Melatonin attenuates caspase-dependent apoptosis in the thoracic aorta by regulating element balance and oxidative stress in pinealectomised rats

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dc.authoridDOĞAN, AYTEN/0000-0003-2525-785X
dc.authoridUZUN, Metehan/0000-0003-1406-5473
dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDOĞAN, AYTEN/ABA-6754-2020
dc.authorwosidUZUN, Metehan/JWR-0343-2024
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorUzun, Metehan
dc.contributor.authorOvali, Mehmet Akif
dc.contributor.authorDogan, Ayten
dc.contributor.authorOngoren, Gulin
dc.contributor.authorDoganlar, Oguzhan
dc.date.accessioned2024-06-12T11:11:54Z
dc.date.available2024-06-12T11:11:54Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to explain the possible mechanisms by which melatonin deficiency results in cardiovascular injury and to investigate the effects of melatonin administration on important signalling pathways and element equilibrium in the thoracic aorta (TA). For this purpose, we analysed the cellular and molecular effects of melatonin deficiency or administration on oxidative stress, DNA damage, molecular chaperone response, and apoptosis induction in TA tissues of pinealectomised rats using ELISA, RAPD, qRT-PCR, and Western blot assays. The results showed that melatonin deficiency led to an imbalance in essential element levels, unfolded or misfolded proteins, increased lipid peroxidation, and selectively induced caspasedependent apoptosis in TA tissues without significantly affecting the Bcl-2/BAX ratio (2.28 in pinealectomised rats, 2.73 in pinealectomised rats treated with melatonin). In pinealectomised rats, the genomic template stability (80.22%) was disrupted by the significantly increased oxidative stress, and heat shock protein 70 (20.96-fold), TNF-alpha (1.73-fold), caspase-8 (2.03-fold), and caspase-3 (2.87-fold) were markedly overexpressed compared with the sham group. Melatonin treatment was protective against apoptosis and inhibited oxidative damage. In addition, melatonin increased the survivin level and improved the regulation of element equilibrium in TA tissues. The results of the study indicate that melatonin deficiency induces TNF-alpha-related extrinsic apoptosis signals and that the administration of pharmacological doses of melatonin attenuates cardiovascular toxicity by regulating the increase in the rate of apoptosis caused by melatonin deficiency in TA tissue of Sprague-Dawley rats.en_US
dc.description.sponsorshipTrakya University Scientific Research Fund [TUBAP 2015/45]en_US
dc.description.sponsorshipThis study was supported by the Trakya University Scientific Research Fund (TUBAP 2015/45).en_US
dc.identifier.doi10.1139/apnm-2018-0205
dc.identifier.endpage163en_US
dc.identifier.issn1715-5312
dc.identifier.issn1715-5320
dc.identifier.issue2en_US
dc.identifier.pmid30058356en_US
dc.identifier.scopus2-s2.0-85060651533en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.1139/apnm-2018-0205
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22973
dc.identifier.volume44en_US
dc.identifier.wosWOS:000456999200007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCanadian Science Publishingen_US
dc.relation.ispartofApplied Physiology Nutrition And Metabolismen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOxidative Stressen_US
dc.subjectIntrinsic And Extrinsic Apoptosis Signallingen_US
dc.subjectCaspasesen_US
dc.subjectMelatoninen_US
dc.subjectDna-Damageen_US
dc.subjectInduced Cardiotoxicityen_US
dc.subjectProtective Roleen_US
dc.subjectNeuronal Lossen_US
dc.subjectZincen_US
dc.subjectAntioxidanten_US
dc.subjectCalcificationen_US
dc.subjectDysfunctionen_US
dc.subjectExpressionen_US
dc.subjectReceptoren_US
dc.titleMelatonin attenuates caspase-dependent apoptosis in the thoracic aorta by regulating element balance and oxidative stress in pinealectomised ratsen_US
dc.typeArticleen_US

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