Yazar "Yilmaz, Habibe" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    An intravenous application of magnetic nanoparticles for osteomyelitis treatment: An efficient alternative
    (Elsevier, 2021) Ak, Guliz; Bozkaya, Ummuhan Fulden; Yilmaz, Habibe; Turgut, Ozge Sari; Bilgin, Ismail; Tomruk, Canberk; Uyanikgil, Yigit
    The infection of bone and bone marrow is called osteomyelitis. Treatment is difficult since antibiotics can not reach with enough concentration to the infected area. For the first time in this study, we have developed gentamicin-loaded magnetic gelatin nanoparticles (GMGNPs) for nanocarrier-mediated and magnetically targeted osteomyelitis therapy. Gelatin, genipin, and magnetite were used for preparation of that novel carrier system due to their biodegradable and biocompatible properties. Cross-linking degree of gelatin nanoparticles, concentration of magnetite nanoparticles, and adsorbed drug amount were optimized. Furthermore, nanoparticles were characterized and the drug release profile was determined. The osteomyelitis model was constituted in the proximal tibia of rats. The therapeutic potential of GMGNPs on rats was monitored via X-Ray radiography and hematological and histopathological analyses were performed. According to the results, 110.3 +/- 8.2 mu g gentamicin/mg GMGNPs were used, hydrodynamic size was measured as 253.7 +/- 11.8 nm, and GMGNPs have controlled drug release profile. Based on in vivo and ex vivo studies, after six doses of GMGNPs treatment, abscess began to heal and the integrity of periost and bone began to reconstruct. In conclusion, it can be suggested that GMGNPs could provide efficient therapy for osteomyelitis.
  • Küçük Resim Yok
    Öğe
    A novel second-generation platinum derivative and evaluation of its anti-cancer potential
    (Univ Sao Paulo, Conjunto Quimicas, 2022) Yilmaz, Habibe; Sanlier, Senay Hamarat
    Cisplatin is the primary anti-cancer agent for the treatment of most solid tumors. However, platinum-based anti-cancer chemotherapy produces severe side effects due to its poor specificity. There are a broad interest and literature base for a novel mechanism of action on platinum derivatives. Additionally, combining cisplatin with histone deacetylase inhibitors (HDACi) such as 4-hydroxybenzoic acid derivatives showed promising results in treating solid tumors. Here we aimed to conjugate 4-hydroxybenzoic acid with platinum to obtain a novel platinum derivative that can overcome cisplatin resistance. Cis-4-hydroxyphenylplatinum(II)diamine compound was synthesized under mild conditions and characterized. Cytotoxicity assay was performed on SKOV3-Luc and A549-Luc cells. Hemocompatibility and serum protein binding analysis were performed. Treatment potential was evaluated in xenograft tumor models. Biodistribution was tested on tumor-bearing mice via Pt analysis in organs with ICP-MS, ex vivo. In this study, cis-4-hydroxyphenylplatinum (II) diamine was synthesized with a yield of 62%. The MTT assay on A549-Luc and SKOV3-Luc cell lines resulted in IC50 values of 17.82 and 7.81 mu M, respectively. While tumor growth was continued in the control group, the tumor volume decreased in the treatment group. All results point to the conclusion that the new compound has the potential to treat solid tumors.
  • Küçük Resim Yok
    Öğe
    A pH-Sensitive Chitosan/Oxidized-Kappa-Carrageenan based Nano-Antibiotic for Sustained and Controlled Release of Amoxicillin
    (Taylor & Francis Inc, 2022) Yilmaz, Habibe
    Nowadays antibiotic resistance is one of the most serious problems. Therefore, there is a need for sustained and controlled drug release systems for antibiotics. The aim of this study was to develop a nano-antibiotic system using biocompatible and antimicrobial polymers that are abundant in nature. Frequently used amoxicillin was chosen as the antibiotic. Before nanoparticle synthesis kappa-carrageenan was oxidized and 1.2% carboxyl, and 23.08% aldehyde groups were obtained, as confirmed by Fourier-transform infrared spectroscopy. A combination of polyelectrolyte complexation and ionic gelation was occurred in the nanoparticle synthesis. For the optimal temperature, CaCl2 concentration and polymer ratio of 37 degrees C, 1.5% and 6:1, respectively, the results of hydrodynamic size measurements indicated the size was 414.4 nm. Drug loading encapsulation was successful with an efficiency of 73.28%. Drug release was monitored for 95 h, and drug release was 93% and 85%, respectively, at pH 6.0 and pH 7.4. It is shown by the mathematical models that the drug release profile can be explained by the Higuchi and first-order models since R-2 values were higher than for the other models. As a result, it was observed that the obtained nano-antibiotic delivery system performed a long-term drug release of approximately 4 days in a pH-sensitive manner and would be a promising drug delivery system.