Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods
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Tarih
2019
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info:eu-repo/semantics/openAccess
Özet
Objectives: Liver transplantation is increasingly being used in the treatment of end-stage liver disease.Ischemia-reperfusion injury is one of the major problems encountered in transplantation. In this study, weaimed to compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemiareperfusion injury with different methods such as biochemical/ultrastructural changes and hepatobiliaryscintigraphy.Methods: Thirty rabbits were used in the Laboratory of Experimental Animals of Trakya University underappropriate conditions. Sham laparotomy and only ischemia reperfusion group were planned. They were usedmelatonin, pentoxifylline, and DMSO after I-R in the other three groups. 6 rabbits were randomly selected foreach group. Rabbits in all groups were subjected to liver scintigraphy. Following scintigraphy, 2 cm2 of livertissue was removed to examining for liver antioxidant enzyme levels (superoxide dismutase [SOD] andglutathione peroxidase [GPx]) and for liver electron microscopy.Results: Pentoxifylline and melatonin protected significantly uptake and excretion functions in liverscintigraphy. When the effects of all three substances were examined by electron microscopy, it was found thatthe three substances protected the liver from the effects of ischemia-reperfusion damage at varying rates. Allthree agents were found to protect SOD and GPx from falling in various amounts.Conclusions: Studies to prevent ischemia-reperfusion injury, which may develop as a result of the Pringlemaneuver applied to liver transplantations as well as to liver resections or liver injuries, still maintain theirpopularity. In our study, the effects of agents were identified in three different ways. Ischemia-reperfusioninjury-reducing effect of pentoxifylline gave parallel results with three methods.
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The European Research Journal
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5
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1
