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Öğe Beneficial Effects of Quercetin on the Zone of Stasis in an Experimental Burn Model(Aves, 2018) Orhan, Erkan; Sahin, Ertan; Metin, Melike Sapmaz; Tarladacalisir, Yeter TopcuObjective: In burn injury, the zone of stasis determines the width and depth of the necrosis. Our aim is to show the effectiveness of quercetin on the viability within the zone of stasis in burns of rats. Materials and Methods: Forty-eight rats were divided into three groups. The rats in Group 1 (control group) were only applied the comb burn model; the rats in Group 2 (post-burn group) were administered 50 mg/kg of quercetin intraperitoneally, every day after the burn procedure until euthanasia; and the rats in Group 3 (pre-burn group) were administered 50 mg/kg of quercetin intraperitoneally, every day for 7 days before and after the burn procedure until euthanasia. Results: The living tissue calculated was 85.41% (+/- 14.06) in Group 3, 40.37% (+/- 9.75) in Group 2, and 16.81% (+/- 9.4) in Group 1. The level of apoptosis was 30.0 (+/- 10.8) in Group 3, 33.8 (+/- 08.7) in Group 2, and 37.4 (+/- 11.5) in Group 1. The level of autophagy was 49.50 (+/- 8.58) in Group 3, 27.17 (+/- 5.53) in Group 2, and 21.00 (+/- 5.66) in Group 1. All the differences between the groups were statistically significant (p< 0.01). Conclusion: Quercetin reduces apoptosis and increases autophagy, thereby increasing tissue viability in the zone of stasis of burn injury.Öğe Polysubstituted pyrrolidines linked to 1,2,3-triazoles: Synthesis, crystal structure, DFT studies, acid dissociation constant, drug-likeness, and anti-proliferative activity(Elsevier, 2020) Ince, Tuncay; Serttas, Riza; Demir, Bunyamin; Atabey, Hasan; Seferoglu, Nurgul; Erdogan, Suat; Sahin, ErtanNovel pyrrolidines linked to 1,2,3-triazole derivatives, dimethyl 1-(2 (4-R-1H 1,2,3 triazol-1-yl)-acetyl)-5,5-diphenylpyrrolidine-2,4-dicarboxylate, were synthesized in the presence of sodium ascorbate and copper (II) sulfate pentahydrate by click chemistry in excellent 89-95% yield. The H-1 NMR, C-13 NMR, DEPT, COSY, HMQC, FT-IR, HRMS and elemental analysis techniques were used for structural characterization. A single crystal X-ray diffraction study was performed in order to determine the stereochemistry of the compounds. The optimization geometry of the compounds was obtained by performing density functional theory. However, the global reactivity parameters were estimated from frontier molecular orbitals. The calculated global reactivity parameters showed that the most reactive compound was 4e and the least reactive compounds were 4a, 4c and 4d. The acid dissociation constants were determined using potentiometric titration in 20% (v/v) dimethyl sulfoxide-water hydro-organic solvent at 25 +/- 0.1 degrees C, and four dissociation constants relating nitrogen atoms were obtained for each ligand. Six synthesized compounds were screened for their in vitro anti-proliferative activities against human prostate cancer cells, all of which significantly reduced cell proliferation. One of the compounds 4d showed much more anti-proliferative potential relative to the others, delaying the cells at the DNA synthesis phase. The drug-likeness model score (DLS) calculations for all the synthesized compounds using MolSoft websites supported the experimental results of anti-proliferative bioactivity study, and 4d was found to be maximum DLS value of 1.01. (C) 2020 Elsevier B.V. All rights reserved.Öğe Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood serum(Taylor & Francis Ltd, 2019) Orhan, Erkan; Gunduz, Ozgur; Kaya, Oktay; Oznur, Meltem; Sahin, ErtanThe aim of this study was to show whether the protective effect of remote ischemic preconditioning (RIPC) on flaps can be transferred among different individuals with the transfusion of blood serum. Blood serum was taken from rats without any procedure (Group x), rats 1 hour (Group y) and 24 hours (Group z) after performing RIPC and the remaining rats were divided into six groups. While the random pattern skin flap was performed only in the back region in Group 1, and it was performed 1 hour (Group 2) and 24 hours (Group 3) after induction RIPC. Flap surgery was performed after the intravenous injection of serum obtained from Group x in Group 4, from Group y in Group 5, and from Group z in Group 6. After 7 days, the ratios of viable areas in the flaps of the remaining rats were calculated. When the viable area ratios in the flaps to the whole flap area were calculated, it was found out that the viable area ratios in Group 2 (61.6%), Group 3 (75.6%) and Group 6 (74.2%) were statistically significantly higher compared to Group 1 (51.5%), Group 4 (52.6%) and Group 5 (58.7%), that viable area ratios in Groups 3 and 6 were statistically significantly higher compared to Group 2, and that there was no difference between Groups 3 and 6. This study showed that RIPC forms a protective effect on the flaps and that this effect could be transferred among individuals with blood serum.