Deneysel böbrek iskemi/reperfüzyon hasarında Kisspeptinin rolünün incelenmesi
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Dosyalar
Tarih
2018
Yazarlar
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Yayıncı
Trakya Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
Kisspeptin, KİSS1 geni tarafından kodlanan, böbrek tübül, damar ve toplayıcı kanal hücrelerinde yapısal olarak bulunan, tümör metastazını baskılamada, üremenin düzenlenmesinde ve vazokonstriktör olarak önemli rol oynayan aktif bir peptidtir. Çalışmamızda deneysel böbrek I/R hasarında kisspeptinin böbrek fonksiyonları, ANG II, ACE ve aldosteron ile ilişkilerini ve böbrek fizyopatolojisindeki rolünü araştırmayı amaçladık. Çalışmamızda 300-350 gr ağırlığında 16 adet Spraque-Dawley erkek sıçan Kontrol ve İ/R grubu olmak üzere 2 gruba ayrıldı. Grup 1 sıçanların böbrek damarları diseksiyon ile ayrıldı ve insizyon 60 dakika sonra kapatıldı. Grup 2'deki renal damarlar diseksiyon ile ayrıldı ve 60 dakika iskemiden 48 saat sonra mikrovasküler klemp reperfüze edildi. 24 saat idrar toplandıktan sonra sıçanlar anestezi altında sakrifiye edildi. Kan ve böbrek dokusu örnekleri toplandı. Çalışmamızda Kontrol grubuna göre İ/R grubunda serum AST, ALT, üre, kreatinin, ACE (p<0.01), idrar Kim-1 düzeylerinde artma (p<0.05), fraksiyonel sodyum atılımında, kreatinin klirensi, serum aldosteron ve ANG II düzeylerinde azalma görüldü (p<0.05). Serum kispeptin düzeylerinde anlamlı bir değişiklik olmamasına rağmen, böbrek kisspeptin düzeylerinin azaldığı ve idrar kisspeptin düzeylerinin arttığı saptanmıştır (p<0.05). Sonuç olarak bulgularımız, kisspeptinin akut böbrek hasarı ve böbrek fonksiyonlarının fizyopatolojisi ile ilişkili olabileceğini göstermiştir. Kisspeptinin bu ilişkisinin mekanizmasının ayrıntılı olarak araştırılması gerektiğini düşünüyoruz.
Kisspeptin is an active peptide encoded by the KISS1 gene that is structurally found in the kidney tubule, vascular and collecting duct cells, plays an important role in suppressing tumor metastasis, regulating the uremia, and acting as a vasoconstrictor. İn our study we aimed to investigate the role of kisspeptin in kidney I/R injury, kidney function, ANG II, ACE and Aldosterone, and its role in renal pathophysiology. In our study, 16 groups of Spraque-Dawley male rats weighing 300-350 gr and 2 groups of I/R groups were separated. The kidney vessels of group 1 rats were separated by dissection and the incision was closed after 60 minutes. Renal vessels in group 2 were separated by dissection and microvascular clamp was reperfused 48 hours after 60 minutes of ischemia. After 24 hours of urine collection, the rats were sacrificed under anesthesia. Blood and kidney tissue samples were collected. Serum AST, ALT, urea, creatinine, ACE (p<0.01), urine Kim-1 levels (p<0.05), fractional sodium excretion, creatinine clearance, serum aldosterone and ANG II levels decreased in the I/R group according to the control group (p<0.05). Although there was no significant change in serum kisspeptin levels, kidney kisspeptin levels decreased and urine kisspeptin levels increased (p <0.05).In conclusion, our findings have shown that kisspeptin may be associated with acute renal damage and renal function physiopathology. We think that the mechanism of this association of the kisspeptin should be investigated in detail.
Kisspeptin is an active peptide encoded by the KISS1 gene that is structurally found in the kidney tubule, vascular and collecting duct cells, plays an important role in suppressing tumor metastasis, regulating the uremia, and acting as a vasoconstrictor. İn our study we aimed to investigate the role of kisspeptin in kidney I/R injury, kidney function, ANG II, ACE and Aldosterone, and its role in renal pathophysiology. In our study, 16 groups of Spraque-Dawley male rats weighing 300-350 gr and 2 groups of I/R groups were separated. The kidney vessels of group 1 rats were separated by dissection and the incision was closed after 60 minutes. Renal vessels in group 2 were separated by dissection and microvascular clamp was reperfused 48 hours after 60 minutes of ischemia. After 24 hours of urine collection, the rats were sacrificed under anesthesia. Blood and kidney tissue samples were collected. Serum AST, ALT, urea, creatinine, ACE (p<0.01), urine Kim-1 levels (p<0.05), fractional sodium excretion, creatinine clearance, serum aldosterone and ANG II levels decreased in the I/R group according to the control group (p<0.05). Although there was no significant change in serum kisspeptin levels, kidney kisspeptin levels decreased and urine kisspeptin levels increased (p <0.05).In conclusion, our findings have shown that kisspeptin may be associated with acute renal damage and renal function physiopathology. We think that the mechanism of this association of the kisspeptin should be investigated in detail.
Açıklama
Anahtar Kelimeler
Kisspeptin, Akut Böbrek Hasarı, İskemi/reperfüzyon, Kisspeptin, Acute Kidney Injury, İschemia/reperfusion
