The Role of JAK-STAT Signaling Activation in Hypertrophied Ligamentum Flavum

Basit Öğe Kaydı
dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authoriddelen, ozlem/0000-0001-5652-2658
dc.authorwosidKILINÇER, Cumhur/C-7969-2014
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.contributor.authorDelen, Emre
dc.contributor.authorDaganlar, Oguzhan
dc.contributor.authorDelen, Ozlem
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorKilincer, Cumhur
dc.date.accessioned2024-06-12T10:55:32Z
dc.date.available2024-06-12T10:55:32Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBACKGROUND: Although previous studies have reported the expression of JAK1, STAT3, and phosphorylated STAT3 in hypertrophied ligamentum flavum (LF), the role of the Janus kinaseesignal transducer and activator of transcription (JAK/STAT) signaling pathway in hypertrophied LF has not been fully elucidated. The aim of this study was to identify the important JAK/STAT gene expression patterns of the 3 main receptors involved in this pathway: interferon (IFN)-gamma receptor (IFN-gamma R), IFN-alpha receptor (IFNAR), and interleukin (IL)-6 receptor (IL-6R). METHODS: The human LF specimens were obtained from 28 patients who underwent lumbar spine surgery for either degenerative lumbar canal stenosis (DLCS) (n [ 28) or lumbar disc herniation (LDH) (n [ 20). In this design, patients with LDH served as the control group. The degree of fibrosis was demonstrated by Masson's trichrome staining. The location and expression profiling of the JAK/ STAT pathway were analyzed by quantitative real-time polymerase chain reaction and Western blotting. The thickness of the LF was measured with axial T1-weighted magnetic resonance imaging. RESULTS: The most severe fibrotic changes were on the dorsal side of the LF. IL-6 and IFN-I expression levels were significantly increased on the dorsal side of the LF. While expression levels of IL-6R and IFNAR on the dural and dorsal side were significantly higher in the DLCS samples, IFN-gamma R and endothelial epidermal growth factor receptor in LF samples showed a significant increase only on the dorsal side. JAK/STAT genes were significantly expressed, especially on the dorsal side. CONCLUSIONS: Our data suggest that IFNAR- and IL-6R-dependent JAK/STAT signaling pathways may be significant targets in drug development strategies for the treatment of LF hypertrophy.en_US
dc.identifier.doi10.1016/j.wneu.2020.02.024
dc.identifier.endpageE516en_US
dc.identifier.issn1878-8750
dc.identifier.issn1878-8769
dc.identifier.pmid32059970en_US
dc.identifier.scopus2-s2.0-85080993442en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpageE506en_US
dc.identifier.urihttps://doi.org/10.1016/j.wneu.2020.02.024
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19461
dc.identifier.volume137en_US
dc.identifier.wosWOS:000532720800021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofWorld Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCanal Stenosisen_US
dc.subjectJAK/STAT Signalingen_US
dc.subjectLigamentum Flavumen_US
dc.subjectLumbar Spineen_US
dc.subjectGrowth-Factoren_US
dc.subjectInflammationen_US
dc.subjectExpressionen_US
dc.subjectPathwayen_US
dc.subjectPathomechanismen_US
dc.subjectCytokinesen_US
dc.subjectFibrosisen_US
dc.subjectStenosisen_US
dc.titleThe Role of JAK-STAT Signaling Activation in Hypertrophied Ligamentum Flavumen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu