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Öğe Anatomical and Palynological Studies on Chaerophyllum astrantiae and C-aureum in Turkey(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2013) Yilmaz, Gulden; Tekin, MehmetIn this study, anatomical and palynological properties of Chaerophyllum astrantiae Boiss. & Bal. and C. aureum L. were determined for the first time. Cross sections of roots, stems, stem leaves and fruits of both species were examined. The leaves are amphistomatic and mesophyll is bifacial The fruit has two vittae on commissural face and four vittae on lateral and dorsal faces in both species. The palynological studies revealed that, C. astrantiae and C. aureum have tricolporate pollen type and perforate exine ornamentation under light microscope. Ratio of polar axis to equatorial axis is 2.15 in C. astrantiae and 2.05 in C. aureum. Thus, pollen shape is perprolate for both of the species.Öğe Anatomical investigations of the Turkish critically endangered species: Achillea sivasica Celik et Akpulat (Asteraceae)(Univ Zagreb, Fac Science, Div Biology, 2021) Tekin, Mehmet; Akdere, SeydaIn this study, the root, stem, leaf midrib and leaf lamina anatomy and achene micromorphology of the Turkish critically endangered endemic Achillea sivasica were investigated for the first time. In this study, the root was found in late primary growth and in early secondary growth stage. It has a large cortex layer consisting of 12-16 cell rows beneath the periderm. Secretory ducts formed by 5-12 secretory cells embedded in the cortex and located near the vascular bundle were found at the root, which was in the early stage of secondary development. The stem was circular-pentagonal in cross-section. There was lamellar collenchyma beneath epidermis of pentagon corners, and cortex parenchyma between corners. Secretory ducts located near the phloem, between the cortex and endodermis on the interfascicular region, were also observed. An endodermis layer was evident and its cells have indentations and protrusions where they touch adjacent endodermis cells, which strengthens the connection between them. In addition, casparian strips were conspicuous in many endodermis cells. The leaf midrib area had a triangular cross section. There were secretory ducts, consisting of 4-5 secretory cells observed on both sides of the sclerenchymatous fibers that accompany the xylem. The leaf lamina was amphistomatic and stomata type was anomocytic. Mesophyll layer was equifacial. There was a large secretory duct and its diameter is bigger than the nearest main lamina vascular bundle. Achene shape of A. sivasica was lanceolate-oblong and its surface was ribbed and glabrous.Öğe Anti-inflammatory and antibacterial evaluation of Thymus sipyleus Boiss. subsp sipyleus var. sipyleus essential oil against rhinosinusitis pathogens(Academic Press Ltd- Elsevier Science Ltd, 2018) Demirci, Fatih; Karaca, Nursenem; Tekin, Mehmet; Demirci, BetulThymus sipyleus Boiss. subsp. sipyleus var. sipyleus of the Lamiaceae, locally known as thyme scented lemon, which is an endemic taxon collected from Sivas in Anatolia, was investigated in this study due to its folk medicine use against rhinosinusitis. The aromatic characteristics of the plant material gave the idea for the detailed evaluation of the volatiles and essential oil thereof. Consequently, the oil was obtained by Clevenger type hydrodistillation followed by gas chromatography-flame ionization detector (GC-FID) and gas chromato-graphy/mass spectrometry (GC/MS) analyses for phytochemical characterization. To confirm the folk medicinal use against sinusitis, in vitro antimicrobial activities of the essential oil was evaluated by agar diffusion, microdilution and vapour diffusion methods against selected rhinosinusitis associated strains such as Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. epidermidis, Streptococcus pyogenes, S. pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae and Moraxella catarrhal's. Additionally, the in vitro anti-inflammatory activity was evaluated by 5-lipoxygenase (5-LOX) inhibitory effect of the essential oil spectrophotometrically. Furthermore, the composition of the volatiles of the vapour phase of the oil was determined by headspace-solid phase microextraction (HS-SPME-GC/MS) after 15 min and 24 h in accordance with antimicrobial vapour diffusion method conditions, respectively. According to the analytical results, the main component was determined as thymol (66.2%). Whereas in the HS-SPME method p-cymene (26.1%) and gamma-terpinene (26%) were identified as the main volatile components within the 15. min., and thymol (75.3%) after 24 h, respectively. The antibacterial activity against rhinosinusitis pathogens varied between 160 and 1250 mu g/mL minimum inhibitory concentrations, with the best inhibitory effects observed against the S. aureus, S. pyogenes and M. catarrhalis. The anti-inflammatory activity of the oil was determined as 12.1 +/- 1.8% in 100 mu g/mL. The results showed the in vitro antimicrobial and anti-inflammatory potential of the oil also in vapour phase against sinusitis supporting the traditional use.Öğe Antidiabetic activity evaluation of Onobrychis species on alloxan-induced diabetic mice(Univ Sao Paulo, Conjunto Quimicas, 2019) Ozbek, Hanefi; Acikara, Ozlem Bahadir; Oz, Burcin Ergene; Ozbilgin, Serkan; Kirmizir, Neriman Ipek; Ozrenk, Bade Cevriye; Tekin, MehmetIn the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. subsp. argyrea Boiss., O. galegifolia Boiss., and O. tournefortii (Willd.) Desv. were collected from Anatolia to be evaluated for their antidiabetic activities. Methanol water extracts of the aerial parts were used for experiments. An alloxan-induced diabetic mice test model was used. Phytochemical analysis of the tested extracts was investigated using the HPLC method. The highest activity was observed with treatment of O. albiflora aerial part extract. Significant decrements were detected in the blood glucose levels as follows: 180.83 +/- 47.48 and 252.83 +/- 50.47mg/dL at 100 mg/kg and 200 mg/kg doses of O. albiflora, respectively, when compared to the isotonic saline solution control group, eliciting a blood glucose level of 494.20 +/- 27.32. Among the tested standard compounds, rutin and isoquercetin were detected in the examined species. The highest amount of rutin (1.1981 +/- 0.0017%) and isoquercetin (0.7318 +/- 0.0197%) were found in O. albiflora and O. argyrea subsp. argyrea, respectively. Antidiabetic activities of the tested Onobrychis species seem to indicate a possible correlation with their rutin and isoquercetin contents. Therefore, rutin and isoquercetin may be the antidiabetic compounds that contribute to the antidiabetic activity of the tested Onobrychis species.Öğe Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities(Walter De Gruyter Gmbh, 2021) Goger, Gamze; Allak, Muhammed; Sen, Ali; Goger, Fatih; Tekin, Mehmet; Ozek, GulmiraPhytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and beta-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and alpha-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 mu g/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 mu g/mL, whereas, CAD showed the best inhibition of a-glucosidase with an IC50 value of 396.40 mu g/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 mu g/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.Öğe Bioactivities of Alchemilla mollis, Alchemilla persica and Their Active Constituents(Univ Sao Paulo, Conjunto Quimicas, 2022) Kurtul, Ekin; Eryilmaz, Mujde; Sarialtin, Sezen Yilmaz; Tekin, Mehmet; Acikara, Ozlem Bahadir; Coban, TulayThe aim of this study was to determine antimicrobial activities of Alchemilla mollis, Alchemilla persica as well as ellagic acid and miquelianin against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans by using microbroth dilution method and anti-inflammatory activity by using human red blood cell (HRBC) membrane stabilization method. Microbroth dilution method was used to determine the antimicrobial activities. Extracts possessed activity having MIC values of 2.5-5-10mg/mL, compounds possessed activity having MIC values of 1.25-2.5- 4-5mg/mL. A.mollis aerial parts displayed the highest anti-inflammatory activity (IC50=1.22 +/- 0.07mg/mL). Ellagic acid and miquelianin were also determined as anti-inflammatory agents with 0.57 +/- 0.01mg/mL and 1.23 +/- 0.02mg/mL IC50 values, respectively. Total phenolic content and tannin content of the A.mollis and A.persica were determined as 357.00 +/- 75.80mg, 282.50 +/- 28.70mg PGE/g plant material and 18.02%, 18.63% respectively according to the method described by European Pharmacopoeia. Ellagic acid, miquelianin and catechin were analyzed by HPLC. The highest catechin content was detected in A.persica roots (6.69 +/- 0.05g/100g plant material). A.mollis aerial parts contain higher miquelianin (0.39 +/- 0.02g/plant material) and ellagic acid (1.56 +/- 0.01g/plant material) than A.persica.Öğe BIOLOGICAL ACTIVITY EVALUATION OF THREE Hypericum L. SPECIES(Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2022) Eruygur, Nuraniye; Ozpinar, Necati; Ozpinar, Hulya; Atas, Mehmet; Tekin, Mehmet; Cevik, OzgeThis study was performed in order to evaluate in vitro antioxidant, anti-Trichomonas vaginalis, antimicrobial and cytotoxic activities of methanol extracts of aerial parts of Hypericum perforatum L., H. thymbrifolium Boiss. & Noe and H. thymopsis Boiss. The antioxidant activity of methanol extracts was tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and iron chelating assays. The total phenol and flavonoid contents were also determined spectrophotometrically. Cytotoxicity and apoptosis were evaluated on MCF-7 breast cancer and PC3 prostate cancer cell line. Antimicrobial activities were evaluated by the microdilution method on the bacterial strains Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853) Escherichia coli (ATCC 25922) and the fungal strain Candida albicans (ATCC 10231). All extracts demonstrated cytotoxic activity on PC3 prostat cancer cell line with a concentration dependent manner, while H. thymopsis extract was the most active against cancer cell growth. The obtained results of the present study revealed that the methanol extract of H. perforatum, H. thymbrifolium and H. thymopsis have significant antioxidant and cytotoxic activities. The results provided the basic research data for further phytochemical and biological activity guided investigations on these species for identication and isolation of potential drug active compounds.Öğe Chemical Compositions of Achillea sivasica: Different Plant Part Volatiles, Enantiomers and Fatty Acids(Acg Publications, 2018) Ozek, Gulmira; Tekin, Mehmet; Haliloglu, Yesim; Baser, Kemal Husnu Can; Ozek, Temel[Abstract Not Available]Öğe Chemical Compositions of Achillea sivasica: Different Plant Part Volatiles, Enantiomers and Fatty Acids(2018) Özek, Gülmira; Tekin, Mehmet; Haliloğlu, Yeşim; Başer, Kemal Hüsnü Can; Özek, TemelIn the present work, Microsteam distillation - Solid phase microextraction (MSD-SPME) andhydrodistillation (HD) techniques were applied to obtain volatiles from Achillea sivasica, an endemic species fromTurkey. GC-FID and GC/MS analysis revealed that 1,8-cineole (22.1%) and ?-pinene (9.3%) were the mainconstituents of the hydrodistilled flower volatiles. (Z)-?-Farnesene (23.9%), decanoic acid (10.1%), ??eudesmol(8.0%), tricosane (7.3%) and hexadecanoic acid (7.2%) were the main volatiles obtained from flowers by MSDSPME.The leaf volatiles obtained by HD contained camphor (9.0%), ?-pinene (6.9%), 1,8-cineole (6.7%), ?-pinene(6.7%) and??-bisabolol (6.6%) as the main constituents while the leaf volatiles obtained by MSD-SPME techniquewere rich in (E)-geranyl acetone (10.5%), (E)-?-ionone (10.3%), camphor (10.2%), 1,8-cineole (9.6%),longiverbenone (7.9%), ?-eudesmol (7.5%), isopropyl myristate (6.7%) and epi-?-bisabolol (6.4%). The root volatileswere rich in longiverbenone (14.1%), (E)-geranyl acetone (9.3%), nonanol (12.1%) and decanol (12.5%). Theenantiomeric distribution of the major volatile constituents was analyzed by using different ?-cyclodextrin chiralcolumns. (1R)-(+)-?-Pinene, (1S)-(-)-?-pinene, (4R)-(+)-limonene, (1R,3S,5R)-(-)-trans-pinocarveol, (1S,2R,4S)-(-)-borneol, (2S)-(-)-?-bisabolol were detected as dominant enantiomers. The lipids extracted from the flower and leafwith Folch method and methylated with BF3 reagent contained common acids: linolenic, linoleic, hexadecanoic acids.Oleic and stearic acids were detected particularly in high amount in the flower lipids.Öğe COMPARATIVE ANATOMICAL INVESTIGATIONS ON SIX ENDEMIC TANACETUM (ASTERACEAE) TAXA FROM TURKEY(Pakistan Botanical Soc, 2016) Tekin, Mehmet; Kartal, CilerTanacetum is one of the large genera, belonging to the Anthemideae tribe of Asteraceae family and has numerous medicinal plants and widely usage in folk medicine. In this study, anatomical features of six endemic species to Turkey viz. Tanacetum albipannosum, T argenteum subsp. argenteum, T cappadocicum, T densum subsp. sivasicum, T haussknechtii and T heterotomum, were investigated for the first time. The specimens were collected from their natural habitats in Sivas province (Turkey). Transverse sections of root, stem, petiole and leaflet were observed under light microscope for various anatomical features. The results showed that, root included periderm in the outer. There were parenchymatous cortex, endodermis and pericycle under the periderm respectively. Primary xylem ridges were triarch in T albipannosum, T densum subsp. sivasicum, T haussknechtii and T heterotomum, pentarch in T cappadocicum and hexarch in T. argenteum subsp. argenteum, and pith was filled with xylem elements. Stem was made up epidermis, parenchymatous cortex, endodermis, vascular bundles and parenchymatous pith from exterior to interior. T heterotomum had a cavity formed by the disintegration of the cells in the center. The amphistomatic leaflets had a single layered epidermis with usually silvery or whitish tomentose indumentum and equifacial mesophyll. Stomata are anomocytic. There were significant difference among examined taxa in respect to contour of petiole, structure of cortex parenchyma and organization of vascular bundles. The anatomical characteristic features of petiole proved to be a useful tool for the taxonomic discrimination of the six studied taxa.Öğe Comparative leaf and peduncle anatomy of four Turkish endemic Onobrychis Mill. taxa(2016) Tekin, Mehmet; Yılmaz, GüldenBu çalışmada, Türkiye'de yayılış gösteren Onobrychis cinsine dâhil olan, sect. Onobrychis'e ait O. quadrijuga, sect. Heliobrychis'e ait O. argyrea subsp. argyrea, sect. Hymenobrychis'e ait O. tournefortii ve O. albiflora taksonlarının yaprak ve pedünkül anatomisi ilk defa incelenmiştir. Yaprak ve pedünkül enine kesitleri üzerinden birçok nitel (kalitatif) ve nicel (kantitatif) karakterler tespit edilerek çalışılmıştır. Sonuçlara göre yapraklar için nicel, pedünkül için ise nitel karakterler, taksonlar arasındaki ayırt edici özellikler açısından önemlidir. Temel olarak, yapraktaki mezofil tabakası kalınlığı, stoma ve epidermis hücrelerinin özellikleri, pedünkülde ise özellikle enine kesit şekli, tüy yüzeyindeki papilla yoğunluğu, boşlukların konumları gibi özellikler incelenen taksonlar arasında dikkate değer şekilde varyasyon göstermektedir.Öğe Composition and potential of Tanacetum haussknechtii Bornm. Grierson as antioxidant and inhibitor of acetylcholinesterase, tyrosinase, and -amylase enzymes(Taylor & Francis Inc, 2018) Yur, Suleyman; Tekin, Mehmet; Goger, Fatih; Baser, Kemal H. C.; Ozek, Temel; Ozek, GulmiraThe phytochemical composition of essential oils and extracts of Tanacetum haussknechtii were investigated with GC-FID-MS and LC-MS/MS techniques and evaluated against oxidation, acetylcholinesterase, tyrosinase, and -amylase enzymes. The major volatile constituents of T. haussknectii were found to be - and -pinene, and borneol. Caffeoylquinic acid derivatives and flavonoids were detected in the aqueous, alcohol, and ethyl acetate extracts. In DPPH assay, the methanol extracts exhibited the highest activity. TEAC assay resulted with superiority of all methanol and the capitula ethyl acetate extract. In -carotene bleaching assay, linoleic acid was the best protected by the ethyl acetate extract of flower. The flower oil inhibited higher acetylcholinesterase activity than the remaining extracts. The flower ethyl acetate extract was found as the most effective inhibitor of -amylase. The herb and the leaf+stem water extracts possess highest inhibitory effect on tyrosinase.Öğe Determination of the Fatty Acid Compositions of Four Onobrychis Species from Turkey(Springer, 2019) Gorgun, Salih; Akpinar, Nukhet; Tekin, Mehmet; Karakas, Hilal[Abstract Not Available]Öğe EMBRYOLOGICAL STUDIES ON SILENE MURADICA SCHISCHK. (CARYOPHYLLACEAE) - A GYNODIOECIOUS SPECIES FROM TURKEY(Polska Akad Nauk, Polish Acad Sciences, Pas Branch Cracow, 2021) Kartal, Ciler; Tekin, MehmetIn this study, female gametophytes of Silene muradica, which is a gynodioecious species, were examined histologically. Buds and blossoms of S. muradica were used as the research material. They were collected in the Sivas province (Turkey) in July 2019, and fixed with ethanol:acetic acid solution (3:1, v/v). Flower parts were dissected under a stereo microscope. They were dehydrated in rising alcohol series and then embedded in Historesin. The sections were taken by a rotary microtome and stained with 0.5% Toluidine blue O. The ovary of S. muradica has three carpels and a single chamber, the ovules are arranged on a central column. The mature ovule is of the campylotropous type, crassinucellate and bitegmig. The megaspore mother cell undergoes regular meiotic division and forms a linear megaspore tetrad after meiosis. The development of the embryo sac is monosporic. The chalazal megaspore is functional and the others degenerate. The mature embryo sac is eight-nucleated and of the Polygonum type. The synergid cells and the egg cell are completely surrounded by the cell wall. Antipodal cells are temporary cells, which degenerate immediately after fertilization. Before fertilization, polar nuclei are fused in the central cell and form the secondary nucleus. The endosperm development is of the nuclear type. Nucellar tissue is permanent and forms perisperm in mature seeds. The embryo development is of the Caryophyllad type. In this study, the development of the female gametophyte of S. muradica, which was determined to be a gynodioecious species, was reported for the first time.Öğe ENDEMİK HEDYSARUM CAPPADOCICUM BOISS. (FABACEAE) TÜRÜNÜN ANATOMİK ÖZELLİKLERİ(2018) Tekin, Mehmet; Akyol, YurdanurHedysarum cappadocicum Boiss. türü İç Anadolu ve Doğu Anadolu’da yayılış gösteren endemik bir türdür. Bu çalışmadakiörnekler Sivas ilinden toplandıktan sonra türün tanımı yapılmıştır. Kök, skapus, petiyol ve yaprakçıkların anatomileriaraştırılmıştır. Türün anatomik özellikleri önceki çalışmaların sonuçlarıyla karşılaştırılmıştır. Türe ait anatomik özelliklerdetaylı bir şekilde ilk olarak bu çalışmada ortaya konulmuştur. Anatomik çalışmalar sonucunda, türün kök enine kesitindesekonder yapı, enine kesitte çıkıntılı skapus, üç köşeli petiyol, yer yer kahverengi renkli tanen içeren hücreli, amfistomatik veanizositik stoma taşıyan yaprakçıklar tanımlanmıştır.Öğe Essential oil characterization of Cousinia sivasica Hub.-Mor. (Asteraceae)(2018) Tekin, Mehmet; Özek, Gülmira; Martin, Esra; Özek, Temel; Yılmaz, Gülden; Başer, Kemal Hüsnü CanIn this study, essential oil properties of an endemic plant of Turkey, Coucinia sivasica Hub.-Mor. were studiedfor the first time. Essential oil of C. sivasica was obtained by using Clevenger apparatus with hydrodistillationtechnique. The essential oil was analyzed by GC-FID and GC/MS techniques on a polar column and total 44compounds were identified representing 98.3%. Fatty acids, their esters, methyl-branched carboxylic acids and aromaticacid esters predominated (66.3%) in the oil with hexadecanoic acid (42.8%), 1-isobutyl 4-isopropyl 3-isopropyl-2,2-dimethyl succinate (12.2%) and methyl salicylate (7.1%) as major constituents.Öğe Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Kocyigit, Umit Muhammet; Eruygur, Nuraniye; Atas, Mehmet; Tekin, Mehmet; Taslimi, Parham; Gokalp, Faik; Gulcin, IlhamiThe research was conducted to separately evaluate and detect the possible in vitro antioxidant, antimicrobial activity of ethanol extracts prepared from aerial parts and roots of Astragalus dumanii and anti-cholinesterase and alpha-glucosidase inhibitory activity from only aerial parts of its The antioxidant capacity was tested by scavenging of DPPH and ABTS free radicals. Compared with the standard antioxidant compound gallic acid; Root and aerial part extract showed lower DPPH radical scavenging activity, however aerial part extract demonstrated higher ABTS radical scavenging activity. The phenolic contents were detected as 5.31 +/- 0.03 and 13.23 +/- 0.05 mg gallic acid equivalent g-1 extract, flavonoid contents were found as 8.26 +/- 0.004 and 7.93 +/- 0.005 mg Qercetin equivalent g-1 extract. In addition, the effects of the extracts obtained from aerial parts of the plant on acetylcholinesterase, butyrylcholinesterase and a-glycosidase enzymes were investigated in vitro and IC50 values were obtained as 1.47, 0.83 and 0.48 mu g mL-1, respectively. When these values were compared with standard substances, it was seen that Astragalus dumanii could be a good enzyme inhibitory agent. Antimicrobial activity of the plant extracts were determined using the microdilution method and the extracts was not observed to have any antimicrobial activities..Öğe Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.(Marmara Univ, Inst Health Sciences, 2023) Eruygur, Nuraniye; Atas, Mehmet; Tekin, Mehmet; Cevik, OzgeObjective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH (1,1'-diphenyl - 2-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AO/EB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.Öğe In Vitro Antioxidant and Enzyme Inhibition Activity of Tanacetum argyrophyllum (K. Koch) Tzvelev var. argyrophyllum Extract(Galenos Publ House, 2022) Eruygur, Nuraniye; Akca, Keyser Taban; Ustun, Osman; Tekin, MehmetObjectives: Tanacetum L. belongs to Asteraceae family and is represented by 46 species in Turkiye. Tanacetum genus is known for its insecticide and insect repellent effect. T. argyrophyllum contains sesquiterpene lactone derivatives. These compounds are responsible for its various activities, especially cytotoxic, antitumor, phytotoxic, antimicrobial, antiviral, and antifungal activity. There are not enough biological activity studies on the plant that are likely to have a wide variety of activities in terms of the compounds it contains. The aim of the present study is to evaluate various biological activities of 80% aqueous methanol extract prepared from aerial parts of T. argyrophyllum (K. Koch) Tzvelev var. argyrophyllum collected from Sivas province of Turkey. Materials and Methods: Antioxidant activity of the methanol extract was determined by 2,2'-diphenyl-1-picryl-hydrazyl and 2,2-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging activity, total phenolic, and total flavonoid content tests. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were investigated via Ellmans spectrometric method. Results: Total phenolic content was found as 71.67 mg/gallic acid equivalent g and total flavonoid content was 25.225 mg/quercetin equivalent g on dry extract weight basis. In this work, AChE, BChE, and alpha-glycosidase enzymes were inhibited by the extract of T. argyrophyllum var. argyrophyllum. IC50 values for these enzymes were found as 266.79 mu g/mL for AChE and 176.91 mu g/mL for BChE. Also, the alpha-glycosidase activity exhibited a dose-dependent manner with increasing concentration. Conclusion: According to the results, T. argyrophyllum var. argyrophyllum can be used as an ingredient of functional foods as well as herbal products for diabetic and Alzheimer's disease patients.Öğe In vivo activity assessment of some Tanacetum species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method(Mashhad Univ Med Sciences, 2018) Ozbilgin, Serkan; Akkol, Esra Kupeli; Oz, Burcin Ergene; Ilhan, Mert; Saltan, Gulcin; Acikara, Ozlem Bahadir; Tekin, MehmetObjective(s): Tanacetum species are traditionally used as insecticide, and externally wound healer as well as for anti-inflammatory and antihistaminic properties. The in vivo wound-healing and anti-inflammatory potential of four Tanacetum species, Tanacetum argenteum (Lam.) Willd. subsp. argenteum (TA), Tanacetum heterotomum (Bornm.) Grierson (TH), Tanacetum densum (Lab.) Schultz Bip. subsp. sivasicum (TD), and Tanacetum vulgare L. (TV) was investigated. Materials and Methods: The chloroform (CHCl3) and methanol: water (80: 20) extracts were prepared from the aerial parts of each plant. For assessment of the wound-healing activity, linear incision on rats and circular excision on mice wound models were used and histopathological analyses were conducted on the tissues treated with the test materials. For the evaluation of the anti-inflammatory activity, Whittle Method based on the inhibition of the acetic acid-induced increase in capillary permeability was used. In order to elucidate the phytochemical contents of the extracts, HPLC profiles of active fractions were screened and quantitative analysis was conducted within the scope of HPLC analysis. Results: The CHCl3 extracts of TD, TA and TV were found to have significant wound healing activity (37.1%, 30.8% and 26.1% tensile strength; 88.05%, 72.93% and 44.88% contraction values, respectively) and anti-inflammatory activities (31.5% and 26.6% inhibition values for TD and TA). Parthenolide content of the CHCl3 extracts of TA, TH and TV were found 242.66 +/- 1.53, 190.16 +/- 5.62 and 177.51 +/- 3.73 mu g/100 mg plant material, respectively. Conclusion: According to the results, the other secondary metabolites present in the aerial parts of the Tanacetum species possibly exerted synergistic effects on the observed healing of the wounds.
