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Öğe The comparison of electron microscopy and scintigraphy in determining the protective effect of dimethylsulphoxide (DMSO) on ischemia/reperfusion injury through pringle maneuver(H G E Update Medical Publishing S A, 2001) Hatipoglu, AR; Temiz, E; Yüksel, M; Hoscoskun, Z; Coskun, I; Hüseyinova, GBackground/Aims: We investigated the role of the electron microscopy and hepatobiliary scintigraphy in determining the effect of DMSO (dimethysulphoxide) and ischemia/reperfusion injury in the liver after the Pringle maneuver. Methodology: Twenty-four rabbits were divided into the following groups; A: Control group, B: Pringle, C: 10mg/kg DMSO, D: 1g/kg DMSO + Pringle. Group A was considered as a control group and only laparotomy was applied. Group B was exposed to Pringle maneuver only. Group C:was given 10mg/kg of DMSO via the vena cava inferior. Group D was given 1g/kg of DMSO- A clamp was fastened for the groups of B, C and D in the 30th minute of the Pringle maneuver and a biopsy was applied five minutes later. Fifteen minutes later a dynamic hepatobiliary scintigraphy was applied. From dynamic images, liver peak time and activity half time of the liver were obtained. Results: It was found that liver peak time and liver activity half time values of the group B, C and D were significantly longer than group A. Liver peak time and liver activity half time values of group B was not different from group C. However, some values of group D were found to be significantly shorter than groups B and C. In the electron microscopy examination, only in group B were some specific degenerative changes observed in the sinusoids. We observed less irreversible changes in group C than in group B. Oh the other hand, the least irreversible changes were in group D. Conclusions: As a conclusion, while electron microscopy is regarded as the gold standard, hepatobiliary scintigraphy may be thought of as an easily applicable: method in determining the ischemic reperfusion injury in the clinical comparison of the protective agents.Öğe The effect of nadroparine on coagulation mechanisms(Lippincott Williams & Wilkins, 2003) Sunar, H; Hüseyinova, G; Canbaz, S; Halici, Ü; Duran, ELow molecular weight heparins are widely used in the prophylaxis and treatment of thrombotic disorders. The effect of low molecular weight heparins on coagulation was examined ultrastructurally in an animal model. A test and a control group was formed, each consisting of five rabbits. Nadroparine (225 Institute of Chaoy Unit/kg twice daily) was applied to the test group for 10 days. The control group received 1 ml saline solution subcutaneously. Blood and vascular tissue samples collected at the end of the 10th day were evaluated under a JEM 100 B electron microscope. Platelet degranulation and agglutination was observed in the control group. Fibrin materials were detected in the cytoplasms and surroundings of degranulated platelets. Erythrocyte accumulation was remarkable on the vascular endothelium with intact coagulation periods. In the test group, outer membranes of platelets, hyalomere, and granular structures in the granulomeres were detected to be nearly intact. There were rare erythrocytes in the large vascular lumens. The aggregation phase had occurred but no agglutination was detected. Nadroparine seems to preserve consistency of lipoprotein membranes of platelets and granular structures containing enzymes, which contribute to the coagulation mechanisms. (C) 2003 Lippincott Williams Wilkins.Öğe Evaluation of liver parenchimal damage after pringle manoeuvre and protective effect of DMSO using hepatobiliary scintigraphy(Springer Verlag, 1999) Yüksel, M; Hatipoglu, A; Temiz, E; Salihoglu, YS; Hüseyinova, G; Berkarda, S[Abstract Not Available]Öğe The role of hepatobiliary scintigraphy in the evaluation of the protective effects of dimethylsulphoxide in ischaemic/reperfusion injury of liver(Lippincott Williams & Wilkins, 2000) Yüksel, M; Hatipoglu, A; Temiz, E; Salihoglu, YS; Hüseyinova, G; Berkarda, SLiver ischaemia may lead to parenchymal damage depending on the duration of the ischaemia. Dimethylsulphoxide (DMSO), a well-known radical oxygen scavenger, is a protective agent against ischaemia/reperfusion injury. In this study we aimed to investigate the role of hepatobiliary scintigraphy (HBSc) in detecting the protective effect of DMSO. Eighteen rabbits, in three groups of six, were injected with 37 MBq technetium-99m-mebrofenin via the ear veins. Dynamic scintigrams were taken for 60 min (1 frame/min). In group A, HBSc was performed without any surgery. In groups B and C the Pringle manoeuvre (PM) was applied for 30 min, and tissue specimens for electron microscopy were taken from the liver parenchyma 5 min after the end of the PM. In addition, in group C 1 g/kg DMSO was injected into each rabbit 5 min before application of the PM. HBSc was then performed in groups 5 and C. From the dynamic images time-activity curves (TACs) were obtained for each group, and the time of peak uptake (TPU) and time for half of the activity to clear from the liver (T 1/2) were calculated. The TPU and T 1/2 of group B were significantly longer than those of groups A and C (P <0.0005 and P < 0.005 for TPU, and P<0.0005 and P<0.02 for T 1/2 respectively). The TPU and T 1/2 of group C were significantly longer than those of group A (P <0.005 and P<0.02, respectively). While the electron microscopic images in group C showed reversible changes, those in group B showed both irreversible and reversible changes. The electron microscopic findings of groups B and C confirmed the scintigraphic findings. In conclusion, HBSc might be used as a practical quantitative method for detecting the protective effects of DMSO. However, its clinical value should be evaluated by further studies with human subjects. ((C) 2000 Lippincott Williams & Wilkins).
