Anticoagulant and antiprotease effects of a novel heparinlike compound from shrimp (Penaeus brasiliensis) and its neutralization by heparinase I

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dc.contributor.authorDemir, M
dc.contributor.authorIqbal, O
dc.contributor.authorDietrich, CP
dc.contributor.authorHoppensteadt, DA
dc.contributor.authorAhmad, S
dc.contributor.authorDaud, AN
dc.contributor.authorFareed, J
dc.date.accessioned2024-06-12T11:14:09Z
dc.date.available2024-06-12T11:14:09Z
dc.date.issued2001
dc.departmentTrakya Üniversitesien_US
dc.description.abstractHeparin is usually obtained from mammalian organs, such as beef lung, beef mucosa, porcine mucosa, and sheep intestinal mucosa. Because of the increased use of heparin in the production of low-molecular-weight heparin (LMWH), there is a growing shortage of the raw material needed to produce LMWHs. A previous report described the structural features of a novel LMWH from the shrimp (Penaeus brasiliensis). In order to compare anticoagulant and antiprotease effects of this heparin, global anticoagulant tests, such as the prothrombin time, activated partial thromboplastin time, thrombin time, and Heptest(R), were used. Amidolytic anti-Xa and anti-IIa activities were also measured. The relative susceptibility of this heparin to flavobacterial heparinase was also evaluated. The United States Pharmacopeia (USP) potency of shrimp heparin (SH) was found to be 28 U/mg. SH produced a concentration-dependent prolongation of all of the clotting tests and exhibited marked inhibition of FXa and FIIa. Heparinase treatment resulted in a marked decrease of the anticoagulant effects and neutralized the in vitro anti-IIa actions. However, the anti-Xa activities were only partially neutralized. Protamine sulfate was only partially effective in neutralizing the anticoagulant and antithrombin effects of SH. SH also produced marked prolongation of activated clotting time, which was neutralized by heparinase but not by protamine sulfate. These results suggest that SH is a strong anticoagulant with comparable properties to mammalian heparins and can be used in the development of clinically useful antithrombotic-anticoagulant drugs.en_US
dc.identifier.doi10.1177/107602960100700110
dc.identifier.endpage52en_US
dc.identifier.issn1076-0296
dc.identifier.issn1938-2723
dc.identifier.issue1en_US
dc.identifier.pmid11190904en_US
dc.identifier.scopus2-s2.0-0035141280en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage44en_US
dc.identifier.urihttps://doi.org/10.1177/107602960100700110
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23816
dc.identifier.volume7en_US
dc.identifier.wosWOS:000166031700010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Incen_US
dc.relation.ispartofClinical And Applied Thrombosis-Hemostasisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectShrimp Heparinen_US
dc.subjectPinaeus Brasiliensisen_US
dc.subjectHeparinlike Compounden_US
dc.subjectInvertebrate Heparinen_US
dc.titleAnticoagulant and antiprotease effects of a novel heparinlike compound from shrimp (Penaeus brasiliensis) and its neutralization by heparinase Ien_US
dc.typeArticleen_US

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