Effects of a Mixture of Volatile Organic Compounds on Total DNA and Gene Expression of Heat Shock Proteins in Drosophila melanogaster

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.date.accessioned2024-06-12T11:17:33Z
dc.date.available2024-06-12T11:17:33Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe genotoxic effects of a mixture of 13 volatile organic compounds (VOCs) on total DNA profiles and the expression of heat shock proteins (HSPs) HSP26, HSP60, HSP70, and HSP83 in fruit fly tissues were examined. Drosophila melanogaster Oregon R+, reared under controlled conditions on artificial diets, was treated with 13 VOCs commonly found in water at concentrations of 10, 20, 50, and 75 ppb for 1 and 5 days. Band changes were clearly detected in random amplified polymorphic DNA assay, especially at the 50- and 75-ppb exposure levels, for both treatment periods. In addition, there were clear differences in the band profiles of the treated and untreated flies with changes in the band intensity and the loss/appearance of bands. Although the genomic template stability (GTS) exhibited irregular changes at the first day, significant decreases in GTS were observed after 5 days of VOC application. The lowest GTS value (27.77 +/- A 1.96 %) was detected at the 75-ppb level after 5 days of the treatment. Quantitative real-time polymerase chain reaction analysis showed a significant increase in the relative expression of HSP26 and HSP60 after 1 and 5 days of the treatment, respectively. The expression of HSP70 increased significantly at all treatment concentrations and times. However, the greatest increase in expression level of HSP70 (4.2-fold) occurred at 20 ppb after 5 days of the treatment. HSP83 was the least affected by exposure to the VOCs. We conclude that trace levels of a mixture of VOCs can exert genotoxic effects on both total DNA and HSP levels in Drosophila.en_US
dc.description.sponsorshipT. R. State Planning Organization [2011K120390]en_US
dc.description.sponsorshipThe author is grateful to Dr. Yener Yoruk (Rector of Trakya University, Edirne/Turkey) and the Technology Research and Application Centre, which is funded by T. R. State Planning Organization (Project No. 2011K120390) for providing laboratory equipment.en_US
dc.identifier.doi10.1007/s00244-014-0089-4
dc.identifier.endpage404en_US
dc.identifier.issn0090-4341
dc.identifier.issn1432-0703
dc.identifier.issue2en_US
dc.identifier.pmid25352441en_US
dc.identifier.scopus2-s2.0-84927173394en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage395en_US
dc.identifier.urihttps://doi.org/10.1007/s00244-014-0089-4
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24754
dc.identifier.volume68en_US
dc.identifier.wosWOS:000348206800018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives Of Environmental Contamination And Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmplified Polymorphic Dnaen_US
dc.subjectOxidative Stressen_US
dc.subjectCell-Deathen_US
dc.subjectGenotoxicityen_US
dc.subjectTolueneen_US
dc.subjectHsp70en_US
dc.subjectInductionen_US
dc.subjectExposureen_US
dc.subjectBenzeneen_US
dc.subjectXyleneen_US
dc.titleEffects of a Mixture of Volatile Organic Compounds on Total DNA and Gene Expression of Heat Shock Proteins in Drosophila melanogasteren_US
dc.typeArticleen_US

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