The Effect of 5-Fu and Ruxolitinib on Mitochondrial Apoptosis in Glioblastoma U87 Cell Line

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dc.contributor.authorAksu, Gonca
dc.contributor.authorDoğanlar, Oğuzhan
dc.contributor.authorDoğanlar, Zeynep Banu
dc.date.accessioned2021-11-20T10:26:56Z
dc.date.available2021-11-20T10:26:56Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Anabilim Dalıen_US
dc.description.abstractAims: The aim of this study is to carry out the effect of 5-Fluorouracil alone or combined with Ruxolitinib on both apoptosis and JAK/STAT pathway in U87 glioblastoma cells. Methods: We used U87 glioblastoma cell lines as the human brain cancer cells. We treated the cells with 5-Fluorouracil (3.125 ?M-400 ?M) alone and with a combination of Ruxolitinib (100 ?M or 400 ?M of Ruxolitinib with 3.125-25 ?M 5-Fluorouracil), and performed the MTT test for calculating IC50 value. Molecular fluorescence staining was performed with Hoechst and acridine orange/ethidium bromide probes. The alteration in mitochondrial apoptosis and JAK/STAT pathways to drug treatment was analyzed by the qRT-PCR assay. Results: Decrease in cell viability was more prominent in U87 cells treated with a combination of 5-Fluorouracil and Ruxolitinib compared to those treated with 5-Fluoro- uracil alone. In gene expression analysis, apoptosis signals were observed in cells treated with 5-Fluorouracil alone and 5-Fluo- rouracil+Ruxolitinib treatment. Conclusion: Treatment with 5-Fluorouracil alone and 5-Fluorouracil+Ruxolitinib combination increased apoptosis in U87 glioblastoma cells. However, it is difficult to mention an evident difference between treatments. Therefore, further studies are needed.en_US
dc.identifier.dergipark819208en_US
dc.identifier.endpage139en_US
dc.identifier.issn2148-4724
dc.identifier.issn2548-0030
dc.identifier.issue3en_US
dc.identifier.startpage130en_US
dc.identifier.urihttps://dergipark.org.tr/tr/pub/tmsj/issue/57652/819208
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/1373503
dc.identifier.urihttps://hdl.handle.net/20.500.14551/6227
dc.identifier.volume7en_US
dc.language.isoenen_US
dc.publisherTrakya Üniversitesien_US
dc.relation.ispartofTurkish Medical Student Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject5-Fluorouracilen_US
dc.subjectRuxolitiniben_US
dc.subjectglioblastomaen_US
dc.subjectapoptosisen_US
dc.subjectJAK/STAT pathwayen_US
dc.titleThe Effect of 5-Fu and Ruxolitinib on Mitochondrial Apoptosis in Glioblastoma U87 Cell Lineen_US
dc.typeArticleen_US

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