Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats

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dc.authoridKARACA, Turan/0000-0002-2500-7781;
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.authorwosidkaraboga, ihsan/AAZ-9840-2020
dc.authorwosiddemirtaş, selim/JED-6784-2023
dc.contributor.authorKaraboga, Ihsan
dc.contributor.authorDemirtas, Selim
dc.contributor.authorKaraca, Turan
dc.date.accessioned2024-06-12T11:03:42Z
dc.date.available2024-06-12T11:03:42Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective(s): This study was aimed at investigating immune activations of the 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model in colonic mucosa by immunohistochemical and Western blot methods. Materials and Methods: For this purpose, 16 female Wistar albino rats were divided into two random groups of control (n=8) and colitis (n=8). The experimental colitis model was induced by intracolonicadministration of TNBS (25 mg/rat). Control animals received only rectal saline for the same time. The animals were sacrificed on the 15th day after TNBS administration, and colon tissue was removed and examined morphologically. Colon samples were stained immunohistochemically with anti-CD3, anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-CD45, anti-TNF-alpha, anti-IL-17, anti-IL-22 and anti-IL-23 antibodies. Additionally, the colonic tissue IL-17 and IL-22 expressions were examined by the Western blot method. Results: In the experimental results, it was determined that there was a significant decrease in body weight and an increase in colon weight in the colitis group when comparing initial experiments. The colon tissue ulcerations, inflammation, crypt loss and Goblet cell loss were observed in the colitis group in microscopic examinations. The immunohistochemical positive cell numbers significantly increased in the colitis group. The immunoreactive lymphocytes in the propria, intracryptal and submucosal layers were found to be increased in the colitis group of rats. In addition, IL-17 and IL-23 expressions were increased in colitis colon mucosa found by Western blot analysis. Conclusion: The Th17/IL-23 pathway and IL-22 serve important roles in the pathogenesis of ulcerative colitis, and will be further examined by study.en_US
dc.description.sponsorshipResearch Fund of the Trakya University [2014/20]en_US
dc.description.sponsorshipThe results presented in this paper were part of Doctoral thesis. The project was supported by Research Fund of the Trakya University. Project number: 2014/20 funding agency.en_US
dc.identifier.doi10.22038/IJBMS.2017.9108
dc.identifier.endpage879en_US
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue8en_US
dc.identifier.pmid29085578en_US
dc.identifier.scopus2-s2.0-85026908536en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage870en_US
dc.identifier.urihttps://doi.org/10.22038/IJBMS.2017.9108
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21760
dc.identifier.volume20en_US
dc.identifier.wosWOS:000409211300005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMashhad Univ Med Sciencesen_US
dc.relation.ispartofIranian Journal Of Basic Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmunohistochemistryen_US
dc.subjectRaten_US
dc.subject2,4,6-Trinitrobenzeneen_US
dc.subjectSulfonic Aciden_US
dc.subjectUlcerative Colitisen_US
dc.subjectWestern Bloten_US
dc.subjectCd8(+) T-Cellsen_US
dc.subjectUlcerative-Colitisen_US
dc.subjectAdhesion Moleculesen_US
dc.subjectTh17 Cellsen_US
dc.subjectTnf-Alphaen_US
dc.subjectExpressionen_US
dc.subjectCytokineen_US
dc.subjectIl-22en_US
dc.subjectInflammationen_US
dc.subjectEffectoren_US
dc.titleInvestigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in ratsen_US
dc.typeArticleen_US

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