Antioxidant and Tyrosinase Inhibitory Activities of Flavonoids from Trifolium nigrescens Subsp petrisavi
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Chemical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Trifolium nigrescens was researched for its chemical constituents for the first time. Bioassay-guided isolation of the EtOAc extract from the leaves of T. nigrescens resulted in the purification of a new biflavone, 4',5,5 '',7,7 ''-pentahydroxy-3',3'-dimethoxy-3-O-beta-D-glucosyl-3 '',4'-O-biflavone (1) along with eleven known compounds consisting of three phenolics (2-4), and eight flavonoid glycosides (5-12). Their structures were determined by extensive ID and 2D NMR, and MS data analyses. The isolated compounds were evaluated for their antioxidant activity and inhibitory activity on mushroom tyrosinase. Highly potent inhibitions Were found by compounds 7 (IC50 = 0.38 mM), 8 (IC50 = 0.19 mM), and 12 (IC50 = 0.26 mM) when compared with standard tyrosinase inhibitors kojic acid (IC50 = 0.67 mM) and L-mimosine (IC50 = 0.64 mM). The antioxidative effect of the isolated compounds and the extracts were determined by using, beta-carotene-linoleic acid, DPPH center dot scavenging, ABTS(+center dot) scavenging, superoxide scavenging, and CUPRAC assays. The experimental findings indicated that all the compounds demonstrated activity in all antioxidant activity tests employed except for the compounds 2-6. Compounds 2-6 showed moderate activity only in ABTS(+center dot) scavenging assay. The new compound 1 exhibited better activity than standard alpha-tocopherol in DPPH center dot scavenging, and ABTS(+center dot) scavenging assays. The results show that T. nigrescens can be regarded as a potential source of antioxidant compounds and tyrosinase inhibitors of significance in both the pharmaceutical and food industries.
Açıklama
Anahtar Kelimeler
Fabaceae, T. Nigrescens Subsp Petrisavi, Flavonoids, Phenolic Compounds, Tyrosinase Inhibitory Activity, Antioxidant Activity, Extracts
Kaynak
Journal Of Agricultural And Food Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
61
Sayı
51
