Puerarin Protects from Methotrexate Induced Hepatotoxicity in AML-12 Cells

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dc.authoridOltulu, Cagatay/0000-0002-6051-3479;
dc.authorwosidOltulu, Cagatay/V-1823-2018
dc.authorwosidYakut, Zatiye Ayça Çevikelli/ACP-2487-2022
dc.contributor.authorAkinci, Melek
dc.contributor.authorOltulu, Cagatay
dc.contributor.authorBakar, Elvan
dc.contributor.authorCevikelli Yakut, Zatiye Ayca
dc.date.accessioned2024-06-12T11:08:26Z
dc.date.available2024-06-12T11:08:26Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAim: The purpose of this study was to look into the effects of puerarin (PR) on methotrexate (MTX)-induced hepatotoxicity in vitro. Materials and Methods: We designed our research with four groups in the AML-12 cell line: control, PR, MTX, and PR+MTX groups. Administered concentration levels to the cell lines were determined with the MTT test. To investigate oxidative stress, the expression levels of glutathione, superoxide dismutase, and catalase were determined with quantitative real-time-polymerase chain reaction (qRT-PCR) analysis. To evaluate the role of apoptosis pathways in MTX induced hepatotoxicity and the hepatoprotective effects of PR, gene expressions of caspase 3 (Cas-3), Cas-9, apoptotic protease activating factor-1, Bcl-2, Bax, p53, second mitochondria-derived activator of caspase/direct inhibitor of apoptosis-binding protein (smac/DIABLO), topoisomerase (Top) I, and Top II were investigated with qRT-PCR. Results: MTX impaired the antioxidant defense through SOD but elevated the expression of catalase and glutathione due to an increase in free radicals. In the PR+MTX group, SOD expression increased and catalase and glutathione expression decreased compared to the MTX group. Cas-9, Apaf-1, and Top I gene expression levels were reduced in group PR. In the group of PR+MTX, PR application increased the expression of Bax, p53, and smac/DIABLO while decreasing the expression of Bcl-2, which resulted in the elimination of damaged structures by apoptosis. Conclusion: PR alleviated the hepatotoxicity caused by MTX with its antioxidant effects and positive effects on apoptosis pathways. However, different dose studies are needed because PR could not prevent double-strand damage in DNA due to MTX and there is an increase in Top I expression in the PR group.en_US
dc.description.sponsorshipTrakya University Scientific Research Projects Committee [2018/274]en_US
dc.description.sponsorshipFinancial Disclosure: This study was funded by Trakya University Scientific Research Projects Committee with the grant number 2018/274.en_US
dc.identifier.doi10.4274/nkmj.galenos.2023.27147
dc.identifier.endpage201en_US
dc.identifier.issn2587-0262
dc.identifier.issue3en_US
dc.identifier.startpage193en_US
dc.identifier.urihttps://doi.org/10.4274/nkmj.galenos.2023.27147
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22436
dc.identifier.volume11en_US
dc.identifier.wosWOS:001187553300005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofNamik Kemal Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMethotrexateen_US
dc.subjectPuerarinen_US
dc.subjectHepatoprotectiveen_US
dc.subjectOxidative Stressen_US
dc.subjectApoptosisen_US
dc.subjectOxidative Stressen_US
dc.subjectApoptosisen_US
dc.subjectExpressionen_US
dc.subjectInflammationen_US
dc.subjectDamageen_US
dc.subjectLiveren_US
dc.titlePuerarin Protects from Methotrexate Induced Hepatotoxicity in AML-12 Cellsen_US
dc.typeArticleen_US

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