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    Arginase and Ornithine in Human Benign and Malignant Skin Tumors
    (Walter De Gruyter Gmbh, 2010) Gokmen, Selma Suer; Kazezoglu, Cemal; Aygit, A. Cemal; Yildiz, Reyhan; Cakir, Beyhan; Ture, Mevlut; Gulen, Sendogan
    Objectives: Arginase activity and ornithine concentration have been shown to be elevated in experimentally-induced benign tumors in mice. The aim of the study is to investigate arginase activity and ornithine concentration in human benign and malignant skin tumors and to evaluate their role for prognosis of skin tumors. Patients and Methods: We have investigated arginase activity and ornithine concentration in supernatant of homogenates of benign tumors (nevus) of the skin from 13 patients and of malignant tumors (squamous cell or basal cell carcinomas) from 29 patients. Total arginase activity, ornithine and total protein concentration in supernatant were determined by the methods of Geyer, Chinard and Lowry, respectively. Results: Arginase activity (p=0.006) and ornithine concentration (p=0.007) in nevus were significantly higher than in adjacent normal tissue. There was no significant difference between their levels in basal cell carcinoma and in nevus (p>0.05). There was no significant difference between ornithine concentration in squamous cell carcinoma and in nevus (p>0.05). However, arginase activity in this carcinoma was significantly higher than in nevus (p=0.018). Conclusion: The significant difference between tissue arginase activities in squamous cell carcinoma and in nevus indicates that determination of arginase activity could be useful for prognosis of skin tumors.
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    The Effect of Cisplatin plus Etoposide Therapy on Serum Total and Lipid-Bound Sialic Acid Levels in Patients with Non-small Cell Lung Cancer
    (Turkish Biochem Soc, 2010) Gokmen, Selma Suer; Kazezoglu, Cemal; Tabakoglu, Erhan; Gungor, Ozgul; Altiay, Gundeniz; Ture, Mevlut
    Objectives: To investigate the effect of cisplatin+etoposide therapy on serum total and lipid-bound sialic acid levels in patients with non-small cell lung cancer and evaluate the role of these parameters in the monitoring of the therapy. Patients and Methods: To 18 patients (all men) who are newly diagnosed as nonsmall cell lung cancer, cisplatin was given intravenously (80mg/m(2)) on day 1 and etoposide was given (100mg/m(2)) on day 1-3 to the patients once at an interval of 21 day. Blood samples before the first chemotherapy were compared with those obtained after the second and third chemotherapy. The percent of chemotherapy responses of patients were also calculated. Total and lipid-bound sialic levels were determined by the methods of Warren and Katopodis, respectively. Results: There was a significant decrease in serum lipid-bound sialic acid levels after the second chemotherapy when compared with those before the first chemotherapy (t=2.216, p=0.041). Positive response to cisplatin+etoposide therapy was observed in 88.89% (11.11% of total response, 44.44% of partial response and 33.33% of stable response) of the patients. Progressive disease was established in only 11.11% of the patients. It was found a statistically significant decrease in both serum total (t=2.924, p=0.017) and lipid-bound sialic acid (t=3.635, p=0.005) levels after the third chemotherapy when compared with those before the first chemotherapy. Conclusion: Determination of serum total and lipid-bound sialic acid levels besides routine applications may be useful in the monitoring of cisplatin+etoposide therapy.
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    Effect of L-carnitine on serum paraoxonase, arylesterase and lactonase activities and oxidative status in experimental colitis
    (Walter De Gruyter Gmbh, 2013) Ozgun, Eray; Ozgun, Gulben Sayilan; Eskiocak, Sevgi; Yalcin, Omer; Gokmen, Selma Suer
    Aim: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. We investigated antioxidant L-carnitine effect on activities of paraoxonase 1 enzyme which is also synthesized in colon and oxidative status in experimental colitis. Material and Methods: Wistar albino female rats were divided into four groups randomly: control, colitis, pre-treatment and treatment groups. To induce colitis, single dose of 1 mL acetic acid (%4) was given intrarectally to colitis, pre-treatment and treatment groups. Single dose of 500 mg/kg L-carnitine was given intraperitoneally 1 hour before inducing colitis to pre-treatment group and 24 hours after inducing colitis to treatment group. All groups were sacrificied 48 hours after intrarectally administration. Existence of colitis was confirmed by histopathological changes. Paraoxonase, arylesterase and lactonase activities, total oxidant and antioxidant status, malondialdehyde, and total sialic acid were measured in serum. Oxidative stress index was calculated from the formula. Results: While serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index were significantly elevated, serum paraoxonase, arylesterase and lactonase activities and total antioxidant status were significantly decreased in acetic-acid induced experimental colitis. In acetic-acid induced experimental colitis, L-carnitine caused a significant decrease in serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index but a significant increase in serum arylesterase and lactonase activities of treatment group only. Conclusion: L-Carnitine has an increasing effect on serum arylesterase and lactonase activities and decreasing effect on oxidative stress in acetic acid-induced experimental colitis. Therefore, L-carnitine may be useful for the treatment of inflammatory bowel disease.
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    Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells
    (General Physiol And Biophysics, 2017) Ozgun, Eray; Ozgun, Gulben Sayilan; Tabakcioglu, Kiymet; Gokmen, Selma Suer; Sut, Necdet; Eskiocak, Sevgi
    Paraoxonase-1 (PON1) and paraoxonase-3 (PON3) are anti-atherosclerotic enzymes, synthesized primarily in liver and bound to HDL in circulation. The aim of the present study was to investigate the effects of therapeutic doses of lipoic acid on PON1 and PON3 protein levels, mRNA expression and arylesterase activity in liver. We treated HepG2 cells with 10, 40 and 200 mu M lipoic acid for 72 h. Cell viability was evaluated by 3-(4,5-dimethy1-2-thiazoly1)-2,5-dipheny1-2Htetrazolium bromide assay. PON1 and PON3 protein levels were measured by Western blotting, their mRNA expression was measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. 200 mu M lipoic acid caused a significant increase on PON1 and PON3 protein levels and arylesterase activity as compared with control, 10 mu M and 40 mu M lipoic acid treated cells. 200 mu M lipoic acid also caused a significant decrease on PON1 mRNA expression whereas on a significant increase PON3 mRNA expression as compared with control, 10 mu M and 40 mu M lipoic acid-treated cells. Our study showed that although lip oic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses.
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    The importance of serum total and lipid-bound sialic acid as markers in patients with small cell and non-small cell lung carcinoma
    (Aves Yayincilik, Ibrahim Kara, 2007) Gokmen, Selma Suer; Kazezoglu, Cemal; Tabakoglu, Erhan; Altiay, Guendeniz; Gungoer, Ozgul; Ture, Mevlut
    Objectives: Serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) levels were investigated in patients with small and non-small cell lung carcinoma and their role in discriminating small from non-small cell lung carcinoma and lung carcinoma from healthy individuals was evaluated. Patients and Methods: The study included 159 male patients with non-small cell lung carcinoma (n=102) and small cell lung carcinoma (n=57) who never received chemotherapy and/or radiotherapy and 35 healthy volunteers as controls. Serum TSA and LSA levels were determined by the methods of Warren and Katopodis, respectively. Results: Serum TSA and LSA levels in both patient groups were significantly elevated when compared with controls (p < 0.001), but the patient groups did not differ significantly in this respect. Receiver operating characteristic (ROC) analysis showed that TSA was more specific and LSA was more sensitive in distinguishing patients with non-small cell carcinoma from healthy individuals. On the other hand, LSA was found to be more sensitive in distinguishing patients with small cell carcinoma from healthy controls. Conclusion: Serum total and lipid-bound sialic acid may play an important role as biochemical markers in distinguishing patients with small and non-small cell lung carcinoma from healthy subjects.