Determination of plasma microRNA for early detection of gastric cancer

Basit Öğe Kaydı
dc.authoridBALCI, Senay/0000-0002-7498-604X
dc.authoridDoğruer Ünal, Zeynep Nil/0000-0002-4083-0944
dc.authoridAYAZ, LOKMAN/0000-0002-2876-055X
dc.authorwosidserin, mehmet Sami/KCK-8718-2024
dc.authorwosidYildirim, Hatice/O-7121-2016
dc.authorwosidBALCI, Senay/W-4931-2019
dc.authorwosidTamer, Lulufer/AAG-5796-2021
dc.authorwosidDoğruer Ünal, Zeynep Nil/G-2218-2015
dc.authorwosidSerin, Mehmet Sami/G-1572-2015
dc.authorwosidAYAZ, LOKMAN/K-6716-2013
dc.contributor.authorGorur, Aysegul
dc.contributor.authorFidanci, Senay Balci
dc.contributor.authorUnal, Nil Dogruer
dc.contributor.authorAyaz, Lokman
dc.contributor.authorAkbayir, Serin
dc.contributor.authorYaroglu, Hatice Yildirim
dc.contributor.authorDirlik, Musa
dc.date.accessioned2024-06-12T11:02:30Z
dc.date.available2024-06-12T11:02:30Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractGastric cancer is the fourth most prevalent malignancy worldwide and remains the second most common cause of cancer-related death globally. Understanding the molecular structure of gastric carcinogenesis might identify new diagnostic and therapeutic strategies for this disease. Thus, early detection of gastric cancer is a key measure to reduce the mortality and improve the prognosis of gastric cancer. There have recently been several reports that microRNAs (miRNAs) circulate in highly stable, cell-free forms in blood. Because serum and plasma miRNAs are relatively easy to access, circulating miRNAs also have great potential to serve as non-invasive biomarkers. Although a number of miRNAs associated with gastric cancer have been identified, the underlying mechanism of these miRNAs in tumorigenesis and tumor progression remains to be investigated. The purpose of this study is to identify the potential of serum miRNAs as biomarkers for early detection of gastric cancer patients. RNA was isolated using the High Pure miRNA Isolation Kit (Roche) following the manufacturer's protocol. cDNA and preamplification protocols were obtained from the isolated plasma miRNAs. The BioMark (TM) 96.96 Dynamic Array (Fluidigm Corporation) for real-time qPCR was used to simultaneously quantite the expression of 740 miRNAs. All statistical analyses were performed using the Biogazelle's qbase PLUS 2.0 software. In this study, among 740 miRNAs that we analyzed only miR-195-5p was significantly (p < 0.05, fold changes = 13, 3) down-regulated in gastric cancer patients compared with control. We demonstrated that miR-195-5p is a novel tumor suppressor miRNA and may contribute to gastric carcinogenesis. The miRNA expression profile described in this study should contribute to future studies on the role of miRNAs in gastric cancer.en_US
dc.description.sponsorshipCukurova Development Agency (CKA), Mersin, Turkey [TR62-09-03]en_US
dc.description.sponsorshipThis work which is the part of Establishment of Molecular Substructure Laboratory for Early Diagnosis of Cancer (TR62-09-03) project was supported by Grants from Cukurova Development Agency (CKA), Mersin, Turkey.en_US
dc.identifier.doi10.1007/s11033-012-2267-7
dc.identifier.endpage2096en_US
dc.identifier.issn0301-4851
dc.identifier.issue3en_US
dc.identifier.pmid23212612en_US
dc.identifier.scopus2-s2.0-84878392341en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2091en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-012-2267-7
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21293
dc.identifier.volume40en_US
dc.identifier.wosWOS:000314535600005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGastric Canceren_US
dc.subjectMirnasen_US
dc.subjectEarly Detectionen_US
dc.subjectTumor Biomarkeren_US
dc.subjectDiagnosisen_US
dc.subjectCirculating Micrornasen_US
dc.subjectExpressionen_US
dc.subjectCarcinomaen_US
dc.subjectSerumen_US
dc.titleDetermination of plasma microRNA for early detection of gastric canceren_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu