Genotyping of Giardia intestinalis Isolates in the Thrace Region, Turkey

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dc.authoridSAKRU, NERMIN/0000-0002-1312-7233
dc.authorwosidSAKRU, NERMIN/M-9952-2017
dc.contributor.authorCicek, Cemal
dc.contributor.authorSakru, Nermin
dc.date.accessioned2024-06-12T11:02:37Z
dc.date.available2024-06-12T11:02:37Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractGiardia intestinalis is a common protozoon that infects humans and may cause water and food-borne outbreaks. It is regarded as a major public health problem worldwide and in Turkey as well. Molecular techniques are widely used to determine the epidemiology, genetic populations and taxonomy of G.intestinalis. It has two genotypes including genotype A and genotype B in humans. The purpose of the present study is to implement the molecular analysis and genotyping of the isolates of G.intestinalis obtained from human stool samples. A total of 39 isolates obtained from the stool samples of persons (30 male, 9 female; age range: 1-74 years, median age: 20) who have admitted to Trakya University Medical Research and Practice Health Center and Edirne State Hospital between September 2011- April 2013 were included in the study. The average number of cysts were identified both with native and lugol methods among all microscopically detected samples by screening at least 50 field with x400 magnification. The samples were then analyzed through loop-mediated isothermal amplification method (LAMP) for the presence of elongation factor-1 alpha (EF-1 alpha) gene, and polymerase chain reaction (PCR) method for the presence of beta-giardin (bg) gene regions. In addition, sequence analysis of bg gene was performed. Of 39 samples, 32 (82%) and 19 (48.7%) were found to be positive for G.intestinalis EF-1 alpha and bg genes by LAMP and PCR methods, respectively. Genotyping was implemented in 17 out of 19 samples yielding nine genotype A and eight genotype B strains. The sub-genotypes of these strains were identified as A2 (n=6), A3 (n=3), B2 (n=6), B3 (n=1) and B4 (n=1). In eight isolates that could be typed among 21 symptomatic patients, genotype B (n=5) and in nine isolates that could be typed among 18 asymptomatic patients, genotype A (n=6) were more frequently observed. There was no significant association between symptomatic or asymptomatic status and genotypic patterns of the cases (p=0.347). The PCR positivity rate showed a significant difference between patients with higher cyst density and lower cyst density (p=0.0001). In conclusion, molecular methods such as LAMP and PCR might have the potential to provide a substantial guidance for the analysis of outbreaks. In addition, the determined subtypes of G.intestinalis in our region is expected to contribute to the global epidemiological data.en_US
dc.identifier.endpage585en_US
dc.identifier.issn0374-9096
dc.identifier.issue4en_US
dc.identifier.pmid26649415en_US
dc.identifier.startpage576en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21361
dc.identifier.volume49en_US
dc.identifier.wosWOS:000366227900011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGiardia Intestinalisen_US
dc.subjectLAMPen_US
dc.subjectGenotypeen_US
dc.subjectEpidemiologyen_US
dc.subjectTurkeyen_US
dc.subjectFragment-Length-Polymorphismen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectDuodenalisen_US
dc.subjectAssemblageen_US
dc.subjectLambliaen_US
dc.subjectChildrenen_US
dc.subjectSamplesen_US
dc.subjectGeneen_US
dc.titleGenotyping of Giardia intestinalis Isolates in the Thrace Region, Turkeyen_US
dc.typeArticleen_US

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