Antiapoptotic effect of l-carnitine on testicular irradiation in rats

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dc.authoridPARLAR, SULE/0000-0001-9422-853X
dc.contributor.authorKanter, Mehmet
dc.contributor.authorTopcu-Tarladacalisir, Yeter
dc.contributor.authorParlar, Sule
dc.date.accessioned2024-06-12T11:04:02Z
dc.date.available2024-06-12T11:04:02Z
dc.date.issued2010
dc.departmentTrakya Üniversitesien_US
dc.description.abstractWe evaluated the effects of l-carnitine on apoptosis of germ cells in the rat testis following irradiation. Male Wistar rats were divided into three groups. Control group received sham irradiation plus physiological saline. Radiotherapy group received scrotal gamma-irradiation of 10 Gy as a single dose plus physiological saline. Radiotherapy + l-carnitine group received scrotal irradiation plus 200 mg/kg intraperitoneally l-carnitine. Twenty-four hours post-irradiation, the rats were sacrificed and testes were harvested. Testicular damage was examined by light and electron microscopy, and germ cell apoptosis was determined by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) technique. Morphologically, examination of irradiated testis revealed presence of disorganization and desquamation of germinal cells and the reduction in sperm count in seminiferous tubule lumen. Under electron microscopy, the morphological signs of apoptosis were frequently detected in spermatogonia. Apoptotic spermatogonia showed the marginal condensation of chromatin onto the nuclear lamina, nucleus and cytoplasm shrinkage and still functioning cell organelles. TUNEL-positive cells were significantly more numerous in irradiated rats than in control rats. l-carnitine treatment significantly attenuated the radiation-induced morphological changes and germ cell apoptosis in the irradiated rat testis. In conclusion, these results suggested that l-carnitine supplementation during the radiotherapy may be beneficial for spermatogenesis following testicular irradiation by decreasing germ cell apoptosis.en_US
dc.identifier.doi10.1007/s10735-010-9267-5
dc.identifier.endpage128en_US
dc.identifier.issn1567-2379
dc.identifier.issn1567-2387
dc.identifier.issue2-3en_US
dc.identifier.pmid20446105en_US
dc.identifier.scopus2-s2.0-77955927534en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage121en_US
dc.identifier.urihttps://doi.org/10.1007/s10735-010-9267-5
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21886
dc.identifier.volume41en_US
dc.identifier.wosWOS:000280918200004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Molecular Histologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIrradiationen_US
dc.subjectL-Carnitineen_US
dc.subjectTesticular Toxicityen_US
dc.subjectGerm Cell Apoptosisen_US
dc.subjectRaten_US
dc.subjectGerm-Cell Apoptosisen_US
dc.subjectInduced Oxidative Stressen_US
dc.subjectL-Acetylcarnitine Lacen_US
dc.subjectSingle-Strand Breaksen_US
dc.subjectAcetyl-L-Carnitineen_US
dc.subjectDifferential Expressionen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectFlow-Cytometryen_US
dc.subjectMouse Testisen_US
dc.subjectVitamin-Een_US
dc.titleAntiapoptotic effect of l-carnitine on testicular irradiation in ratsen_US
dc.typeArticleen_US

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