Effects of permissible maximum-contamination levels of VOC mixture in water on total DNA, antioxidant gene expression, and sequences of ribosomal DNA of Drosophila melanogaster

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidZhang, Bingbo/A-6818-2013
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorTabakcioglu, Kiymet
dc.date.accessioned2024-06-12T11:17:12Z
dc.date.available2024-06-12T11:17:12Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, we aimed to investigate the mutagenic and carcinogenic potential of a volatile organic compound (VOC) mixture with references to the response of D. melanogaster using selected antioxidant gene expressions, RAPD assay and base-pair change of ribosomal 18S, and the internal transcribed spacer, ITS2 rDNA gene sequences. For this purpose, Drosophila melanogaster Oregon R, reared under controlled conditions on artificial diets, were treated with the mixture of thirteen VOCs, which are commonly found in water in concentrations of 10, 20, 50, and 75 ppb for 1 and 5 days. In the random amplified polymorphic DNA (RAPD) assay, band changes were clearly detected, especially at the 50 and 75 ppb exposure levels, for both treatment periods, and the band profiles exhibited clear differences between the treated and untreated flies with changes in band intensity and the loss/appearance of bands. Quantitative real-time PCR (qRT-PCR) analysis of Mn-superoxide dismutase (Mn-SOD), catalase (CAT) and glutathione-synthetase (GS) expressions demonstrated that these markers responded significantly to VOC-induced oxidative stress. Whilst CAT gene expressions increased linearly with increasing concentrations of VOCs and treatment times, the 50- and 75-ppb treatments caused decreases in GS expressions compared to the control at 5 days. Treatment with VOCs at both exposure times, especially in high doses, caused gene mutation of the 18S and the ITS2 ribosomal DNA. According to this research, we thought that the permissible maximum-contamination level of VOCs can cause genotoxic effect especially when mixed.en_US
dc.description.sponsorshipT.R. State Planning Organization [2011 K120390]; Trakya University Research Project Foundation, Edirne/Turkey [TUBAP-2013/117]en_US
dc.description.sponsorshipThe author is grateful to Prof. Dr. Yener Yoruk (Rector of Trakya University, Edirne/Turkey) and the Technology Research and Application Centre (TUTAGEM) which is funded by the T.R. State Planning Organization (Project Number: 2011 K120390) for providing laboratory equipment. This study was supported by the Trakya University Research Project Foundation (Project Number: TUBAP-2013/117), Edirne/Turkey.en_US
dc.identifier.doi10.1007/s11356-015-4741-y
dc.identifier.endpage15620en_US
dc.identifier.issn0944-1344
dc.identifier.issn1614-7499
dc.identifier.issue20en_US
dc.identifier.pmid26018283en_US
dc.identifier.scopus2-s2.0-84945465771en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage15610en_US
dc.identifier.urihttps://doi.org/10.1007/s11356-015-4741-y
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24597
dc.identifier.volume22en_US
dc.identifier.wosWOS:000363966900036en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofEnvironmental Science And Pollution Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVOC Mixtureen_US
dc.subjectAntioxidant-Enzyme Genesen_US
dc.subject18S And ITS2 Rdnaen_US
dc.subjectGenotoxicityen_US
dc.subjectDrosophila Melanogasteren_US
dc.subjectVolatile Organic-Compoundsen_US
dc.subjectCerebellar Granule Cellsen_US
dc.subjectEnvironmental-Changesen_US
dc.subjectExposureen_US
dc.subjectBenzeneen_US
dc.subjectTolueneen_US
dc.subjectStressen_US
dc.subjectGenotoxicityen_US
dc.subjectPollutionen_US
dc.subjectWorkersen_US
dc.titleEffects of permissible maximum-contamination levels of VOC mixture in water on total DNA, antioxidant gene expression, and sequences of ribosomal DNA of Drosophila melanogasteren_US
dc.typeArticleen_US

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