Characterization of pncA mutations of pyrazinamide-resistant Mycobacterium tuberculosis in Turkey

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dc.contributor.authorYuksel, Pelin
dc.contributor.authorTansel, Ozlem
dc.date.accessioned2024-06-12T11:08:55Z
dc.date.available2024-06-12T11:08:55Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractMutations in the pyrazinamidase (PZase) gene (pncA) are considered the major mechanism of pyrazinamide (PZA) resistance in Mycobacterium tuberculosis. The aim of this study was designed to determine pncA mutations among ten PZA resistant and two PZA susceptible M. tuberculosis strains from Turkey and also to compare the PZase activity of them with the genotype. All isolates were identified by BACTEC NAP (P-nitro-alpha-acetylamino-beta-hydroxy-propiophenone)test and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method. Drug sensitivity tests were performed by BACTEC system. PncA mutations were detected by DNA sequence analysis. No mutation was detected in two PZA susceptible and three out of tell PZA resistant strains. While, two of the PZA resistant strains had mutations in the same region (Gly24Asp), two of the PZA resistant strains had mutations in different regions (Thr160Lys), (His51Pro). Three of the PZA resistant strains had frameshift as a 167 bp deletion at nucleotide position 102. As a result, we detected two new mutations and a frameshift which may be responsible for PZA resistance in this study different from the other studies which previously 51st codon mutation was reported.en_US
dc.description.sponsorshipGovernment Planning Organization [2002KI20500]en_US
dc.description.sponsorshipThis study was supported by Government Planning Organization (Project No: 2002KI20500). Project a. title is 'Determination of mutation against major anti-tuberculosis drugs in M. tuberculosis bacilli by DNA sequencing analysis in Turkey'. The authors thank Prof. Dr. Ahmet Sani for technical support.en_US
dc.identifier.endpage158en_US
dc.identifier.issn1121-7138
dc.identifier.issue2en_US
dc.identifier.pmid19579692en_US
dc.identifier.scopus2-s2.0-67649935699en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage153en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22603
dc.identifier.volume32en_US
dc.identifier.wosWOS:000266757500005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherEdizioni Int Srlen_US
dc.relation.ispartofNew Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMycobacterium Tuberculosisen_US
dc.subjectDrug Resistanceen_US
dc.subjectPyrazinamideen_US
dc.subjectPnca Mutationsen_US
dc.subjectSusceptibilityen_US
dc.subjectIdentificationen_US
dc.subjectStrainsen_US
dc.subjectRegionen_US
dc.subjectBrazilen_US
dc.subjectRpoben_US
dc.titleCharacterization of pncA mutations of pyrazinamide-resistant Mycobacterium tuberculosis in Turkeyen_US
dc.typeArticleen_US

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