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Inhibitory effect of curcuminoid pretreatments on endogenous dentin proteases
(Japanese Soc Dental Materials Devices, 2018) Seseogullari-Dirihan, Roda; Tekbas Atay, Meltem; Pashley, David Henry; Tezvergil-Mutluay, Arzu
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 0/1 of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhIVIMP-9 were measured using an activity assay. Data were subjected to repeated-measures-ANOVA (alpha=0.05). Gelatinolytic activity was analyzed using zymography. ICTP and CTX release and dry mass loss of curcuminoid-treated groups were significantly lower than the control. Inhibition of rhMMP-9 varied from 29-49% among curcumonoid-treated groups, whereas no inhibition was observed at untreated control (p>0.05). Results were confirmed by zymography. The study showed that the pretreatment of dentin matrices by curcuminoids decreases endogenous protease activity-mediated degradation in dentin.