Maleik hidrazid'in insan lenfositlerinde sitogenetik etkisi
Küçük Resim Yok
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
ÖZET Bu çalışmada Maleik Hidrazid'in farklı konsantrasyon ve muamele sürelerinde insan lenfositleri üzerindeki sitogenetik etkisi periferal kan kültürü yöntemiyle araştırılmıştır. Bu amaçla distile suda eritilen Maleik Hidrazid'in 75 ug/ml, 300 ug/ml, 500 ug/ml ve 1000 ug/ml'lik konsantrasyonları kullanılmıştır. İnsan lenfositleri farklı konsantrasyonlarda^ Maleik Hidrazid ile 24 ve 48 saat süre boyunca muamele edilmiştir. Maleik Hidrazid insan lenfositlerinde kromatid gap, kromatid kırık, izokromatid gap, izokromatid kırık, spiral çözülmesi ve kontraksiyon gibi kromozom aberasyonlarına neden olmuştur. Yapılan istatistikler ( Fisher's Exact X2 ), meydana gelen aberasyonların Maleik Hidrazid'in 75 ug/ml'lik konsantrasyonu ile 24 saat muamele edilmiş grup dışındaki tüm gruplarda kontrole göre anlamlı olduğunu göstermiştir. Maleik Hidrazid, artan konsantrasyon ve muamele süresine bağlı olarak mitotik indeksi de azaltmıştır. Araştırmamızda elde edilen sitogenetik ve istatistiksel sonuçlara göre Maleik Hidrazid insan lenfositleri üzerinde genotoksik etkilidir. Anahtar Kelimeler Maleik Hidrazid, kromozom aberasyonları, periferal kan insan lenfosit kültürü.
II SUMMARY In this study, the cytogenetic effects of Maleic Hydrazide at different concentrations and application periods were investigated by the method of peripheral blood culture. For this purpose, 75 ug/ml, 300 ug/ml, 500 ug/ml and 1000 ug/ml concentration of Maleic Hydrazide which was melted in distilled water were used. Human lymphocytes were applied with Maleic Hydrazide at different concentrations for 24 and 48 hours. Maleic Hydrazide induced chromosome aberrations like chromatid gap, chromatid break, isochromatid gap, isochromatid break, chromosome despiralisation and contraction on human lymphocytes. Statistical tests ( Fisher's Exact X2 ) revealed that, according to the control group, ocurrent aberrations at all the application groups except the group which was applied 75 ug/ml concentration of Maleic Hydrazide for 24 hours, were significant. Maleic Hydrazide also decreased mitotic index depending upon the increasing concentrations and application periods. According to the cytogenetic and statistical results which were obtained in our study, Maleic Hydrazide is genotoxically effective on human lymphocytes. Key Words: Maleic Hydrazide, chromosome aberrations, peripheral blood human lymphocyte culture.
II SUMMARY In this study, the cytogenetic effects of Maleic Hydrazide at different concentrations and application periods were investigated by the method of peripheral blood culture. For this purpose, 75 ug/ml, 300 ug/ml, 500 ug/ml and 1000 ug/ml concentration of Maleic Hydrazide which was melted in distilled water were used. Human lymphocytes were applied with Maleic Hydrazide at different concentrations for 24 and 48 hours. Maleic Hydrazide induced chromosome aberrations like chromatid gap, chromatid break, isochromatid gap, isochromatid break, chromosome despiralisation and contraction on human lymphocytes. Statistical tests ( Fisher's Exact X2 ) revealed that, according to the control group, ocurrent aberrations at all the application groups except the group which was applied 75 ug/ml concentration of Maleic Hydrazide for 24 hours, were significant. Maleic Hydrazide also decreased mitotic index depending upon the increasing concentrations and application periods. According to the cytogenetic and statistical results which were obtained in our study, Maleic Hydrazide is genotoxically effective on human lymphocytes. Key Words: Maleic Hydrazide, chromosome aberrations, peripheral blood human lymphocyte culture.
Açıklama
Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.
Yüksek Lisans
Yüksek Lisans
Anahtar Kelimeler
Biyoloji, Biology
