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Öğe Beneficial effects of nontoxic ozone on H2O2-induced stress and inflammation(Canadian Science Publishing, 2016) Kucukgul, Altug; Erdogan, Suat; Gonenci, Ramazan; Ozan, GoncaIn this study, the anti-oxidant and anti-inflammatory efficacy of ozone oxidative preconditioning (OOP) were investigated on hydrogen peroxide (H2O2)-induced human lung alveolar cells. In MTT and trypan blue viability tests, while 100 mu mol/L H2O2 caused a 17.3% and 21.9% decrease in the number of living cells, respectively, ozone at 20 mu mol/L regenerated cell proliferation and prevented 9.6% and 11.0% of cell loss, respectively. In addition, H2O2 decreased the transcription levels of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) 5.43-, 2.89-, and 5.33-fold, respectively, while it increased Bax, NF-kappa beta, TNF-alpha, and iNOS expression 1.57-, 1.32-, 1.40-, and 1.41-fold, respectively. Ozone pretreatment, however, increased CAT, GPx, and SOD transcription levels 7.08-, 5.17-, and 6.49-fold and decreased Bax, NF-kappa beta, TNF-alpha, and iNOS transcriptions by 1.25-, 0.76-, 3.63-, and 7.91-fold, respectively. Moreover, intracellular glutathione (GSH) level and SOD activity were decreased by 46.2% and 45.0% in the H2O2 treatment group, and OOP recovered 58.5% and 20.1% of the decreases caused by H2O2. H2O2 also increased nitrite levels 7.84-fold, and OOP reduced this increase by half. Consequently, OOP demonstrated potent anti-oxidant and anti-inflammatory effects on in vitro model of oxidative stress-induced lung injury.Öğe The Effects of Licorice (Glycyrrhriza glabra) Root on Performance, Some Serum Parameters and Antioxidant Capacity of Laying Hens(FACTA-FUNDACIO ARNCO CIENCIA TECNOLOGIA AVICOLAS, 2018) Dogan, Canogullari S.; Baylan, Mikail; Kucukgul, Altug; Bulancak, Ayşen; Erdoğan, ZeynepThe current study was conducted to determine the effects of the licorice root (Glycyrrhriza glabra) in laying hens diets on performance, egg cholesterol, some plasma parameters and antioxidant capacity. One hundred, 40-week old laying hens were divided into four groups, each group consisted of 25 hens and were placed in individual cages. The mean of the initial body weight of laying hens was 1829.18 +/- 9.595 g. Commercial laying hen diet was supplemented with 0, 0.5, 1.0 and 2.0% levels of licorice root powder and four different dietary groups were formed. From the experimental findings, it was ascertained that the licorice root supplementation had no significant effect on egg weight and feed conversion ratio (p>0.05), but feed consumption decreased with increasing licorice root (p<0.05).Egg yield was recorded as 88.94%, 89.56%, 86.82% and 85.02% in the groups of 0, 0.5, 1.0 and 2.0, respectively (p<0.05).Plasma low density lipoprotein (LDL) and egg yolk cholesterol level decreased with the addition of licorice root, while plasma high density lipoprotein (HDL) level was increased with licorice root addition (p<0.05). Licorice root addition had a positive effect on total antioxidant capacity (TAS) of plasma. It was determined that the total antioxidant capacity was increased by increasing amount of licorice root. From the overall findings, it can be concluded that licorice root could be used as a feed additive without any adverse effect on performance. It has been demonstrated that the licorice root enables the production of functional eggs.Öğe Inhibition of Cigarette Smoke Induced-inflammation and Oxidative Damage by Caffeic Acid Phenethyl Ester in A549 Cells(Asian Journal Pharmaceutics, 2016) Kucukgul, Altug; Erdogan, SuatIntroduction: The main objective of this study was to evaluate the effect of antioxidative and antiapoptotic effects and underlying molecular mechanisms of caffeic acid phenethyl ester (CAPE) on human lung epithelial cells exposed to cigarette smoke (CS). Materials and Methods: Human alveolar epithelial A549 cells were exposed to CS and treated with various concentrations of CAPE for 24 h, and their effective concentrations were identified by cell viability assay (MTT). Antioxidant and anti-inflammatory effect of CAPE on nuclear erythroid related factor-2 (Nrf2) and nuclear factor-kappa beta (NF-kappa beta) protein levels were analyzed by Western blotting. Furthermore, caspase 8 gene expression level was analyzed by reverse transcription-quantitative polymerase chain reaction. Results: Low concentration CAPE pretreatment rescued 52% of CS-exposed A549 cells from death. CS upregulated gene expression level of caspase 8 by 4.28 fold. However, 2.5 mu M CAPE pretreatment increased caspase 8 level by 52%. CS exposure also elevated NF-kappa beta (p65) protein level by 70%, however, CAPE pretreatment significantly reversed this activation. While CS exposure decreased Nrf2 protein levels by 48% as compared with the control group, CAPE pretreatment increased Nrf2 protein level two folds approximately according to CS group. Discussion: CAPE markedly decreased inflammatory transcription factor NF-kB and increased antioxidant response element Nrf2 protein expression levels in CS-exposed human alveolar cells. According to the data obtained from this study, CAPE could be used as a strategic alternative to support treatment of inflammatory and oxidative stress-induced lung diseases.Öğe Low concentration of oleic acid exacerbates LPS-induced cell death and inflammation in human alveolar epithelial cells(Taylor & Francis Inc, 2017) Kucukgul, Altug; Erdogan, SuatPurpose: The current study aimed to investigate in vitro effects of oleic acid on lipopolysaccharide (LPS)-induced acute lung injury in the human lung epithelial cells (A549). Materials and Methods: The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests. Selected gene expression levels were analyzed by Real-Time Quantitative-Polymerase Chain Reaction (RT-qPCR). Results: 24hours of LPS (100ng/mL) exposure decreased the cells' viability by 44.6% compared to untreated control. Low concentration (2.5nM) of oleic acid slightly suppressed the cell survival by 9.1% analyzed 24hours after incubation. However, oleic acid pretreatment before LPS exposure significantly increased cell survival loss to 63.9%. LPS exposure decreased the expressions of catalase (CAT) and glutathione peroxidase (GPx) mRNA levels by 2.8 and 2.5 fold, respectively. Moreover, pretreatment of the cells with oleic acid strengthened LPS-decreased expressions of CAT and GPx genes by 3.5 and 6.7 fold, respectively. The mRNA expressions of superoxide dismutase (SOD), induced nitric oxide synthase (iNOS), interleukin-1 beta, IL-12, COX-2, caspase-3 and caspase-8 were increased by 2.4, 2.2, 2.2, 2.3, 3.0, 2.6, and 2.5 fold, respectively, by LPS, and oleic acid pretreatment significantly potentiated the effect of LPS. Conclusion: Oleic acid worsens LPS-induced cell death by potentiating oxidative stress and inflammation in A549 lung epithelial cells.Öğe PERFORMANCE, EGG QUALITY AND SERUM PARAMETERS OF JAPANESE QUAILS FED DIET SUPPLEMENTED WITH SPIRULINA PLATENSIS(Parlar Scientific Publications (P S P), 2016) Dogan, Sibel Canogullari; Baylan, Mikail; Erdogan, Zeynep; Akpinar, Gulsen Copur; Kucukgul, Altug; Duzguner, VesileThis experiment was conducted to evaluate the influence of Spirulina platensis on growth performance, egg quality and some serum parameters of laying Japanese quails. For this experiment, 100 ten weeks-old, female Japanese quails (Coturnix coturnix Japonica) with similar body weight were caged individually and were randomly divided into four groups of 25 quails each. Quails were fed diets supplemented with 0 (control), 0.5, 1.0 and 2.0% Spirulina platensis for 8 weeks. Spirulina platensis addition did not affect feed conversion ratio, feed intake, egg production, egg weight, shape index, eggshell thickness and haugh unit, significantly (P>0.05). However, there were significant differences (P<0.05) in final body weight, yolk index, albumen index and eggshell weight of experimental groups. There were significant differences (P<0.05) between groups in low density lipoprotein (LDL cholesterol) and high density lipoprotein (HDL cholesterol) concentrations. The LDL cholesterol concentration decreased while HDL cholesterol concentration increased with the increased supplementation of Spirulina platensis. Spirulina platensis supplementation also decreased plasma total cholesterol and trigyliceride levels between groups numerically but not statistically (P>0.05). The mean egg yolk cholesterol levels dropped by 19.65 and 18.93% in the 1.0 or 2.0% Spirulina platensis supplemented groups compared with control group. In conclusion, Spirulina algae can be used safely in laying quails diets with important effects on serum parameters and egg yolk cholesterol.
