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    Determination of plasma microRNA for early detection of gastric cancer
    (Springer, 2013) Gorur, Aysegul; Fidanci, Senay Balci; Unal, Nil Dogruer; Ayaz, Lokman; Akbayir, Serin; Yaroglu, Hatice Yildirim; Dirlik, Musa
    Gastric cancer is the fourth most prevalent malignancy worldwide and remains the second most common cause of cancer-related death globally. Understanding the molecular structure of gastric carcinogenesis might identify new diagnostic and therapeutic strategies for this disease. Thus, early detection of gastric cancer is a key measure to reduce the mortality and improve the prognosis of gastric cancer. There have recently been several reports that microRNAs (miRNAs) circulate in highly stable, cell-free forms in blood. Because serum and plasma miRNAs are relatively easy to access, circulating miRNAs also have great potential to serve as non-invasive biomarkers. Although a number of miRNAs associated with gastric cancer have been identified, the underlying mechanism of these miRNAs in tumorigenesis and tumor progression remains to be investigated. The purpose of this study is to identify the potential of serum miRNAs as biomarkers for early detection of gastric cancer patients. RNA was isolated using the High Pure miRNA Isolation Kit (Roche) following the manufacturer's protocol. cDNA and preamplification protocols were obtained from the isolated plasma miRNAs. The BioMark (TM) 96.96 Dynamic Array (Fluidigm Corporation) for real-time qPCR was used to simultaneously quantite the expression of 740 miRNAs. All statistical analyses were performed using the Biogazelle's qbase PLUS 2.0 software. In this study, among 740 miRNAs that we analyzed only miR-195-5p was significantly (p < 0.05, fold changes = 13, 3) down-regulated in gastric cancer patients compared with control. We demonstrated that miR-195-5p is a novel tumor suppressor miRNA and may contribute to gastric carcinogenesis. The miRNA expression profile described in this study should contribute to future studies on the role of miRNAs in gastric cancer.
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    Differential expression of microRNAs in plasma of patients with laryngeal squamous cell carcinoma: potential early-detection markers for laryngeal squamous cell carcinoma
    (Springer, 2013) Ayaz, Lokman; Gorur, Aysegul; Yaroglu, Hatice Yildirim; Ozcan, Cengiz; Tamer, Lulufer
    Altered microRNA (miRNA) expression has been found in many cancers, including lung, breast, prostate, bladder and colorectal cancer. Many recent studies have demonstrated that aberrant plasma miRNAs were also found in various types of cancers. However, the alteration in plasma miRNA expressions in laryngeal squamous cell carcinoma (LSCC) remains unclear. The present study aimed to investigate the alterations in plasma miRNAs in LSCC. In the present study, the expression profiles of 738 miRNAs in plasma from 20 patients and 44 healthy subjects were evaluated using high-throughput real-time quantitative polymerase chain reaction. Our results demonstrated that expression levels of 17 miRNAs were significantly upregulated in patients with LSCCs when compared to control group (p < 0.05). Expression levels of nine miRNAs were found significantly downregulated in LSCC patients (p < 0.05). In addition, 17 miRNAs were expressed only in LSCC group, and five of these miRNAs (miR-331-3p, 603, 1303, 660-5p and 212-3p) are LSCC specific and never seen before in plasma of any human subject. In conclusion, our study suggests that detecting these LSCC-specific miRNAs in plasma might serve as novel noninvasive biomarkers for LSCC.
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    MicroRNAs EXPRESSION PROFILES IN NON-SMALL CELL LUNG CANCER
    (Parlar Scientific Publications (P S P), 2021) Yaroglu, Hatice Yildirim; Akbayir, Serin; Gorur, Aysegul; Balci, Senay; Unal, Nil; Ayaz, Lokman; Ayan, Erhan
    MicroRNAs (miRNAs) are a new endogenous small non-coding RNA family that arranges expression of a few genes related in normal development as well as human diseases such as cancer. miRNAs are developing as potential diagnostic and therapeutic markers with deregulated expression in various cancers including lung cancer. In this study, we aimed to find out miRNA expression profiles and to show the relationship between non-small cell lung cancer and microRNAs levels, if there is. In the present study, the expression profiles of 740 miRNA in plasma from 31 patients and 64 healthy subjects were evaluated using high-throughput real-time quantitative polymerase chain reaction. All statistical analyses were performed using the BiogazelIe's qbase PLUS 2.0 software. Our results showed that expression levels of 7 microRNAs (miR-20b-5p, -30c, -146a, -192-5p, -206, -484, -574-3p) were significantly upregulated in patients when compared to control group (p<0.05). Expression levels of five miRNAs (miR-24-3p, -30a-5p, -106b-5p, -223-3p, -331-5p) were found significantly downregulated in lung cancer patients (p<0.05). Conclusion: In conclusion, our result showed that up -regulated and down-regulated miRNAs may be important role of early detection in non-small cell lung cancer.