Genotoxic Effect and Carcinogenic Potential of a Mixture of As and Cd in Zebrafish at Permissible Maximum Contamination Levels for Drinking Water

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorid/0000-0003-4135-2486
dc.authoridGokalp, Fulya Dilek/0000-0001-8219-6657
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosid/A-1666-2016
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorMuranli, Fulya Dilek Gokalp
dc.contributor.authorGuner, Utku
dc.date.accessioned2024-06-12T10:52:31Z
dc.date.available2024-06-12T10:52:31Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractCurrently, the toxic effects and carcinogenic potential of individually treated arsenic (As) or cadmium (Cd) are well documented both in animal and human tissues. However, there are no data focusing on the genotoxicity of these heavy metals as a mixture at the very low concentrations of permissible limits for drinking water. In this study, we examine the genotoxicity and carcinogenic potential of single and combined treatments of As and Cd, as well as attempt to elucidate the mechanism of action of certain cell defense systems such as antioxidants, gene repair, heat shock, cell cycle control, and the apoptosis pathway. Zebrafish (Danio rerio), reared under controlled conditions with artificial diets, were treated with As and Cd, either individually or in combination, at concentrations commonly found in water (10 ppb for As and 5 ppb for Cd) and tenfold higher concentrations for 48 h. Our results indicate that separately, As and Cd treatments at low dose selectively induce antioxidant enzymes, gene repair, and caspaseindependent apoptosis in gill tissue, by targeting the mitochondria, leading to oxidative stress and sub-lethal levels of DNA damage. However, tenfold higher (100 ppb As + 50 ppb Cd) treatment caused significant downregulation of genes involved in double-strand break repair and molecular chaperone genes. Additionally, the highest BCL2/BAX ratio (1.6) and lowest expression levels of caspase-3 (8.4-fold) in all treated groups were observed in same condition. These results demonstrate that both single and combined exposure to As and Cd at permissible levels is potentially safe and causes repairable genotoxicity in gill tissue. However, the highest concentration is potentially carcinogenic due to ineffective DNA repair and insufficient apoptosis.en_US
dc.description.sponsorshipT.R. State Planning Organization [2011K120390]en_US
dc.description.sponsorshipThe authors are grateful to Prof. Dr. Yener Yoruk and the Technology Research and Application Centre (TUTAGEM), which is funded by the T.R. State Planning Organization (Project Number 2011K120390), for providing the laboratory equipment.en_US
dc.identifier.doi10.1007/s11270-016-2779-1
dc.identifier.issn0049-6979
dc.identifier.issn1573-2932
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84958741648en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11270-016-2779-1
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18738
dc.identifier.volume227en_US
dc.identifier.wosWOS:000371427300020en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer International Publishing Agen_US
dc.relation.ispartofWater Air And Soil Pollutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArsenicen_US
dc.subjectCadmiumen_US
dc.subjectGenotoxicityen_US
dc.subjectApoptosisen_US
dc.subjectCarcinogenesisen_US
dc.subjectGene Expressionen_US
dc.subjectMismatch-Repairen_US
dc.subjectGene-Expressionen_US
dc.subjectDna-Damageen_US
dc.subjectEscherichia-Colien_US
dc.subjectExcision-Repairen_US
dc.subjectCadmiumen_US
dc.subjectApoptosisen_US
dc.subjectCopperen_US
dc.subjectAmphipoden_US
dc.subjectZincen_US
dc.titleGenotoxic Effect and Carcinogenic Potential of a Mixture of As and Cd in Zebrafish at Permissible Maximum Contamination Levels for Drinking Wateren_US
dc.typeArticleen_US

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