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Öğe ARSENIC EFFECTS ON LIPID PEROXIDATION AND ANTIOXIDATIVE ENZYME ACTIVITIES IN BARLEY CULTIVARS ROOTS(Parlar Scientific Publications (P S P), 2018) Peksen, Sezgi; Sanal, FilizAntioxidant enzymes are related to resistance to various abiotic stresses including heavy metal stress. In this study, physiological parameters of Sladoran and Balkan 96 species of barley (Hordeum vulgare) Arsenic (As) such as germination rate, root length and dry and wet weight were evaluated in 10 mu M (low and non-toxic dose) and 50 mu M (high and toxic dose) two concentrations, its effect on antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx), Guaiacol peroxidase (GPx) in root tissue of arsenic and effect on lipid peroxidation which is an important determinant of oxidative stress were evaluated, the effect of barley on arsenic toxicity was analyzed in biochemical sense. Parallel to dose increase, arsenic inhibits root growth in both species of barley, dry and wet weight decreased distinctly, it caused lipid peroxidation in the roots. Besides, against oxidative stress caused by arsenic, barley reacted in root tissue with changes in antioxidative enzyme activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx), Guaiacol peroxidase (GPx). While there was a significant (p<0.005) decrease in activities of APx and CAT enzyme activities, there was increase in SOD enzyme activities. There was no significant change in GPx enzyme activity. The results of the present experiment coincided with the conclucion that As may be involved in the metabolic or physiological changes in plants.Öğe Effects of Arsenate and Arsenite on Germination and Some Physiological Attributes of Barley Hordeum vulgare L.(Springer, 2014) Sanal, Filiz; Seren, Gulay; Guner, UtkuArsenic (As) is toxic to plants and animals. We tested the effects of arsenite and arsenate (0-16 mg/L) on seed germination, and on relative root and shoot length, alpha-amylase activity, reducing sugars and soluble total protein contents, and malondialdehyde content in barley seedlings. We also measured As accumulation in barley stems and roots. The alpha-amylase activity, relative root and shoot length, and seed germination decreased with increasing concentrations of arsenate and arsenite. The reducing sugars content in barley seedlings increased after 4 days of growth on media containing As. In general, the protein content in roots and seedlings decreased with increasing doses of As. Arsenic in the tissues was quantified by hydride generation-atomic absorption spectrophotometry. To confirm the accuracy of the method, we analyzed the certified reference material WEPAL-IPE-168. The limit of detection was 1.2 mu g/L and the relative standard deviation was < 2.0 %.Öğe MESNA HAS ANTIOXIDANT POTENTIAL ON INTESTINE, LIVER, KIDNEY AND LUNG INJURY INDUCED BY INTESTINAL ISCHEMIA/REPERFUSION(Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2022) Sanal, Filiz; Bakar, Elvan; Inan, Mustafa; Ulucam, Enis; Cerkezkayabekir, AyegulAn ischemia/reperfusion (IR) injury of small intestine is a serious and common condition that is a result of the blockage of the superior mesenteric artery (SMA) due to some significant clinical problems. In the present study, we evaluated the effect of Mesna on systemic injury induced by IR in small intestine and liver, kidney and lung of rats. Thirty-two Wistar albino female rats were randomly divided into four groups as control, ischemia, IR (Sham) and IR+Mesna. Ischemia period was executed by clamping SMA for 2 h and after when reperfusion was permitted by removing the clamp from SMA for 2 h. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities were measured spectrophotometrically in the tissues. Microscopic examination was performed with Hematoxylin-Eosin (H&E) staining for light microscopy and double staining (Uranyl acetate and Reynolds'lead citrate) for electron microscopy. Oxidative damage was determined in ischemia (p < 0.05) and IR groups (p < 0.05) by Chiu score in small intestine and also antioxidant enzyme activities in all groups. Significant recovery in SOD, GPx and CAT activities in IR+Mesna group was determined but the effect of oxidative damage was not reduced significantly histopatologically. Mesna treatment caused recovery in SOD, GPx and CAT activities but not achieved an improvement in the histopathologic findings in level of light microscopy in IR damages but contains the signs of improving at electron microscopy level in Mesna dose and application time of IR.Öğe Mezenkimal Kök Hücrenin Bağırsak İskemi/Reperfüzyon Hasarında Antioksidan Kapasite Üzerine Etkisi(2014) Kocagümüş, Ayşe Meltem; Karaöz, Erdal; Bakar, Elvan; Sanal, Filiz; Çerkezkayabekır, Aysegül; Uluçam, Enis; İnan, Mustafa[Abstract Nıt Available]Öğe Preliminary characterization of crude inulinase activity of aspergillus wentii(Trakya Üniversitesi Fen Bilimleri Enstitüsü, 2015) Sanal, Filiz; Karatop, RumeysaThis study aims to perform a partial characterization of the crude enzymatic extracts of Aspergillus wentii inulinase produced by using the Jerusalem artichoke as the only carbon source in a production medium, which is a new source for obtaining inulinase. The screening medium that contained inulin as the only carbon source used by Derycke and Wandamme (1984) was redesigned to include Jerusalem artichoke powder instead of inulin and was used for the inulinase production of A. wentii. The crude inulinase produced by A.wentii was preliminarily characterized for its activity and stability. Optimum pH, temperature and incubation times for the crude enzymatic extract were determined as pH 6.0, 35°C and 10 min, respectively. The Km and the Vmax values were calculated as approximately 1×10-4 M, and 6.134 mmol/ml/min for A. wentii inulinase, respectively. The enzyme activity was observed to be stable at the intervals of pH 3.0-6.0 (96.8%), to maintain its activity until 50°C, and to have 62.3% relative activity at 80°C. The results confirm that A. wentii inulinase can be an enzyme with industrial treatments due to its relationship with its substrate and its good pH stability and thermal stability. A. wentii inulinase can be a new and usable source for preparation of pure fructose from inulin-containing agricultural products.Öğe PRELIMINARY CHARACTERIZATION OF CRUDE INULINASE ACTIVITY OF ASPERGILLUS WENTII(2015) Karatop, Rumeysa; Sanal, Filizİnülinazlar fonksiyonel besin maddeleri olarak kullanılan inülin ve fruktooligosakkaritlerin enzimatik hidrolizi ile fruktoz şurubu üretiminde kullanılabilen önemli bir enzim grubudur. Bu çalışmada inulinaz eldesi için yeni bir kaynak olan ve üretim ortamında tek karbon kaynağı olarak yer elması kullanılarak üretilen Aspergillus wentii inulinazının kaba enzimatik ekstraktlarının kısmi karakterizasyonunun gerçekleştirilmesi hedeflendi. Derycke ve Wandamme (1984)'ın kullandığı, karbon kaynağı olarak sadece inülin içeren tarama medyumu, inülin yerine yer elması tozu kullanılarak yeniden düzenlendi ve A. wentii'nin inülinaz üretimi için kullanıldı. A. wentii inulinazı'nın aktivite ve stabilite açısından kısmi karakterizasyonu gerçekleştirildi. Kaba enzimatik ekstrakt için optimum pH, sıcaklık ve inkübasyon süresi sırası ile pH 6,0, 35?C ve 10dk olarak belirlendi. A. wentii inülinazı için Km değeri yaklaşık 1×10-4M, Vmax değeri ise 6,134mmol/ml/dk olarak hesaplandı. Enzimin pH 3,0-6,0 aralığında aktivitesinin kararlı olduğu (%96,8) ve 50?C ye kadar aktivitesini koruduğu ve 80?C'de %62,3 bağıl aktiviteye sahip olduğu görüldü. Elde edilen sonuçlar A. wentii inulinazının subtratı ile olan ilişkisi, iyi bir pH ve termal kararlılığa sahip olması göz önüne alındığında endüstriyel olarak kullanılabilir bir enzim olduğunu doğrulamaktadır. A. wenti inulinazı inulin içeren tarımsal ürünlerden saf fruktoz şurubu hazırlanması için yeni ve uygun bir kaynak olabilir.
