Cytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Match

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dc.authoridATA, PINAR/0000-0002-6688-2347
dc.authoridBILGEN, TURKER/0000-0002-3015-0929
dc.authorwosidATA, PINAR/AAC-9967-2020
dc.authorwosidBILGEN, TURKER/N-6376-2018
dc.contributor.authorBilgen, T.
dc.contributor.authorAta, P.
dc.contributor.authorTozkir, J.
dc.contributor.authorTozkir, H.
dc.contributor.authorTitiz, M. I.
dc.date.accessioned2024-06-12T11:19:47Z
dc.date.available2024-06-12T11:19:47Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground. Complement-dependent lymphocytotoxicity (CDC-XM) and flowcytometric (FCXM) cross-match are analyzed individually for each donor and recipient pair, because these techniques have fundamental differences for the evaluation of histocompatibility. Lately, cytotoxic flow-cytometric cross-match (cFCXM) has been developed as an alternative to both CDC-XM and FCXM techniques. We evaluated the limits of cFCXM with the use of different positive serum dilutions. Methods. CDC-XM, FCXM, and cFCXM tests were performed with the use of commercially available negative and positive serum samples and lymphocytes from healthy donors. Results. Complement-dependent cell death was successfully detected with the use of cFCXM. Complement-dependent cell death ratios in cFCXM were similar those in CDCXM. With cFCXM, not only complement-dependent cell death but also IgG binding could be detected within a single assay. At higher concentrations of the positive serum, IgG-fluorescein isothiocyanate (FITC) mean fluorescent intensity (MFI) values detected with the use of cFCXM were less than those of conventional FCXM. Correspondingly, for dead cells, MFI values of IgG-FITC were less than those of live cells in higher positive serum concentrations in the cFCXM assay. Moreover, our results demonstrated that in cFCXM analysis, the decreasing ratio of dead cells at increasing positive serum dilutions was not in parallel with the same decrease in IgG-FITC MFI values. Conclusions. The cFCXM technique detects complement-mediated cytotoxic cell death with the additional ability to show IgG binding in the same tube and therefore may reduce the necessary bench time and workload.en_US
dc.description.sponsorshipResearch Foundation Management Unit of Namik Kemal University [NKUBAP.02.GA.16.032]en_US
dc.description.sponsorshipResearch Foundation Management Unit of Namik Kemal University (NKUBAP.02.GA.16.032).en_US
dc.identifier.doi10.1016/j.transproceed.2017.01.016
dc.identifier.endpage444en_US
dc.identifier.issn0041-1345
dc.identifier.issn1873-2623
dc.identifier.issue3en_US
dc.identifier.pmid28340808en_US
dc.identifier.scopus2-s2.0-85015834796en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage440en_US
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2017.01.016
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25345
dc.identifier.volume49en_US
dc.identifier.wosWOS:000398017900012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTransplantation Proceedingsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRenal-Transplantationen_US
dc.subjectBindingen_US
dc.subjectAssayen_US
dc.subjectIggen_US
dc.titleCytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Matchen_US
dc.typeArticleen_US

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