Protective Effect of Combined Caffeic Acid Phenethyl Ester and Bevacizumab Against Hydrogen Peroxide-Induced Oxidative Stress in Human RPE Cells

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dc.authorwosidAyaz, Lokman/K-6716-2013
dc.contributor.authorDinc, Erdem
dc.contributor.authorAyaz, Lokman
dc.contributor.authorKurt, Akif Hakan
dc.date.accessioned2024-06-12T10:50:56Z
dc.date.available2024-06-12T10:50:56Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: This study aimed to evaluate the protective effects of caffeic acid phenethyl ester (CAPE) and combined CAPE-bevacizumab against oxidative stress induced by hydrogen peroxide (H2O2) in human retinal pigment epithelium. Methods: ARPE-19 cells were pretreated with 5, 10, and 30 mu M CAPE alone and in combination with bevacizumab for 3 h, then exposed to H2O2 for 16 h. Cell viability was evaluated with the 3-(4,5dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Vascular endothelial growth factor (VEGF) protein levels in the medium were measured using a human VEGF ELISA kit. Total antioxidant status (TAS) and total oxidant status (TOS) were measured in ARPE-19 cells using the test kit from Rel Assay. Expression levels of VEGF, Bax, Bcl-2, cytochrome c, apoptotic protease activating factor-1 (apaf-1), and caspase-3 were determined using reverse transcription polymerase chain reaction. Results: Pretreatment of ARPE-19 cells with 30 mu M CAPE and combined CAPE-bevacizumab reduced H2O2 mediated cell death. H2O2-induced oxidative stress increased TOS and VEGF production, which was significantly inhibited by CAPE and the CAPE-bevacizumab combination. VEGF, Bax, cytochrome c, apaf-1, and caspase-3 gene expressions were significantly decreased in cells pretreated with 5, 10, and 30 mu M CAPE and combined CAPE-bevacizumab compared to the H2O2 group. In addition, Bcl-2 expression was significantly increased in both the CAPE and CAPE-bevacizumab combination groups compared to the H2O2 group. Conclusions: CAPE has a protective effect on ARPE-19 cells against oxidative stress, and VEGF protein level and expression can be decreased by incubation with different concentrations of CAPE. These results demonstrate that CAPE suppresses the mitochondria-mediated apoptosis in ARPE-19 cells under oxidative stress. In addition, the use of CAPE in combination with bevacizumab has an additive effect.en_US
dc.description.sponsorshipTrakya University Scientific Research Fund [TUBAP 2015/232]en_US
dc.description.sponsorshipThis study was supported by Trakya University Scientific Research Fund (Grant No: TUBAP 2015/232).en_US
dc.identifier.doi10.1080/02713683.2017.1368085
dc.identifier.endpage1666en_US
dc.identifier.issn0271-3683
dc.identifier.issn1460-2202
dc.identifier.issue12en_US
dc.identifier.pmid28937872en_US
dc.identifier.scopus2-s2.0-85029899608en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1659en_US
dc.identifier.urihttps://doi.org/10.1080/02713683.2017.1368085
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18183
dc.identifier.volume42en_US
dc.identifier.wosWOS:000422853400015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofCurrent Eye Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAge Related Macular Degenerationen_US
dc.subjectBevacizumaben_US
dc.subjectCaffeic Acid Phenethyl Esteren_US
dc.subjectCAPEen_US
dc.subjectOxidative Stressen_US
dc.subjectRetinal Pigment Epitheliumen_US
dc.subjectEndothelial Growth-Factoren_US
dc.subjectPigment Epithelial-Cellsen_US
dc.subjectMacular Degenerationen_US
dc.subjectCytochrome-Cen_US
dc.subjectApoptosisen_US
dc.subjectBaxen_US
dc.subjectExpressionen_US
dc.subjectReleaseen_US
dc.subjectRatsen_US
dc.subjectNeovascularizationen_US
dc.titleProtective Effect of Combined Caffeic Acid Phenethyl Ester and Bevacizumab Against Hydrogen Peroxide-Induced Oxidative Stress in Human RPE Cellsen_US
dc.typeArticleen_US

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