reelin ve PP1 ekspresyonlarındaki değişimler ise istatistiksel anlamlılığa erişmemiştir. Sonuçlarımız BDNF’nin uzaysal öğrenme ve bellek sürecinde birinci günden itibaren rolünün olduğunu düşündürmektedir.
abstract
Hippocampus is a center related to spatial learning and memory formation. It has been thought that several genetic changes occur in the hippocampus in the process of learning and memory formation. In this study, it is aimed to investigate activity changes of PP1, BDNF, reelin genes in hippocampus during learning and memory formation after successive trainings in rats. The groups of animals [n=5] were given 4 trials per day for 5 consecutive days to locate a hidden platform. On the sixth day the platform was removed and the animals were swum. Learning and memory functions of the animals were evaluated based on their performances in these tests. After memory tests, the rats that completed training were euthanized and their brain tissues were removed. Tissue samples were taken at hippocampal CA1 and CA3 zones. PP1, BDNF and Reelin gene expressions in the taken tissue samples were analyzed by polymerase chain reaction. Our results show that in the CA1 region, BDNF gene expression increased in a statistically significant degree (p<0,01) on the fourth day. In tissue samples taken from both regions, there were no statistically significant differences in reelin and PP1 gene expressions. Our results suggest that BDNF has a role in spatial learning and memory processes starting from the first day.
abstract
Hippocampus is a center related to spatial learning and memory formation. It has been thought that several genetic changes occur in the hippocampus in the process of learning and memory formation. In this study, it is aimed to investigate activity changes of PP1, BDNF, reelin genes in hippocampus during learning and memory formation after successive trainings in rats. The groups of animals [n=5] were given 4 trials per day for 5 consecutive days to locate a hidden platform. On the sixth day the platform was removed and the animals were swum. Learning and memory functions of the animals were evaluated based on their performances in these tests. After memory tests, the rats that completed training were euthanized and their brain tissues were removed. Tissue samples were taken at hippocampal CA1 and CA3 zones. PP1, BDNF and Reelin gene expressions in the taken tissue samples were analyzed by polymerase chain reaction. Our results show that in the CA1 region, BDNF gene expression increased in a statistically significant degree (p<0,01) on the fourth day. In tissue samples taken from both regions, there were no statistically significant differences in reelin and PP1 gene expressions. Our results suggest that BDNF has a role in spatial learning and memory processes starting from the first day.
Açıklama
Yüksek Lisans Tezi
Anahtar Kelimeler
BDNF, Bellek, Uzaysal Öğrenme, PP1, Reelin, Spatial Learning, Memory
