Effects of Expression of Matrix Metalloproteinases and Discoidin Domain Receptors in Ligamentum Flavum Fibrosis in Patients with Degenerative Lumbar Canal Stenosis

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridOrakdogen, Metin/0000-0002-9395-2902
dc.authoriddelen, ozlem/0000-0001-5652-2658
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.contributor.authorTorun, Yusuf Mansur
dc.contributor.authorDelen, Emre
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorDelen, Ozlem
dc.contributor.authorOrakdogen, Metin
dc.date.accessioned2024-06-12T11:07:39Z
dc.date.available2024-06-12T11:07:39Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractStudy Design: This is a retrospective cohort study. Purpose: This study aimed to clarify the role of crosstalk between discoidin domain receptors (DDRs) and matrix metalloproteinases (MMPs) in the ligamentum flavum (LF) fibrosis obtained from patients with degenerative lumbar canal stenosis (DLCS). Overview of Literature: The DDRs, DDR1 and DDR2, are cell surface receptors and have an essential role in collagen fiber accumulation in several fibrotic diseases. MMPs are one of the critical factors in extracellular matrix remodeling and elastic fiber degradation in LF tissues. However, the crosstalk between DDRs and MMPs and the role of this molecular signal in LF fibrosis remain unclear. Methods: A total of 35 patients were divided into two groups in this study. Spinal surgery was performed in 23 of these patients with the diagnosis of DLCS. Twelve patients with lumbar disk herniation (LDH) were included in the control group. On axial T2-weighted magnetic resonance imaging, LF thickness was measured bilaterally at the level of the facet joint. Histology, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot analyses were performed on LF tissue samples. LF tissues were stained with hematoxylin and eosin. In addition, the grade of fibrosis was histologically assessed using Masson trichrome triple staining. DDR1 and DDR2 Western blot analyses were performed. DDR1, DDR2, MMP2, MMP3, MMP9, and MMP13 expression levels were measured using qRT-PCR analysis. Results: The grade of fibrosis and LF thickness were significantly higher in the DLCS patients than in the LDH patients. DDR1 and DDR2 gene expression and protein levels in LF tissues are significantly greater in DLCS samples than in control samples, according to both qRT-PCR and Western blot analyses. In addition, we detected a significant expression of the MMP3, MMP9, and MMP13, which are known to have important roles in extracellular matrix remodeling in DLCS. Furthermore, we discovered a link between DDR protein levels and LF thickness, fibrosis, and MMP3/MMP9. Conclusions: Our results indicate that DDR1, DDR2, and MMP3 and MMP9 signals can be correlated with each other in LF tissues and be promoted LF fibrosis leading to spinal canal narrowing in patients with DLCS.en_US
dc.description.sponsorshipTrakya University Scientific Research [TUBAP-2020/119]; Turkish Neurosurgical Societyen_US
dc.description.sponsorshipThis study was funded by the Trakya University Scientific Research (TUBAP-2020/119). Preparation for publication of this article is partly supported by Turkish Neurosurgical Society.en_US
dc.identifier.doi10.31616/asj.2021.0380
dc.identifier.endpage202en_US
dc.identifier.issn1976-1902
dc.identifier.issn1976-7846
dc.identifier.issue1en_US
dc.identifier.pmid36163678en_US
dc.identifier.scopus2-s2.0-85150270588en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage194en_US
dc.identifier.urihttps://doi.org/10.31616/asj.2021.0380
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22130
dc.identifier.volume17en_US
dc.identifier.wosWOS:000885318900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Soc Spine Surgeryen_US
dc.relation.ispartofAsian Spine Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLigamentum Flavumen_US
dc.subjectFibrosisen_US
dc.subjectDegenerative Lumbar Canal Stenosisen_US
dc.subjectDiscoidin Domain Receptorsen_US
dc.subjectGrowth-Factoren_US
dc.subjectHypertrophyen_US
dc.subjectActivationen_US
dc.subjectInflammationen_US
dc.subjectInvasionen_US
dc.titleEffects of Expression of Matrix Metalloproteinases and Discoidin Domain Receptors in Ligamentum Flavum Fibrosis in Patients with Degenerative Lumbar Canal Stenosisen_US
dc.typeArticleen_US

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