Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
General Physiol And Biophysics
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Paraoxonase-1 (PON1) and paraoxonase-3 (PON3) are anti-atherosclerotic enzymes, synthesized primarily in liver and bound to HDL in circulation. The aim of the present study was to investigate the effects of therapeutic doses of lipoic acid on PON1 and PON3 protein levels, mRNA expression and arylesterase activity in liver. We treated HepG2 cells with 10, 40 and 200 mu M lipoic acid for 72 h. Cell viability was evaluated by 3-(4,5-dimethy1-2-thiazoly1)-2,5-dipheny1-2Htetrazolium bromide assay. PON1 and PON3 protein levels were measured by Western blotting, their mRNA expression was measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. 200 mu M lipoic acid caused a significant increase on PON1 and PON3 protein levels and arylesterase activity as compared with control, 10 mu M and 40 mu M lipoic acid treated cells. 200 mu M lipoic acid also caused a significant decrease on PON1 mRNA expression whereas on a significant increase PON3 mRNA expression as compared with control, 10 mu M and 40 mu M lipoic acid-treated cells. Our study showed that although lip oic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses.
Açıklama
Anahtar Kelimeler
Lipoic Acid, Paraoxonase-1, Paraoxonase-3, Arylesterase, Liver, Low-Density-Lipoprotein, Biological-Activity, Oxidative Stress, Gene-Expression, Atherosclerosis, Antioxidant, Rats, Family, Enzyme, Hdl
Kaynak
General Physiology And Biophysics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
36
Sayı
4
