Investigation of Antimicrobial and Antibiofilm Effects of Some Preservatives Used in Drugs, Cosmetics and Food Products

Basit Öğe Kaydı
dc.authorwosidONURDAG, Fatma KAYNAK/T-2518-2017
dc.contributor.authorGuven, Nihal
dc.contributor.authorKaynak Onurdag, Fatma
dc.date.accessioned2024-06-12T11:12:50Z
dc.date.available2024-06-12T11:12:50Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPreservatives are added to food, drugs and other pharmaceutical products to avoid microbial contamination. For antimicrobial activity testing and preservative efficacy testing, vegetative forms of the standard test organisms are used. However, microbial biofilm formation may occur on living tissues, medical implants, industrial or drinking water pipes, natural aquatic systems, glass and plastic surfaces. In our study, it was aimed to determine the antimicrobial and antibiofilm effects of some preservatives used in drug, cosmetics and food products and to compare the minimum biofilm inhibitory concentration (MBIC) of microbial biofilm formed on glass surfaces which are commonly used in those areas and the minimum inhibitory concentration (MIC) values of the planktonic forms. In the study Pseudomonas aeruginosa ATCC 27853, Salmonella Thyphimurium SL1344, Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis NCTC 11047, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 10231 were used as the standard strains; sodium nitrate, methylparaben, prophylparaben, potassium sorbate and sodium benzoate as the preservatives; ampicillin, vancomycin, gentamicin, ciprofloxacin, amphotericin B and itraconazole as the antimicrobial agents. MIC values were determined through the guidelines of CLSI M100-S18 and M27-A3 protocols. BioTimer method was used to determine the MBIC values. The value of colony forming unit (CFU)/glass beads was calculated using the graphics drawn by plotting the time of color change for phenol red or resazurin against log(10)CFU. All experiments were done with four media at different pH values namely pH: 7, pH: 6.5, pH: 6 and pH: 5.5. According to the results of tests on planktonic forms of the microorganisms, sodium benzoate was determined to be the most effective preservative against all the microorganisms tested except S.aureus and E.faecalis. The most effective preservative against S.aureus and E.faecalis was prophylparaben. Prophylparaben was also effective against S.epidermidis. However, in our study it was determined that preservatives were not effective against biofilm forms even if the inoculum was lower, equal to or higher than the inocula of the planktonic forms. The data obtained from this study indicated that preservatives used to prevent microbial contamination in pharmaceutical, cosmetic and food products, are not effective against biofilm forms of the microorganisms. This study is thought to be a guide for further studies to be held in the investigation of antimicrobial and antibiofilm effects of preservatives used in drugs, cosmetics and food industry.en_US
dc.identifier.endpage105en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid24506719en_US
dc.identifier.scopus2-s2.0-84897636250en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage94en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23320
dc.identifier.volume48en_US
dc.identifier.wosWOS:000332131600009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPreservativesen_US
dc.subjectBiofilmen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectIn-Vitro Biofilmen_US
dc.subjectComparative Susceptibilityen_US
dc.subjectPseudomonas-Aeruginosaen_US
dc.subjectConsumer Useen_US
dc.subjectSalmonellaen_US
dc.subjectResistanceen_US
dc.subjectSpp.en_US
dc.titleInvestigation of Antimicrobial and Antibiofilm Effects of Some Preservatives Used in Drugs, Cosmetics and Food Productsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu