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Öğe Anti-apoptotic effects of curcumin on cadmium-induced apoptosis in rat testes(Sage Publications Inc, 2012) Aktas, Cevat; Kanter, Mehmet; Erboga, Mustafa; Ozturk, SamilCadmium (Cd) is one of the environmental pollutants affecting various tissues and organs including testis. The aim of this study was to investigate the anti-apoptotic effects of curcumin (Cur) on Cd-induced apoptosis in rat testes. The rats were randomly allotted into one of three experimental groups: control, Cd treated and Cd treated with Cur; each group contained 10 animals. The control group received 2 ml/day of dimethyl sulfoxide (DMSO). To induce toxicity, Cd (1 mg/kg body weight) was dissolved in normal saline and subcutaneously injected into rats for 4 weeks. The rats in Cur-treated group was given a daily dose of 100 mg/kg of Cur for 4 weeks. To date, no examinations of the anti-apoptotic properties of Cur on Cd-induced apoptosis in rat testes have been reported. The mean seminiferous tubule diameter, mean testicular biopsy score values and serum testosterone levels were significantly decreased in Cd-treated groups were compared to the control group. Furthermore, the Cur-treated animals showed an improved histological appearance and serum testosterone levels in Cd-treated group. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling in testis tissues of the Cd-treated group with Cur therapy. The present study showed that Cur treatment protected testes against toxic effects of Cd. We believe that further preclinical research into the utility of Cur may indicate its usefulness as a potential treatment on the spermatogenesis after testicular injury caused by Cd-treated rats.Öğe Anti-inflammatory and antioxidant effects of infliximab in a rat model of intestinal ischemia/reperfusion injury(Sage Publications Inc, 2012) Pergel, Ahmet; Kanter, Mehmet; Yucel, Ahmet Fikret; Aydin, Ibrahim; Erboga, Mustafa; Guzel, AhmetThe aim of this study was to investigate the possible protective effects of infliximab on oxidative stress, cell proliferation and apoptosis in the rat intestinal mucosa after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group comprised 10 animals. Sham group animals underwent laparotomy without I/R injury. I/R groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation occurred, which was followed by 1 hour of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and intestinal tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no biochemical and histopathological changes have been reported regarding intestinal I/R injury in rats due to infliximab treatment. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased reduced superoxide dismutase and glutathione peroxidase enzyme activities in intestinal tissues samples. I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury, inhibiting I/R-induced apoptosis, and cell proliferation. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on the experimental intestinal I/R model of rats.Öğe Anti-inflammatory and antioxidant effects of infliximab on acute lung injury in a rat model of intestinal ischemia/reperfusion(Springer, 2012) Guzel, Ahmet; Kanter, Mehmet; Guzel, Aygul; Pergel, Ahmet; Erboga, MustafaThe purpose of this study was to investigate the role of infliximab on acute lung injury induced by intestinal ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group contain 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered by intravenously. All animals were sacrificed at the end of reperfusion and lung tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no more biochemical and histopathological changes on intestinal I/R injury in rats by infliximab treatment have been reported. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased of reduced superoxide dismutase, and glutathione peroxidase enzyme activities in lung tissues samples. Intestinal I/R caused severe histopathological injury including edema, hemorrhage, increased thickness of the alveolar wall and a great number of inflammatory cells that infiltrated the interstitium and alveoli. Infliximab treatment significantly attenuated the severity of intestinal I/R injury. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase and arise in the expression of surfactant protein D in lung tissue of acute lung injury induced by intestinal I/R with infliximab therapy. It was concluded that infliximab treatment might be beneficial in acute lung injury, therefore, shows potential for clinical use. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects in acute lung injury induced by intestinal I/R.Öğe Antiapoptotic and proliferative activity of curcumin on ovarian follicles in mice exposed to whole body ionizing radiation(Sage Publications Inc, 2012) Aktas, Cevat; Kanter, Mehmet; Kocak, ZaferThe aim of this study was to evaluate the antiapoptotic and proliferative activity of curcumin (Cur) on the ovarian follicles in mice exposed to whole body ionizing radiation (Rd). The mice were exposed to 8.3 gray whole body Rd, and Cur groups were given as a daily dose of 100 mg/kg of Cur for 10 days (10 days before Rd). The ovaries were collected 3 and 12 h after irradiation. To date, no such studies have been performed on antiapoptotic and proliferative activity of Cur on the ovarian follicles in mice exposed to whole body Rd. Analysis of mice ovary after exposure to Rd by terminal-deoxynucleotidyl-transferase-mediated dUTP nick end labeling showed that there were apoptotic cells both in the follicular wall and the antrum, and that the number of follicles showing early atresic features was high 3 h after Rd. On the other hand, analysis of mice ovary 12 h after exposure to Rd showed that the number of follicles containing apoptotic cells with advanced atresic features was significantly higher when compared to the 3-h Rd exposure group. The proliferating cell nuclear antigen-positive granulosa cells were decreased in association with follicular atresia. The groups given treatment were observed to have some benefit from Cur against the damage caused by Rd. The results of this study demonstrate that Cur prevents follicular atresia in Rd-induced apoptosis in ovarian follicles.Öğe Antiapoptotic effect of l-carnitine on testicular irradiation in rats(Springer, 2010) Kanter, Mehmet; Topcu-Tarladacalisir, Yeter; Parlar, SuleWe evaluated the effects of l-carnitine on apoptosis of germ cells in the rat testis following irradiation. Male Wistar rats were divided into three groups. Control group received sham irradiation plus physiological saline. Radiotherapy group received scrotal gamma-irradiation of 10 Gy as a single dose plus physiological saline. Radiotherapy + l-carnitine group received scrotal irradiation plus 200 mg/kg intraperitoneally l-carnitine. Twenty-four hours post-irradiation, the rats were sacrificed and testes were harvested. Testicular damage was examined by light and electron microscopy, and germ cell apoptosis was determined by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) technique. Morphologically, examination of irradiated testis revealed presence of disorganization and desquamation of germinal cells and the reduction in sperm count in seminiferous tubule lumen. Under electron microscopy, the morphological signs of apoptosis were frequently detected in spermatogonia. Apoptotic spermatogonia showed the marginal condensation of chromatin onto the nuclear lamina, nucleus and cytoplasm shrinkage and still functioning cell organelles. TUNEL-positive cells were significantly more numerous in irradiated rats than in control rats. l-carnitine treatment significantly attenuated the radiation-induced morphological changes and germ cell apoptosis in the irradiated rat testis. In conclusion, these results suggested that l-carnitine supplementation during the radiotherapy may be beneficial for spermatogenesis following testicular irradiation by decreasing germ cell apoptosis.Öğe Antioxidant and anti-apoptotic effects of onion (Allium cepa) extract on doxorubicin-induced cardiotoxicity in rats(Wiley, 2013) Alpsoy, Seref; Aktas, Cevat; Uygur, Ramazan; Topcu, Birol; Kanter, Mehmet; Erboga, Mustafa; Karakaya, OsmanThe aim of this study was to investigate the antioxidant and anti-apoptotic effects of onion (Allium cepa) extracts (ACE) on doxorubicin (DOX)-induced cardiotoxicity. The rats in the ACE-pretreated group were given a daily dose of 1 ml ACE for 14 days. To induce cardiotoxicity, DOX (30 mg kg-1 body weight) was injected intraperitoneally by a single dose and the rats were sacrificed after 48 h. To date, no such studies have been performed on the cardioprotective and anti-apoptotic potential of ACE on DOX-induced cardiotoxicity. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling in cardiomyocytes of the DOX-treated group with ACE therapy. The DOX-treated with ACE groups showed a significant decrease in malondialdehyde levels, and increased activities of superoxide dismutase, glutathione and glutathione peroxidase in comparison with the DOX-treated group. Creatine kinase, creatine kinase MB, lactate dehydrogenase activities and cardiac troponin I levels were significantly decreased in the DOX + ACE group in comparison with the DOX group. These biochemical and histological disturbances were effectively attenuated on pretreatment with ACE. The present study showed that ACE may be a suitable cardioprotector against toxic effects of DOX. Copyright (C) 2011 John Wiley & Sons, Ltd.Öğe Antioxidative, antiapoptotic, and proliferative effect of curcumin on liver regeneration after partial hepatectomy in rats(Sage Publications Inc, 2015) Toydemir, Toygar; Kanter, Mehmet; Erboga, Mustafa; Oguz, Serhat; Erenoglu, CengizThe aim of the present study was to assess the influence of curcumin on liver regeneration after partial hepatectomy (PH) in rats. A total of 24 male Sprague Dawley rats were divided into three groups: sham-operated (SH), PH, and PH+curcumin; each group contains eight animals. The rats in curcumin-treated groups were given curcumin (in a dose of 100mg/kg body weight) once a day orally for 7days, starting 3days prior to hepatectomy operation. At 7days after resection, liver samples were collected. The malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) levels were estimated in liver homogenates. Moreover, histopathological examination, mitotic index (MI), proliferating cell nuclear antigen labeling, proliferation index (PI), transferase-mediated 2-deoxyuridine, 5-triphosphate nick end-labeling assay, and apoptotic index (AI) were evaluated at 7days after hepatectomy. As a result, curcumin significantly increased MI and PI and significantly decreased AI in PH rats. Additionally, curcumin remarkably inhibited MDA elevation, restored impaired antioxidant SOD activity and GSH level and also attenuated hepatic vacuolar degeneration and sinusoidal congestion. These results suggested that curcumin treatment had a beneficial effect on liver regenerative capacity of the remnant liver tissue after hepatectomy, probably due to its antioxidative, antiapoptotic, and proliferative properties.Öğe Balb/C türü dişi farelerde uniseksüel gruplamanın östrus siklusu üzerine etkileri(2010) Kanter, Mehmet; Metin, Melike Sapmaz; Ömürlü, İmran KurtSosyal çevreye ait uyarıcılar farelerde östrus siklusunun frekansını ve düzenini etkiler. Kalabalık halde bırakılan dişi farelerin çoğu spontan yalancı gebelik ya da persis-tent diöstrus gösterme eğilimindedir. Bu yüzden dişi fareler arasındaki ilişkiler onların kafesleme koşullarını belirleyen önemli bir faktör olarak dikkate alınmalıdır. Bu çalışmada amacımız; Balb/c türü dişi farelerde kafes popülasyonu yoğunluğunun östrus siklusu ve vajinal sürüntü sitolojisi üzerine etkilerini incelemektir. Hayvanlar bir kafeste iki (Set I, n=40) ya da sekiz fare (Set II, n=40) olacak şekilde kafeslenerek 2 deney seti oluşturuldu. Her bir dişi farenin östrus durumu, 15 gün boyunca her sabah standart vajinal sürüntü metoduyla tespit edildi. İki dişi/kafes grubunda ortalama 4-5 günlük düzenli sikluslar gözlenirken, 8 dişi/kafes grubunda uzayan sikluslar tespit edildi. Set I grubunda her faza ait vajinal sürüntü histolojisi normal hücre popülasyonunu sergilemekte idi. Kalabalık kafes popülasyonunda ise nötrofillerden ve mukustan zengin sürüntüler elde edildi. Deney süresince Set II grubunda östrus ve proöstrus faz frekansları anlamlı oranda azalırken, diöstrus frekansı belirgin derecede artmıştı. Aynı şekilde diöstrus indeksi-nin de Set II grubunda Set I grubundan anlamlı oranda yüksek olduğu gözlendi. Sonuç olarak, elde ettiğimiz bulgular Balb/c türü dişi fareler kullanılırken, östrus senkronizasyonu için kafes yoğunluğunun 2 dişi/kafes şeklinde düzenlenmesi ile hem düzenli siklusların hem de normal vajinal sürüntü histolojisinin elde edilebileceğini göstermektedir.Öğe Cisplatin nefrotoksisitesinde E vitamininin koruyucu etkileri: ışık ve elektron mikroskopik çalışma(2007) Kanter, Mehmet; Tarladaşalışır, Yeter Topçu; Uygun, MüberraBu çalışmada, cisplatin nefrotoksisitesinde E vitamininin koruyucu etkisinin bulunup bulunmadığının morfolojik düzeyde incelenmesi amaçlandı. Gereç ve Yöntem: Çalışmada, 18 adet erkek Wistar albino sıçan 3 gruba ayrıldı. Kontrol grubu deneklerine üç ay boyunca her gün intramusküler (im) yolla serum fizyolojik verilirken, II. ve III. grup deneklerine ise üç ay boyunca, ayda bir kez 5 mg/kg cisplatin intravenöz (iv) yolla uygulandı. III. grup deneklere ayrıca, ilk cisplatin enjeksiyonunu takiben deney boyunca her gün 5 mg/kg E vitamini im yolla verildi. Çalışmanın sonunda, tüm deneklerin böbrek korteks nekropsi materyalleri alınarak ışık ve elektron mikroskopik düzeyde incelendi. Bulgular: Kontrol grubu deneklerde; böbrek dokusunun normal yapıda olduğu gözlendi. Cisplatin grubunda ise bir çok böbrek cisimciğinin normal görünüm ve büyüklüğünü kaybetmiş olduğu, bazılarının ortadan kaybolduğu ve bu alanlara lenfositlerin infiltre olduğu görüldü. Ayrıca glomerüler yapının ise kısmen veya tamamen ortadan kalktığı dikkati çekti. Özellikle, kor-tikomedüller bölgede olmak üzere, tübüler genişlemelere ve tübüler hasarlara rastlandı. Proksimal tübül dejenerasyonları; bazal membran kalınlaşması, prizmatik hücre fırçamsı kenarlarında bozulma, vakuolizasyon, mitokondride şişme şeklinde olup, intersitisyel bağ dokuda da bir çok infiltrasyon sahası gözlendi. E vitamini ile tedavi edilen grupta morfolojik hasarların ve morfometrik değişikliklerin azaldığı buna karşın infiltrasyon odaklarının azalmakla birlikte yer yer varlığını sürdürdüğü dikkati çekti.Sonuç: Bu çalışmada, sıçanlarda cisplatin enjeksiyonları ile oluşturulan nefrotoksisitenin önlenmesinde, uzun süreli E vitamini tedavisinin morfolojik açıdan etkili olduğunu gördük. Bu bilgiler ışığında cisplatin kemoterapisi esnasında tedaviye E vitamini eklenmesinin klinik açıdan fayda sağlayacağı kanısındayız.Öğe Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats(Elsevier Gmbh, 2007) Altan, Mehmet Fatih; Kanter, Mehmet; Donmez, Senayi; Kartal, Murat Emre; Buyukbas, SadikExtracts of the seeds of Nigella sativa (NS), an annual herbaceous plant of the Ranunculaceae family, have been used for many years for therapeutic purposes, including their potential anti-diabetic properties. The aim of the present study was to test the hypothesis that combined treatment with NS and human parathyroid hormone (hPTH) is more effective than treatment with NS or hPTH atone in improving bone mass, connectivity, biomechanical behaviour and strength in insulin-dependent diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) at a single dose of 50mg/kg. The diabetic rats received NS (2 ml/kg/day, i.p.), hPTH (6 mu g/kg/clay, i.p.) or NS and hPTH combined for 4 weeks, starting 8 weeks after STZ injection. The beta-cells of the pancreatic islets of Langerhans were examined by immunohistochemical methods. In addition, bone sections of femora were processed for histomorphometry and biomechanical. analysis. In diabetic rats, the beta-cells were essentially negative for insltin-immunoreactivity. NS treatment (alone or in combination with hPTH) significantly increased the area of insulin immunoreactive beta-cells in diabetic rats; however, hPTH treatment atone only led to a slightly increase in the insulin-immunoreactivity. These results suggest that NS might be used in a similar manner to insulin as a safe and effective therapy for diabetes and might be useful in the treatment of diabetic osteopenia. (c) 2007 Elsevier GmbH. All rights reserved.Öğe Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats(Wiley-Blackwell, 2013) Kanter, Mehmet; Aktas, Cevat; Erboga, MustafaScope: The present study was designed to examine the protective and antioxidative effects of curcumin (Cur) on streptozotocin (STZ) induced testicular damage, apoptotic germ cell death, and oxidative stress. Methods and resultsDiabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg). The rats in the Cur-treated group were given Cur (100 mg/kg) once a day intragastrik for 8 weeks starting 3 days prior to STZ injection. Cur treatment significantly decreased the elevated tissue malondialdehyde levels and increased the reduced superoxide dismutase, and glutathione peroxidase enzyme activities in testis tissues samples. The Cur-treated rats in the diabetic group showed an improved histological appearance and serum testosterone levels. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling and there was a rise in the expression of proliferating cell nuclear antigen in testis tissues of Cur-treated rats in the diabetic group. ConclusionThese results demonstrate that Cur attenuated testicular damage in diabetic rats by decreasing oxidative stress.Öğe Depression of glucose levels and partial restoration of pancreatic ?-cell damage by melatonin in streptozotocin-induced diabetic rats(Springer Heidelberg, 2006) Kanter, Mehmet; Uysal, Hamdi; Karaca, Turan; Sagmanligil, Hulya OzdemirDiabetes mellitus is a common but serious metabolic disorder associated with many functional and structural complications. Glucose metabolism is disturbed due to an absolute or relative insulin deficiency. The experiment was carried out to determine the effect of melatonin on blood glucose and insulin concentrations, and histopathology of pancreatic beta-cells in streptozotocin (STZ)-induced diabetic rats. The rats were randomly allocated into one of the four experimental groups: group A (control), group B (diabetic untreated), group C (diabetic treated with melatonin for 6 weeks) and group D (diabetic treated with melatonin for 8 weeks); each group contained ten animals. Diabetes was induced in B, C and D groups by a single intraperitoneal (i.p.) injection of STZ (50 mg/kg, freshly dissolved in 5 mmol/l citrate buffer, pH 4.5). The rats in melatonin-treated groups were subjected to the daily i.p injection of 10 mg kg(-1) of melatonin for 6 or 8 weeks starting the day after STZ injection. Control and diabetic untreated rats were injected with the same volume of isotonic NaCl as the melatonin treated groups. Almost all insulin-positive beta-cells were degranulated, degenerated or necrotic in the STZ-treated rats leading to decrease in insulin secretion and an increase in blood glucose concentration. Melatonin treatment caused a sharp decrease in the elevated serum glucose, a slight increase in the lowered serum insulin concentrations and small partial regeneration/proliferation of beta-cells of islets. It is concluded that the hypoglycemic action of melatonin could be partly due to small amelioration in the beta-cells of pancreatic islets causing a slight increase in insulin secretion, it is mostly due to the extrapancreatic actions of the melatonin.Öğe EBSELEN PROTECTS AGAINST OXIDATIVE AND MORPHOLOGICAL EFFECTS OF HIGH CONCENTRATION CHRONIC TOLUENE EXPOSURE ON RAT SCIATIC NERVES(Modestum Ltd, 2006) Coskun, Omer; Yuncu, Mehmet; Kanter, Mehmet; Buyukbas, SadikAim: Neuroprotective effects of ebselen against free radical damage have been studied extensively. Toluene generates reactive oxygen species (ROS) and the toxic effects relating to these reactants. The aim of this study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Methods: We also examined the protective effects of ebselen against toluen exposure. Male Wistar albino rats (150-250 g) were divided in three experimental groups: the control, toluene and toluene+ ebselen treated group (n= 10 for each). Toluene treatment was performed by inhalation of 3000 ppm toluene, in a 8 hr/day and 6 day/week order for 16 weeks. Control group received 1ml serum physiologic and ebselen was given i. p. (10 mg/kg) to toluene+ ebselen treated group just after toluene exposure per day. Blood and tissue samples were obtained for biochemical and histopathological investigation. The blood and sciatic nerves were assayed for toluene by gas chromatography. Results: Toluene significantly increased blood and tissue malondialdehyde (MDA), and decreased tissue superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), but not tissue catalase (CAT) levels when compared with controls. Ebselen administration prevented to increase of blood and tissue MDA and decrease to SOD and GSH-Px levels induced by toluene inhalation. Electron micrographs of sciatic nerve in the toluene group shows myelin destructions with onion-bulb and bubble form protrusion on the myelin sheath and axolemma border of myelinated axons. Furthermore, ebselen salvaged the nerve fibers from toluene exposure. Conclusion: Ebselen treatment may provide neuroprotection against toluene neurotoxicity by directly scavenging ROS and by indirectly augmenting their antioxidant capacity.Öğe Effect of black cumin (Nigella sativa) on heart rate, some hematological values, and pancreatic ?-cell damage in cadmium-treated rats(Humana Press Inc, 2006) Demir, Halit; Kanter, Mehmet; Coskun, Omer; Uz, Yesim Hulya; Koc, Ahmet; Yildiz, AbdulmelikThis study was designed to investigate the effect of Nigella saliva (NS) on the heart rate, some hematological values, and pancreatic beta-cell damage in cadmium (Cd)-treated rats. The rats were randomly grouped into one of three experimental groups: Control, Cd treated, and Cd + NS treated. Each group contained 10 animals. The Cd-treated and Cd + NS-treated groups were injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl in the amount of 2 mL/kg for 30 d, resulting in a dosage of 0.49 mg Cd/kg/d. The control group was injected with only isotonic NaCl (2 mL/kg/d) throughout the experiment (for 30 d). Three days prior to administration of CdCl2, the Cd + NS-treated group received the daily intraperitoneal (ip) injection of 2 mL/kg NS until the end of the study; animals in all three groups were fasted for 12 h and blood samples were taken for the determination of the glucose and insulin levels, red blood cell (RBC) and white blood cell (WBC) counts, packet cell volume (PCV), and hemoglobin (Hb) concentration. The heart rates of rats were also measured by a direct writing electrocardiograph before the blood withdrawals. It was found that NS treatment increased the lowered insulin levels, RBC and WBC counts, PCV, and neutrophil percentage in Cd-treated rats. However, the WBC count of Cd-treated rats with NS treatment was still lower than those of control values. NS treatment also decreased the elevated heart rate and glucose concentration of Cd-treated rats. Pancreatic tissues were also harvested from the sacrificed animals for morphological and immunohistochemical examinations. Cd exposure alone caused a degeneration, necrosis, and weak degranulation in the beta-cells of the pancreatic islets. In Cd + NS-treated rats, increased staining of insulin and preservation of islet cells were apparent. It is concluded that NS treatment might decrease the Cd-treated disturbances on heart rate, some hematological values, and pancreatic beta-cell.Öğe Effect of curcumin on ipsilateral and contralateral testes after unilateral testicular torsion in a rat model(Karger, 2008) Basaran, Umit Nusret; Dokmeci, Dikmen; Yalcin, Omer; Inan, Mustafa; Kanter, Mehmet; Aydogdu, Nurettin; Turan, NesrinObjective: The aim of the study was to determine the protective effect of curcumin on testicular ischemia- reperfusion ( I/ R) injury. Materials and Methods: 32 male rats were divided into four groups ( n = 8): group 1: control; group 2: ischemia; group 3: I/ R, and group 4: I/ R+CUR. Curcumin ( 150 mg/ kg, p. o.) was administered before 30 min of reperfusion in group 4. Malondialdehyde ( MDA) levels, Johnsen's testicular biopsy scores, and mean seminiferous tubule diameter measurements were evaluated in testes. In addition, endothelial nitric oxide synthase ( eNOS) and inducible nitric oxide synthase ( iNOS) expressions were evaluated immunohistochemically. Results: MDA levels in control groups were significantly lower than other groups in ipsilateral and contralateral testes. Johnsen's scores in the control group were significantly higher than in other groups. MDA levels and Johnsen's scores in the I/ R+ CUR group were similar to the ischemia and I/ R groups in ipsilateral and contralateral testes. The immunoreactivity of iNOS and eNOS were increased in I/ R ipsilateral testicular groups. After I/ R, iNOS and eNOS expression increased slightly in contralateral groups. Additionally, the curcumin treatment decreased iNOS and eNOS immunoreactivity in ipsilateral and contralateral testes. Conclusion: The results suggest that curcumin did not protect the unilateral nor contralateral testes. This observation may depend on inhibition of iNOS and eNOS due to inhibition of the antioxidant, anti- inflammatory effects of nitric oxide. Copyright (C) 2008 S. Karger AG, Basel.Öğe Effect of formaldehyde inhalation on rat livers: A light and electron microscopic study(Sage Publications Inc, 2010) Cikmaz, Selman; Kutoglu, Tunc; Kanter, Mehmet; Mesut, RecepIt is well known that formaldehyde (FA) is cytotoxic and potentially carcinogenic. Although the individual effects of this reactant on cells has been investigated, the cytotoxicity exerted by the coexistence of FA is poorly understood. The aim of this study was to investigate the effects of FA on the liver in rats, by light and electron microscopic level. We used 18 Wistar albino rats divided into three groups, exposed to 0 (control), 19.7 ppm FA gas for a total of 4 weeks, 8 h/day, 5 days a week (subacute) and 20.3 parts per million (ppm) FA gas for a total of 13 weeks, 8 h/day, 5 days a week (subchronic). After the completion of the exposure period, they were sacrificed by decapitation and their liver tissue samples were taken in order to be processed for light and electron microscopic studies. Light microscopic evaluation of liver tissue samples of FA-exposed rats revealed enlarged sinusoids filled with blood and mononuclear cell infiltration in the portal areas and around the central veins. In addition, some of the hepatocytes showed loss of cytoplasm, and some had a hyperchromatic nucleus. The cells of FA-exposed livers, on the other hand, showed an electron-lucent ground-cytoplasm and a hypertrophy of the smooth-surfaced endoplasmic reticulum. In conclusion, we observed that exposure FA caused diverse histopathological changes indicating the destruction in the liver tissue and this destruction has direct relationship with the length of the exposure period.Öğe Effect of Ginkgo biloba extract (EGb 761) on the healing of left colonic anastomoses in rat(Wiley, 2007) Kisli, Erol; Ozdemir, Huelya; Kosem, Mustafa; Surer, Hatice; Ciftci, Ali; Kanter, MehmetBackground The aim of this study was to assess the effect of Ginkgo biloba extract (EGb 761) on healing of experimental colonic anastomoses in a rat model. Methods Rats were divided into four groups: postoperative day (POD) 3 untreated control group, POD 3 EGb 761 group, POD 7 untreated control group, and POD 7 EGb 761 group. In the oral EGb 761 groups, the agent was given at 9.6 mg daily per orogastric route using a 4-F fine feeding catheter. We measured bursting pressures and hydroxyproline content and histologically examined the resected anastomoses on POD 3 and POD 7. Results The bursting pressures increased more in the EGb 761 group than in the untreated control group on POD 3, but this difference was not statistically significant. Hydroxyproline content was higher in the EGb 761 group than in the untreated control group on POD 3, and this difference was statistically significant. Anastomosis bursting pressure values and hydroxyproline contents were significantly higher in the EGb 761 group than in the untreated control group on POD 7. Histological examination showed greater fibroblastic activity in the EGb 761 group than in the untreated control group on POD 3. There was no significant difference in anastomotic polimorphonuclear leukocyte, mononuclear cells and blood vessel neodevelopment between the POD 3 groups, but there was significant difference in fibroblastic activity and blood vessel neodevelopment between the POD 7 groups. Conclusions These results showed us that EGb 761 administration resulted in enhanced stability of colonic anastomoses during the first postoperative week.Öğe The effect of hyperbaric oxygen treatment on aspiration pneumonia(Springer, 2011) Sahin, Sevtap Hekimoglu; Kanter, Mehmet; Ayvaz, Suleyman; Colak, Alkin; Aksu, Burhan; Guzel, Ahmet; Basaran, Umit NusretWe have studied whether hyperbaric oxygen (HBO) prevents different pulmonary aspiration materials-induced lung injury in rats. The experiments were designed in 60 Sprague-Dawley rats, ranging in weight from 250 to 300 g, randomly allotted into one of six groups (n = 10): saline control, Biosorb Energy Plus (BIO), hydrochloric acid (HCl), saline + HBO treated, BIO + HBO treated, and HCl + HBO treated. Saline, BIO, HCl were injected into the lungs in a volume of 2 ml/kg. A total of seven HBO sessions were performed at 2,4 atm 100% oxygen for 90 min at 6-h intervals. Seven days later, rats were sacrificed, and both lungs in all groups were examined biochemically and histopathologically. Our findings show that HBO inhibits the inflammatory response reducing significantly (P < 0.05) peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Pulmonar aspiration significantly increased the tissue HP content, malondialdehyde (MDA) levels and decreased (P < 0.05) the antioxidant enzyme (SOD, GSH-Px) activities. HBO treatment significantly (P < 0.05) decreased the elevated tissue HP content, and MDA levels and prevented inhibition of SOD, and GSH-Px (P < 0.05) enzymes in the tissues. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase, TUNEL and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with HBO therapy. It was concluded that HBO treatment might be beneficial in lung injury, therefore, shows potential for clinical use.Öğe The effect of magnetic field therapy and electric stimulation on experimental burn healing(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2019) Keskin, Yasar; Tastekin, Nurettin; Kanter, Mehmet; Top, Husamettin; Ozdemir, Ferda; Erboga, Mustafa; Taspinar, OzgurObjectives: : In this study, we aimed to compare morphological and histological differences between magnetic field and electric stimulation therapies in an experimental burn injury model in rats. Materials and methods: Between February 2011 and July 2011, a total of 21 Sprague-Dawley female rats were used in this study. Second-degree burns were induced on the back areas of the rats. All rats were equally divided into three groups including seven in each: the first burn group was treated with antibacterial pomade (Group 1, control group); the second group was treated with both antibacterial pomade and pulsed electromagnetic field therapy (Group 2); and the third group was treated with antibacterial pomade and electric stimulation for 14 days (Group 3). Results: Earlier re-epithelialization, wound area contraction, reduction of edema, and hyperaemia were observed on gross examination in the pulsed electromagnetic fields and electric stimulation therapy groups compared to the control group. Neovascularization, collagen density, granulation tissue formation, cell proliferation, and inflammatory cell response of the pulsed electromagnetic fields and electric stimulation group increased, compared to the control group, in the histopathological evaluation (p<0.05). Conclusion: Our study results showed the positive healing effects of electric stimulation and pulsed electromagnetic fields on burn injury. Pulsed electromagnetic fields therapy produced more positive signs of healing than the electric stimulation group.Öğe The effect of methylene blue treatment on aspiration pneumonia(Academic Press Inc Elsevier Science, 2015) Kanter, Mehmet; Sahin, Sevtap Hekimoglu; Basaran, Umit Nusret; Ayvaz, Suleyman; Aksu, Burhan; Erboga, Mustafa; Colak, AlkinBackground: The study aimed to examine whether methylene blue (MB) prevents different pulmonary aspiration materials-induced lung injury in rats. Methods: The experiments were designed in 60 Sprague-Dawley rats, ranging in weight from 250-300 g, randomly allotted into one of six groups (n = 10): saline control, Biosorb Energy Plus (BIO), hydrochloric acid (HCl), saline + MB treated, BIO + MB treated, and HCl + MB treated. Saline, BIO, and HCl were injected into the lungs in a volume of 2 mL/kg. After surgical procedure, MB was administered intraperitoneally for 7 days at a daily dose of 2 mg/kg per day. Seven days later, rats were killed, and both lungs in all groups were examined biochemically and histopathologically. Results: Our findings show that MB inhibits the inflammatory response reducing significantly (P < 0.05) peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Pulmonary aspiration significantly increased the tissue hydroxyproline content, malondialdehyde levels, and decreased (P < 0.05) the antioxidant enzyme (superoxide dismutase and glutathione peroxidase) activities. MB treatment significantly (P < 0.05) decreased the elevated tissue hydroxyproline content and malondialdehyde levels and prevented the inhibition of superoxide dismutase and glutathione peroxidase (P < 0.05) enzymes in the tissues. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase (iNOS), terminal deoxynucleotidyl transferase dUTP nick end labeling, and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with MB therapy. Conclusions: MB treatment might be beneficial in lung injury and therefore shows potential for clinical use. (C) 2015 Elsevier Inc. All rights reserved.
