Investigation of inhibition kinetics of various plant extracts on polyphenol oxidase enzyme from Paulownia Tomentosa and binding mechanism by molecular docking

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dc.authoridYilmaz Ozguven, Serap/0000-0002-6458-2658
dc.authoridArabaci, Gulnur/0000-0002-1190-5695
dc.authorwosidYilmaz Ozguven, Serap/G-1553-2014
dc.contributor.authorCesko, Cengiz
dc.contributor.authorArabaci, Gulnur
dc.contributor.authorPaluzar, Hatice
dc.contributor.authorOzguven, Serap Yilmaz
dc.date.accessioned2024-06-12T11:16:00Z
dc.date.available2024-06-12T11:16:00Z
dc.date.issued2024
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPolyphenol oxidase/tyrosinase enzyme, which contains binuclear copper clusters play an important role in melanogenesis and enzymatic browning. Therefore, its inhibitors can be attractive in cosmetics and medicinal industries as depigmentation agents and also in food and agriculture industries as antibrowning compounds. Our aim in this study is to find natural plant extracts that will inhibit the polyphenol oxidase enzyme. The inhibitory effects of Verbascum thapsus, Tanacetum vulgare, Solanum nigrum and Datura stramonium herbal plant extracts obtained from Kosovo on the polyphenol oxidase enzyme activity were investigated. Polyphenol oxidase from the Poulownia tomentosa plant was purified using the three-phase purification method. It is determined that T. vulgare extract showed the most effective inhibitory effect with 0.43 mg/mL IC50 value. In order to explain and support the binding mechanism of the best inhibitor extract, the phenolic content of the T. vulgare extract was determined by LC-MS/MS and the mechanism of the inhibitory effect was explained by performing a molecular docking study for the phenolic compounds it contained at the highest rate.en_US
dc.identifier.doi10.1016/j.mcat.2024.113952
dc.identifier.issn2468-8231
dc.identifier.scopus2-s2.0-85188613759en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.mcat.2024.113952
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24161
dc.identifier.volume557en_US
dc.identifier.wosWOS:001202785700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMolecular Catalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTanacetum Vulgareen_US
dc.subjectPaulownia Tomentosaen_US
dc.subjectPolyphenol Oxidaseen_US
dc.subjectInhibitionen_US
dc.subjectMolecular Dockingen_US
dc.subjectTyrosinaseen_US
dc.subjectConstituentsen_US
dc.subjectChemistryen_US
dc.subjectAciden_US
dc.titleInvestigation of inhibition kinetics of various plant extracts on polyphenol oxidase enzyme from Paulownia Tomentosa and binding mechanism by molecular dockingen_US
dc.typeArticleen_US

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