Effect of Topically Applied Azithromycin on Corneal Epithelial and Endothelial Apoptosis in a Rat Model of Corneal Alkali Burn

Basit Öğe Kaydı
dc.authoridErtekin, yusuf haydar/0000-0002-2016-9159
dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.authoridELMAS, Sait/0000-0003-2607-9987
dc.authorwosidErtekin, yusuf haydar/V-7149-2017
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.authorwosiddemirtaş, selim/JED-6784-2023
dc.authorwosidELMAS, Sait/JUU-6671-2023
dc.contributor.authorArikan, Sedat
dc.contributor.authorKaraca, Turan
dc.contributor.authorErtekin, Yusuf Haydar
dc.contributor.authorComez, Arzu Taskiran
dc.contributor.authorErsan, Ismail
dc.contributor.authorDemirtas, Selim
dc.contributor.authorElmas, Sait
dc.date.accessioned2024-06-12T10:52:22Z
dc.date.available2024-06-12T10:52:22Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: To investigate the antiapoptotic effect of topically administered azithromycin (AZM) on corneal epithelial and endothelial cells in a rat model of corneal alkali burn. Methods: Twenty-four Wistar albino rats were divided into 4 equal groups as pseudovehicle (group 1), control (group 2), alkali burned (group 3), and treatment (group 4) groups. Alkali injury was induced only in the right corneas of rats belonging to groups 3 and 4 using 1N NaOH. The rats in group 3 and the rats in group 4 were respectively treated either with an artificial tear gel or with 1.5% AZM eye drops for 5 days. At the fifth day of the experiment, the apoptosis in the corneal epithelium and endothelium of all rats was assessed using a terminal dUTP nick-end labeling (TUNEL) assay. In addition, tumor necrosis factor-alpha (TNF-alpha) density in the corneal epithelium was measured in all rats. Results: The mean numbers of TUNEL+ cells in the corneal epithelium and endothelium of rats in group 3 were 117.1 +/- 23.8 and 34.6.+/- 11.3, respectively, whereas in group 4, they were 75.8 +/- 15.7 and 14.7 +/- 3.5, respectively. Also the mean TNF-alpha densities in the corneal epithelium in group 3 and group 4 were 2.65 +/- 1.3 and 1.65 +/- 1.1, respectively. There was a significant decrease in the mean number of TUNEL+ cells in the corneal epithelium and endothelium and in the mean TNF-alpha density in the corneal epithelium of rats in group 4, when compared with group 3. Conclusions: Topically applied AZM can decrease TNF-alpha-induced apoptosis in corneal alkali burn.en_US
dc.identifier.doi10.1097/ICO.0000000000000730
dc.identifier.endpage549en_US
dc.identifier.issn0277-3740
dc.identifier.issn1536-4798
dc.identifier.issue4en_US
dc.identifier.pmid26751994en_US
dc.identifier.scopus2-s2.0-84954553072en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage543en_US
dc.identifier.urihttps://doi.org/10.1097/ICO.0000000000000730
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18688
dc.identifier.volume35en_US
dc.identifier.wosWOS:000372350000021en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofCorneaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCorneal Alkali Burnen_US
dc.subjectAzithromycinen_US
dc.subjectTnf-Alphaen_US
dc.subjectCytokine Productionen_US
dc.subjectKappa-Ben_US
dc.subjectEfficacyen_US
dc.subjectExpressionen_US
dc.subjectModulationen_US
dc.subjectInfliximaben_US
dc.subjectEyedropsen_US
dc.subjectSafetyen_US
dc.titleEffect of Topically Applied Azithromycin on Corneal Epithelial and Endothelial Apoptosis in a Rat Model of Corneal Alkali Burnen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu