Characterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell lines

Basit Öğe Kaydı
dc.authoridDinc, Bircan/0000-0002-9717-6410
dc.authoridunlu, ayhan/0000-0001-6033-7148
dc.authoridBektas, Muhammet/0000-0002-4438-1664
dc.authorwosidDinc, Bircan/ADG-4955-2022
dc.authorwosidunlu, ayhan/Q-1843-2016
dc.contributor.authorDinc, Bircan
dc.contributor.authorUnlu, Ayhan
dc.contributor.authorBektas, Muhammet
dc.date.accessioned2024-06-12T11:08:50Z
dc.date.available2024-06-12T11:08:50Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractMulti-walled carbon nanotubes (MWNTs) are suitable for delivering large biomolecules with lower cytotoxicity values and low prime cost. Surface modifications of MWNTs affect interaction with cells and proteins. Oxidation with strong acids decreases cytotoxicity of CNTs and increases protein-loading capacity. Here, after oxidation, TEM images revealed more aligned structure and carboxylated groups at the surface which decreases toxicity. Functionalized MWNTs showed more gradual degradation than the pristine MWNTs and mass loss increased by 2% in the same temperature range. Raman spectroscopy corrected graphitic structure with characteristic D and G bands at 1330 and 1579 cm(-1) and increased intensity after oxidation. FTIR spectroscopy peaks at 1443 cm(-1), 1560, 1640 cm(-1), 2100-2200 cm(-1) and 3426 cm(-1) are ascribed to C-O-C vibrational stretch, C=C bonds, vibration of C equivalent to C bonds and stretch of hydroxyl groups, respectively. The sonication-driven dispersion of in phosphate-buffered saline, distilled water and cell culture medium were detected by UV-vis-NIR spectroscopy, water-dispersed functionalized MWNTs revealed the highest absorbance value. Cytotoxicity of MWNTs was investigated before and after functionalization in breast cancer (MDA-MB-231) and human vein endothelial (HUVEC) cells. Relatively low-toxicity results were obtained in functionalized MWNTs and cellular uptake of MWNTs were corrected with fluorescent imaging of cells and cell lysates. Protein-loading capacity of fsMWNTs (functionalized short-length multi-walled carbon nanotubes) was evaluated by using bovine serum albumin (BSA) and with an equal amount of fsMWNTs and BSA; 36% binding yield was obtained. Protein corona after covalent functionalization potentially lowered cytotoxicity up to 6%.en_US
dc.description.sponsorshipResearch Fund of the Istanbul University [29833]en_US
dc.description.sponsorshipThis work was supported by the Research Fund of the Istanbul University (Grant 29833).en_US
dc.identifier.doi10.1007/s42823-019-00081-5
dc.identifier.endpage153en_US
dc.identifier.issn1976-4251
dc.identifier.issn2233-4998
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85072013180en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage143en_US
dc.identifier.urihttps://doi.org/10.1007/s42823-019-00081-5
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22586
dc.identifier.volume30en_US
dc.identifier.wosWOS:000521185200004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Japan Kken_US
dc.relation.ispartofCarbon Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMulti-Walled Carbon Nanotubesen_US
dc.subjectCharacterizationen_US
dc.subjectBovine Serum Albuminen_US
dc.subjectCytotoxicityen_US
dc.subjectSolubilityen_US
dc.subjectBovine Serum-Albuminen_US
dc.subjectRaman-Spectroscopyen_US
dc.subjectIn-Vitroen_US
dc.subjectFunctionalizationen_US
dc.subjectToxicityen_US
dc.subjectTranslocationen_US
dc.subjectNanomaterialsen_US
dc.subjectDeliveryen_US
dc.subjectGeneen_US
dc.titleCharacterization of short-length multi-walled carbon nanotubes and cytotoxicity on MDA-MB-231 and HUVEC cell linesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu