Diyabetik sıçanların testis dokularında fukoidan etkilerinin araştırılması
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada; güçlü bir antioksidan olan fukoidanın, diyabet ile birlikte ortaya çıkan hiperglisemi ve oksidatif strese karşı, diyabetik erkek infertilite tedavisinde kullanılabilirliği araştırılmıştır. Yirmi dört adet Wistar albino cinsi erkek sıçan kullanılarak; 1.grup: Kontrol grubu (n=6), 2.grup: Diyabet grubu; 40 mg/kg STZ, 5 ardışık gün ip. (n=6), 3.grup: 40 mg/kg STZ+50 mg/kg ip. fukoidan (Son STZ enjeksiyonundan sonra, 6 hafta boyunca, günaşırı, n=6), 4.grup: 40 mg/kg STZ+50 mg/kg ip. fukoidan (diyabet oluşturulduktan 15 gün sonra, 6 hafta süresince, günaşırı, n=6) olmak üzere gruplar oluşturulmuştur. Kan glukoz düzeyleri; 1. gruba göre, diğer tüm gruplarda anlamlı derecede yüksek bulunmuştur (P<0.05). Seminifer tübül çapları ise 1. grup ile kıyaslandığında, diğer gruplarda anlamlı olarak azalırken (P=0.009, P=0.006, P=0.009); 3. grupta ise 2. gruba göre anlamlı bir artış tespit edilmiştir (P=0.006). Bazal membran kalınlıkları; 2. ve 4. gruplarda, 1. gruba göre anlamlı olarak artarken (P=0.028, P=0.011), 3. ile 1. grup arasında anlamlı bir fark tespit edilmemiştir (P=0.855). Üçüncü ve 4. grupların bazal membran kalınlıkları, 2. gruba göre istatistiksel anlamlılıkla azalmıştır (sırasıyla P=0.015, P= 0.032). Johnsen skoru sonuçları; tüm gruplar, 1. grupla karşılaştırıldığında anlamlı olarak azalırken (P<0.05); 2. gruba göre, 3. ve 4. gruplarda istatistiksel anlamlılıkla artmıştır (P=0.003, P=0.003). Apoptotik tübül ve hücre indeksi; 1. gruba göre 2., 3. ve 4. gruplarda anlamlı derecede artarken (P=0.008, P=0.006, P=0.008), 3. ve 4. grupların değerlerinde 2. gruba göre istatistiksel bir azalma tespit edilmiştir (sırasıyla P=0.035, P=0.009). Sonuç olarak; diyabetin neden olduğu erkek infertilite olgularında, fukoidanın koruyucu etki gösterebileceği kanısındayız.
In this study, fucoidan which is a powerful antioxidant has investigated availability on treatment of diabetic male infertility. Twenty four Wistar albino rats were divided into four groups; 1.group: Control group, 2.group: 40 mg/kg STZ, ip., 5 consecutive days), 3.group: 40 mg/kg STZ+50 mg/kg fucoidan ip. (after the last STZ injection, every other day, during six weeks), 4.group: 40 mg/kg STZ+50 mg/kg fucoidan ip. (after fifteen days induction of diabetes, every other day during six weeks). The seminiferous tubules diameters has been significantly decreased in other groups (P=0.009, P=0.006, P=0.009) compared with the 1st group; in the 3rd groups values has been significantly increased compared with the 2nd group (P=0.006). The thickness of basement membrane has been statistically increased in the 2nd and 4th groups when compared with 1st group (P=0.028, P=0.011), no significant difference has been detected between 3rd and 1st groups (P=0.855). In the 3rd and 4th groups values were significantly decreased compared to 2nd group (P=0.015, P=0.032, respectively). Johnsen score values were significantly decreased in all groups compared with 1st group (P<0.05). There were significantly increased in 3rd and 4th groups compared to 2nd group. Apoptotic tubule and cell indices in the experiment groups were significantly increased compared to 1st group (P=0.008, P=0.006, P=0.008, respectively). It was statistically reduced in the 3rd and 4th groups compared with the 2nd group. As a result of this study, we think fucoidan may show a protective effect in the infertility cases caused by diabetes.
In this study, fucoidan which is a powerful antioxidant has investigated availability on treatment of diabetic male infertility. Twenty four Wistar albino rats were divided into four groups; 1.group: Control group, 2.group: 40 mg/kg STZ, ip., 5 consecutive days), 3.group: 40 mg/kg STZ+50 mg/kg fucoidan ip. (after the last STZ injection, every other day, during six weeks), 4.group: 40 mg/kg STZ+50 mg/kg fucoidan ip. (after fifteen days induction of diabetes, every other day during six weeks). The seminiferous tubules diameters has been significantly decreased in other groups (P=0.009, P=0.006, P=0.009) compared with the 1st group; in the 3rd groups values has been significantly increased compared with the 2nd group (P=0.006). The thickness of basement membrane has been statistically increased in the 2nd and 4th groups when compared with 1st group (P=0.028, P=0.011), no significant difference has been detected between 3rd and 1st groups (P=0.855). In the 3rd and 4th groups values were significantly decreased compared to 2nd group (P=0.015, P=0.032, respectively). Johnsen score values were significantly decreased in all groups compared with 1st group (P<0.05). There were significantly increased in 3rd and 4th groups compared to 2nd group. Apoptotic tubule and cell indices in the experiment groups were significantly increased compared to 1st group (P=0.008, P=0.006, P=0.008, respectively). It was statistically reduced in the 3rd and 4th groups compared with the 2nd group. As a result of this study, we think fucoidan may show a protective effect in the infertility cases caused by diabetes.
Açıklama
Yüksek Lisans
Anahtar Kelimeler
Histoloji ve Embriyoloji, Histology and Embryology
