Low concentration of oleic acid exacerbates LPS-induced cell death and inflammation in human alveolar epithelial cells

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dc.contributor.authorKucukgul, Altug
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T10:52:27Z
dc.date.available2024-06-12T10:52:27Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: The current study aimed to investigate in vitro effects of oleic acid on lipopolysaccharide (LPS)-induced acute lung injury in the human lung epithelial cells (A549). Materials and Methods: The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests. Selected gene expression levels were analyzed by Real-Time Quantitative-Polymerase Chain Reaction (RT-qPCR). Results: 24hours of LPS (100ng/mL) exposure decreased the cells' viability by 44.6% compared to untreated control. Low concentration (2.5nM) of oleic acid slightly suppressed the cell survival by 9.1% analyzed 24hours after incubation. However, oleic acid pretreatment before LPS exposure significantly increased cell survival loss to 63.9%. LPS exposure decreased the expressions of catalase (CAT) and glutathione peroxidase (GPx) mRNA levels by 2.8 and 2.5 fold, respectively. Moreover, pretreatment of the cells with oleic acid strengthened LPS-decreased expressions of CAT and GPx genes by 3.5 and 6.7 fold, respectively. The mRNA expressions of superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), interleukin-1 beta, IL-12, COX-2, caspase-3 and caspase-8 were increased by 2.4, 2.2, 2.2, 2.3, 3.0, 2.6, and 2.5 fold, respectively, by LPS, and oleic acid pretreatment significantly potentiated the effect of LPS. Conclusion: Oleic acid worsens LPS-induced cell death by potentiating oxidative stress and inflammation in A549 lung epithelial cells.en_US
dc.identifier.doi10.1080/01902148.2016.1267823
dc.identifier.endpage7en_US
dc.identifier.issn0190-2148
dc.identifier.issn1521-0499
dc.identifier.issue1en_US
dc.identifier.pmid28080141en_US
dc.identifier.scopus2-s2.0-85009236624en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1080/01902148.2016.1267823
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18695
dc.identifier.volume43en_US
dc.identifier.wosWOS:000399477900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofExperimental Lung Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectInflammationen_US
dc.subjectLipopolysaccharideen_US
dc.subjectLung Injuryen_US
dc.subjectOleic Aciden_US
dc.subjectRespiratory-Distress-Syndromeen_US
dc.subjectAcute Lung Injuryen_US
dc.subjectOxidative Stressen_US
dc.subjectNitric-Oxideen_US
dc.subjectApoptosisen_US
dc.subjectProtectionen_US
dc.titleLow concentration of oleic acid exacerbates LPS-induced cell death and inflammation in human alveolar epithelial cellsen_US
dc.typeArticleen_US

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