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    The -31 G/C promoter gene polymorphism of survivin in Turkish patients with colorectal cancer
    (Sri Lanka Medical Assoc, 2018) Budak, M.; Bozkurt, C.; Cetin, S. E.; Tuncel, H.
    Introduction Survivin (also known as birc5) is the first protein discovered among the apoptosis-regulating gene family referred to as inhibitor of apoptosis proteins (IAPs). It is expressed and controlled during cellular differentiation and development in human beings. Survivin expression has been shown in a number of cancers and has been associated with cancer development. Objective In our study, we compared normal and tumoural tissue samples, which were obtained from 100 patients diagnosed with colorectal cancer, at Department of Pathology, Istanbul University. Methods The present study employed PCR-RFLP to identify the -31 G/C polymorphism in the promoter region of the survivin gene. Distribution of the survivin polymorphism was compared between control and tumoural tissue samples using the chi-square test. Results Comparison of all samples revealed that there was significant difference in distribution of survivin promoter -31G/C between tumour and normal tissue of the patient group (p<0.05). When genotypes were compared according to gender, there was no statistically significant difference in the distribution of survivin promoter -31G/C in females p=0.420 or males p=0.309. Conclusion A significant difference was seen in distribution of C allele in tumour tissue compared to normal tissue.
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    Mutation detection in the promoter region of survivin gene on N-methyl-N-nitrosourea induced colon tumor model in experiment
    (Comenius Univ, 2014) Budak, M.; Korpinar, M. A.; Kalkan, T.; Tuncel, H.
    Survivin (also known as BIRC5) is one of the first reported inhibitors of apoptosis proteins (IAPs), which is an important family of proteins that regulate apoptosis. It is developmentally regulated and expressed during cell differentiation in humans, mice and rat. Survivin is expressed in a series of human cancers and it has been widely accepted that survivin is strongly related to the onset and development of cancer. In the present study, we tried to determine differences in the promoter region of survivin gene in colon tissue samples from N-methyl-N-nitrosourea (MNU) induced rat colon tumor model and control group. Polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) analysis was used for this aim. No significant differences were found in the promoter region of survivin gene between the normal and tumor tissues (Tab. 2, Fig. 1, Ref. 16). Text in PDF www.elis.sk.