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    The comparison of electron microscopy and scintigraphy in determining the protective effect of dimethylsulphoxide (DMSO) on ischemia/reperfusion injury through pringle maneuver
    (H G E Update Medical Publishing S A, 2001) Hatipoglu, AR; Temiz, E; Yüksel, M; Hoscoskun, Z; Coskun, I; Hüseyinova, G
    Background/Aims: We investigated the role of the electron microscopy and hepatobiliary scintigraphy in determining the effect of DMSO (dimethysulphoxide) and ischemia/reperfusion injury in the liver after the Pringle maneuver. Methodology: Twenty-four rabbits were divided into the following groups; A: Control group, B: Pringle, C: 10mg/kg DMSO, D: 1g/kg DMSO + Pringle. Group A was considered as a control group and only laparotomy was applied. Group B was exposed to Pringle maneuver only. Group C:was given 10mg/kg of DMSO via the vena cava inferior. Group D was given 1g/kg of DMSO- A clamp was fastened for the groups of B, C and D in the 30th minute of the Pringle maneuver and a biopsy was applied five minutes later. Fifteen minutes later a dynamic hepatobiliary scintigraphy was applied. From dynamic images, liver peak time and activity half time of the liver were obtained. Results: It was found that liver peak time and liver activity half time values of the group B, C and D were significantly longer than group A. Liver peak time and liver activity half time values of group B was not different from group C. However, some values of group D were found to be significantly shorter than groups B and C. In the electron microscopy examination, only in group B were some specific degenerative changes observed in the sinusoids. We observed less irreversible changes in group C than in group B. Oh the other hand, the least irreversible changes were in group D. Conclusions: As a conclusion, while electron microscopy is regarded as the gold standard, hepatobiliary scintigraphy may be thought of as an easily applicable: method in determining the ischemic reperfusion injury in the clinical comparison of the protective agents.
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    Evaluating the effect of halothan and the protective effect of catechine on liver parenchym with hepatobiliary scintigraphy
    (Springer-Verlag, 2001) Yuksel, M; Karamanloglu, B; Temiz, E; Salihoglu, YS
    [Abstract Not Available]
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    Evaluation of liver parenchimal damage after pringle manoeuvre and protective effect of DMSO using hepatobiliary scintigraphy
    (Springer Verlag, 1999) Yüksel, M; Hatipoglu, A; Temiz, E; Salihoglu, YS; Hüseyinova, G; Berkarda, S
    [Abstract Not Available]
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    Hepatobiliary scintigraphy for evaluating the hepatotoxic effect of halothane and the protective effect of catechin in comparison with histo-chemical analysis of liver tissue
    (Lippincott Williams & Wilkins, 2002) Karamanlioglu, B; Yüksel, M; Temiz, E; Salihoglu, YS; Çiftçi, S
    Halothane and its metabolites cause liver damage by decreasing liver blood flow and generating free-radical species. Catechin suppresses lipid peroxidation and increases enzyme activity, therefore it seems to be capable of protecting liver parenchyma against tine direct toxic effect of halothane. The aim of this study was to investigate the role of hepatobiliary scintigraphy in detecting liver damage after halothane anaesthesia and the protective effect of catechin in comparison with histo-chemical analysis. Thirty rabbits, divided into three groups (A, controls; B, halothane; and C, catechin+halothane), were investigated. In group A no anaesthesia was administered. Group B only received halothane, while group C was pretreated with catechin and halothane anaesthesia was administered for 2 h. Dynamic scintigrams were taken for 60 min after injecting Tc-99m-mebrofenin, and the time of peak uptake (TPU) and the time for half of the activity to clear from the liver (T-1/2) were calculated. Rabbits were killed, and malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) levels were measured in hepatic tissue. The TPU and T-1/2 values of group A is significantly lower than in groups B and C (P < 0.0002 and P < 0.0002, respectively, for TPU; and P < 0.0002 and P < 0.0003, respectively, for T-1/2). The TPU and T-1/2 values of group B were significantly higher than in group C (P < 0.0003 and P < 0.0003, respectively). The hepatic MDA level of group A was significantly lower than in groups B and C (P < 0.0002 and P < 0.0002, respectively). SOD, GSH-Px and CAT levels of group A were significantly higher than in groups B and C (P < 0.0002, P < 0.0001 and P < 0.003, respectively, for group A vs group B; and P < 0.0005, P < 0.0002 and P < 0.03, respectively, for group A vs group C). The MDA level of group B was significantly higher than that in group C (P < 0.0002). SOD, GSH-Px and CAT levels of group B were significantly lower than in group C (P < 0.0002, P < 0.0002 and P < 0.003, respectively). According to these results, we suggest that catechin protects liver parenchyma against the toxic effect of halothane and its metabolites, and that, compared to invasive histo-chemical analysis, hepatobiliary scintigraphy is a useful and alternative noninvasive method for detecting the protective effect of catechin on liver parenchyma after halothane anaesthesia. ((C) 2002 Lippincott Williams & Wilkins).
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    The role of hepatobiliary scintigraphy in the evaluation of the protective effects of dimethylsulphoxide in ischaemic/reperfusion injury of liver
    (Lippincott Williams & Wilkins, 2000) Yüksel, M; Hatipoglu, A; Temiz, E; Salihoglu, YS; Hüseyinova, G; Berkarda, S
    Liver ischaemia may lead to parenchymal damage depending on the duration of the ischaemia. Dimethylsulphoxide (DMSO), a well-known radical oxygen scavenger, is a protective agent against ischaemia/reperfusion injury. In this study we aimed to investigate the role of hepatobiliary scintigraphy (HBSc) in detecting the protective effect of DMSO. Eighteen rabbits, in three groups of six, were injected with 37 MBq technetium-99m-mebrofenin via the ear veins. Dynamic scintigrams were taken for 60 min (1 frame/min). In group A, HBSc was performed without any surgery. In groups B and C the Pringle manoeuvre (PM) was applied for 30 min, and tissue specimens for electron microscopy were taken from the liver parenchyma 5 min after the end of the PM. In addition, in group C 1 g/kg DMSO was injected into each rabbit 5 min before application of the PM. HBSc was then performed in groups 5 and C. From the dynamic images time-activity curves (TACs) were obtained for each group, and the time of peak uptake (TPU) and time for half of the activity to clear from the liver (T 1/2) were calculated. The TPU and T 1/2 of group B were significantly longer than those of groups A and C (P <0.0005 and P < 0.005 for TPU, and P<0.0005 and P<0.02 for T 1/2 respectively). The TPU and T 1/2 of group C were significantly longer than those of group A (P <0.005 and P<0.02, respectively). While the electron microscopic images in group C showed reversible changes, those in group B showed both irreversible and reversible changes. The electron microscopic findings of groups B and C confirmed the scintigraphic findings. In conclusion, HBSc might be used as a practical quantitative method for detecting the protective effects of DMSO. However, its clinical value should be evaluated by further studies with human subjects. ((C) 2000 Lippincott Williams & Wilkins).